{"data":{"id":"10.6084/m9.figshare.c.3263785.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.3263785.v1","prefix":"10.6084","suffix":"m9.figshare.c.3263785.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Sakaki, Yohei","nameType":"Personal","givenName":"Yohei","familyName":"Sakaki","affiliation":[],"nameIdentifiers":[]},{"name":"Tashiro, Mitsuru","nameType":"Personal","givenName":"Mitsuru","familyName":"Tashiro","affiliation":[],"nameIdentifiers":[]},{"name":"Katou, Moe","nameType":"Personal","givenName":"Moe","familyName":"Katou","affiliation":[],"nameIdentifiers":[]},{"name":"Sakuma, Chiseko","nameType":"Personal","givenName":"Chiseko","familyName":"Sakuma","affiliation":[],"nameIdentifiers":[]},{"name":"Hirano, Takako","nameType":"Personal","givenName":"Takako","familyName":"Hirano","affiliation":[],"nameIdentifiers":[]},{"name":"Hakamata, Wataru","nameType":"Personal","givenName":"Wataru","familyName":"Hakamata","affiliation":[],"nameIdentifiers":[]},{"name":"Nishio, Toshiyuki","nameType":"Personal","givenName":"Toshiyuki","familyName":"Nishio","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Enzymatic synthesis of novel oligosaccharides from \u003ci\u003eN\u003c/i\u003e-acetylsucrosamine and melibiose using \u003ci\u003eAspergillus niger\u003c/i\u003e α-galactosidase, and properties of the products"}],"publisher":"Figshare","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Genetics"},{"subject":"Neuroscience"},{"subject":"Physiology"},{"subject":"Pharmacology"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"80699 Information Systems not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Computer and information sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Computer and information sciences","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2016-06-02","dateType":"Created"},{"date":"2016-06-02","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09168451.2016.1189316","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.3263785","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC-BY","rightsUri":"http://creativecommons.org/licenses/by/3.0/us"}],"descriptions":[{"description":"Two kinds of oligosaccharides, \u003ci\u003eN\u003c/i\u003e-acetylraffinosamine (RafNAc) and \u003ci\u003eN\u003c/i\u003e-acetylplanteosamine (PlaNAc), were synthesized from \u003ci\u003eN\u003c/i\u003e-acetylsucrosamine and melibiose using the transgalactosylation activity of \u003ci\u003eAspergillus niger\u003c/i\u003e α-galactosidase. RafNAc and PlaNAc are novel trisaccharides in which d-glucopyranose residues in raffinose (Raf) and planteose are replaced with \u003ci\u003eN\u003c/i\u003e-acetyl-d-glucosamine. These trisaccharides were more stable in acidic solution than Raf. RafNAc was hydrolyzed more rapidly than Raf by α-galactosidase of green coffee bean. In contrast, RafNAc was not hydrolyzed by \u003ci\u003eSaccharomyces cerevisiae\u003c/i\u003e invertase, although Raf was hydrolyzed well by this enzyme. These results indicate that the physicochemical properties and steric structure of RafNAc differ considerably from those of Raf. Two kinds of novel trisaccharides were synthesized from N-acetylsucrosamine and melibiose, using transgalactosylation action of \u003ci\u003eAspergillus niger\u003c/i\u003e α-galactosidase.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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