{"data":{"id":"10.6084/m9.figshare.c.4162766","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.4162766","prefix":"10.6084","suffix":"m9.figshare.c.4162766","identifiers":[{"identifier":"https://doi.org/10.6084/m9.figshare.c.4162766","identifierType":"DOI"}],"alternateIdentifiers":[{"alternateIdentifierType":"DOI","alternateIdentifier":"https://doi.org/10.6084/m9.figshare.c.4162766"}],"creators":[{"name":"Lanubile, Alessandra","nameType":"Personal","givenName":"Alessandra","familyName":"Lanubile","affiliation":[],"nameIdentifiers":[]},{"name":"Stagnati, Lorenzo","nameType":"Personal","givenName":"Lorenzo","familyName":"Stagnati","affiliation":[],"nameIdentifiers":[]},{"name":"Samayoa, Luis F.","nameType":"Personal","givenName":"Luis F.","familyName":"Samayoa","affiliation":[],"nameIdentifiers":[]},{"name":"Bragalanti, Mario","nameType":"Personal","givenName":"Mario","familyName":"Bragalanti","affiliation":[],"nameIdentifiers":[]},{"name":"Giorni, Paola","nameType":"Personal","givenName":"Paola","familyName":"Giorni","affiliation":[],"nameIdentifiers":[]},{"name":"Busconi, Matteo","nameType":"Personal","givenName":"Matteo","familyName":"Busconi","affiliation":[],"nameIdentifiers":[]},{"name":"Holland, James B.","nameType":"Personal","givenName":"James B.","familyName":"Holland","affiliation":[],"nameIdentifiers":[]},{"name":"Marocco, Adriano","nameType":"Personal","givenName":"Adriano","familyName":"Marocco","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Supplemental files from the study: A genome wide association study reveals markers and genes associated with resistance to Fusarium verticillioides infection of seedlings in a maize diversity panel"}],"publisher":"Figshare","container":{},"publicationYear":2018,"subjects":[{"subject":"70305 Crop and Pasture Improvement (Selection and Breeding)","subjectScheme":"FOR"},{"subject":"FOS: Agriculture, forestry and fisheries","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"70308 Crop and Pasture Protection (Pests, Diseases and Weeds)","subjectScheme":"FOR"}],"contributors":[],"dates":[{"date":"2018-07-12","dateType":"Created"},{"date":"2018-12-20","dateType":"Updated"},{"date":"2018","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC BY 4.0","rightsUri":"https://creativecommons.org/licenses/by/4.0"}],"descriptions":[{"description":"\u003cem\u003eFusarium\nverticillioides\u003c/em\u003e infects maize, causing ear rot, yield loss and\ncontamination by fumonisin mycotoxins. The fungus can be transmitted via kernels\nand cause systemic infection in maize. Maize resistance to the fungus may occur\nat different developmental stages, from seedling to maturity. Resistance during\nkernel germination is part of the plant-pathogen interaction and so far this\naspect has not been investigated. In the present study, a genome wide association\nstudy (GWAS) of resistance to \u003ci\u003eFusarium\u003c/i\u003e\nduring the seedling developmental stage was conducted in a maize diversity\npanel using 226,446 SNP markers. Seedling germination and disease phenotypes\nwere scored on artificially inoculated kernels using the rolled towel assay.\nGWAS identified 164 SNPs significantly associated with the traits examined.\nFour SNPs were associated with disease severity score after inoculation, 153\nwere associated with severity in asymptomatic kernels and 7 with the difference\nbetween the severity ratings in inoculated and non-inoculated seeds. A set of\ngenes containing or physically near the significant SNPs were identified as\ncandidates for \u003ci\u003eFusarium\u003c/i\u003e resistance at\nthe seedling stage. Functional analysis revealed that many of these genes are directly\ninvolved in plant defense against pathogens and stress responses, including transcription\nfactors, chitinase, cytochrome P450, and ubiquitination proteins. In addition,\n25 genes were found in high linkage disequilibrium with the associated SNPs identified\nby GWAS and four of them directly involved in disease resistance. These\nfindings contribute to understanding the complex system of maize-\u003ci\u003eF. verticillioides\u003c/i\u003e and may improve\ngenomic selection for \u003ci\u003eFusarium\u003c/i\u003e\nresistance at the seedling stage.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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