{
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"@id": "https://doi.org/10.17044/scilifelab.14730921",
"url": "https://scilifelab.figshare.com/articles/figure/Simultaneous_visualization_of_DNA_loci_in_single_cells_by_combinatorial_multi-color_iFISH_Dataset_8_iFISH_chr10_spotting_Replicate_1_/14730921",
"additionalType": "Figure",
"name": "Simultaneous visualization of DNA loci in single cells by combinatorial multi-color iFISH (Dataset 8: iFISH chr10 spotting Replicate 1)",
"author": [
{
"name": "Ana Faustino Mota",
"givenName": "Ana",
"familyName": "Faustino Mota"
},
{
"name": "Maud Schweitzer",
"givenName": "Maud",
"familyName": "Schweitzer"
},
{
"name": "Magda Bienko",
"givenName": "Magda",
"familyName": "Bienko"
}
],
"description": "We designed 13 probes targeting 13 consecutive loci on chromosome 10 using iFISH4U. The 23 loci are spaced with 10 Mb. There are 2 probes with AF488 dye, 2 probes with AT542, 3 probes with AF594, 2 probes with AT647N, 2 probes with AF700 and 2 probes with AF790. Replicate 1
Experiment Imaging Method: epifluorescence microscopy
Protocol Name: iFISH protocol
Each dataset consists of multiple fields of view (FOVs) that were acquired with seven different dyes (AF488 labeled as ‘a488’; AT542 as ‘tmr’; AF594 as ‘a594’; AT647N as ‘Cy5’, AF700 as ‘a700’, AF790 as ‘ir800’; and Hoechst 33342 as ‘dapi’) and all the FOVs are provided as separate .tiff files. Each FOV comprises 81–95 focal planes spaced 0.2 μm apart and has a size of 1024x1024 pixels.
",
"license": "https://creativecommons.org/licenses/by/4.0/legalcode",
"keywords": "60407 Genome Structure and Regulation, FOS: Biological sciences",
"contentSize": "22968136583 Bytes",
"dateCreated": "2021-10-25",
"datePublished": "2021",
"dateModified": "2021-10-25",
"schemaVersion": "http://datacite.org/schema/kernel-4",
"publisher": {
"@type": "Organization",
"name": "SciLifeLab"
},
"provider": {
"@type": "Organization",
"name": "datacite"
}
}