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"@id": "https://doi.org/10.6084/m9.figshare.16918554.v1",
"url": "https://tandf.figshare.com/articles/figure/_i_Astragalus_i_polysacharin_inhibits_hepatocellular_carcinoma-like_phenotypes_in_a_murine_HCC_model_through_repression_of_M2_polarization_of_tumour-associated_macrophages/16918554/1",
"additionalType": "Figure",
"name": "Astragalus polysacharin inhibits hepatocellular carcinoma-like phenotypes in a murine HCC model through repression of M2 polarization of tumour-associated macrophages",
"author": [
{
"name": "Chun Li",
"givenName": "Chun",
"familyName": "Li"
},
{
"name": "Xin-You Pan",
"givenName": "Xin-You",
"familyName": "Pan"
},
{
"name": "Mingyun Ma",
"givenName": "Mingyun",
"familyName": "Ma"
},
{
"name": "Jun Zhao",
"givenName": "Jun",
"familyName": "Zhao"
},
{
"name": "Fengda Zhao",
"givenName": "Fengda",
"familyName": "Zhao"
},
{
"name": "Ya-Ping Lv",
"givenName": "Ya-Ping",
"familyName": "Lv"
}
],
"description": "Astragalus polysaccharin (APS), an extract of Astragalus propinquus Schischk, exerts antitumor effects in hepatocellular carcinoma (HCC). This study investigated the mechanism of action of APS in HCC. Tumour-associated macrophages (TAMs) were treated with APS (0, 8, 16 mg/mL) for 24 h. APS (16 mg/mL)-treated TAMs were co-cultured with MHCC97H/Huh7 cells for 24 h. Finally, BALB/c nude mice were divided into PBS, APS (50 mg/kg), APS (100 mg/kg), APS (200 mg/kg) groups: mice were inoculated with Huh7 cells to construct tumour xenograft model, followed by administration of APS (50, 100, 200 mg/kg) or PBS daily for 30 days. Cell proliferation, migration, invasion, tumour growth, macrophage markers and proportions were measured. APS 16 mg/mL treatment enhanced the expression of M1 macrophage markers (iNOS, IL-1β and TNF-α) and M1 macrophage proportions, while reducing the expression of M2 macrophage markers (IL-10, Arg-1) and M2 macrophage proportions in TAMs. Moreover, the APS-mediated M1 phenotype of TAMs significantly repressed cell proliferation, migration and invasion of MHCC97H and Huh7 cells. Moreover, APS (50, 100, 200 mg/kg) enhanced M1 macrophage proportions and reduced M2 macrophage proportions in the tumour tissues, and thus inhibited tumour growth of HCC. APS inhibits HCC-like phenotypes in a murine HCC model through repression of M2 polarization of TAMs. This work provides a novel theoretical basis for the application of APS in the clinical treatment of HCC.",
"license": "https://creativecommons.org/licenses/by/4.0/legalcode",
"keywords": "Biochemistry, Microbiology, FOS: Biological sciences, Chemical Sciences not elsewhere classified, Biological Sciences not elsewhere classified, Cancer, Plant Biology",
"contentSize": "326598 Bytes",
"dateCreated": "2021-11-02",
"datePublished": "2021",
"dateModified": "2024-03-21",
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"@id": "https://doi.org/10.6084/m9.figshare.16918554",
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"@id": "https://doi.org/10.1080/13880209.2021.1991384",
"@type": "ScholarlyArticle"
}
},
"schemaVersion": "http://datacite.org/schema/kernel-4",
"publisher": {
"@type": "Organization",
"name": "Taylor & Francis"
},
"provider": {
"@type": "Organization",
"name": "datacite"
}
}