{"data":{"id":"10.6084/m9.figshare.c.4283600.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.4283600.v1","prefix":"10.6084","suffix":"m9.figshare.c.4283600.v1","identifiers":[{"identifier":"https://doi.org/10.6084/m9.figshare.c.4283600.v1","identifierType":"DOI"}],"alternateIdentifiers":[{"alternateIdentifierType":"DOI","alternateIdentifier":"https://doi.org/10.6084/m9.figshare.c.4283600.v1"}],"creators":[{"name":"Canales, Arturo","nameType":"Personal","givenName":"Arturo","familyName":"Canales","affiliation":[],"nameIdentifiers":[]},{"name":"Zhuo, Yue","nameType":"Personal","givenName":"Yue","familyName":"Zhuo","affiliation":[],"nameIdentifiers":[]},{"name":"Amitrano, Andrea","nameType":"Personal","givenName":"Andrea","familyName":"Amitrano","affiliation":[],"nameIdentifiers":[]},{"name":"Minsoo Kim","affiliation":[],"nameIdentifiers":[]},{"name":"Hernandez-Aranda, Raul","nameType":"Personal","givenName":"Raul","familyName":"Hernandez-Aranda","affiliation":[],"nameIdentifiers":[]},{"name":"P. Scott Carney","affiliation":[],"nameIdentifiers":[]},{"name":"Schnell, Martin","nameType":"Personal","givenName":"Martin","familyName":"Schnell","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Accessible quantitative phase imaging in\nconfocal microscopy with sinusoidal-phase\nsynthetic optical holography"}],"publisher":"Figshare","container":{},"publicationYear":2018,"subjects":[{"subject":"90605 Photodetectors, Optical Sensors and Solar Cells","subjectScheme":"FOR"},{"subject":"FOS: Electrical engineering, electronic engineering, information engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"111302 Optical Technology","subjectScheme":"FOR"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2018-11-30","dateType":"Created"},{"date":"2018-11-30","dateType":"Updated"},{"date":"2018","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1364/ao.58.000a55","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.4283600","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC BY 4.0","rightsUri":"https://creativecommons.org/licenses/by/4.0"}],"descriptions":[{"description":"We present a technically simple implementation of quantitative phase imaging in\nconfocal microscopy based on synthetic optical holography with sinusoidal-phase reference\nwaves. Using a Mirau interference objective and low-amplitude vertical sample vibration with\na piezo-controlled stage, we record synthetic holograms on commercial confocal microscope\n(Nikon, model: A1R; Zeiss: model: LSM-880), from which quantitative phase images are\nreconstructed. We demonstrate our technique by stain-free imaging of cervical (HeLa), ovarian\n(ES-2) cancer cells and stem cell (mHAT9a) samples. Our technique has the potential to extend\nfluorescence imaging applications in confocal microscopy by providing label-free cell finding,\nmonitoring cell morphology as well as non-perturbing long-time observation of live cells based\non quantitative phase contrast.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/collections/Accessible_quantitative_phase_imaging_in_confocal_microscopy_with_sinusoidal-phase_synthetic_optical_holography/4283600/1","contentUrl":null,"metadataVersion":1,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2018-11-30T15:28:34.000Z","registered":"2018-11-30T15:28:35.000Z","published":"2018","updated":"2020-07-14T16:58:52.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figsha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