{"data":{"id":"10.6084/m9.figshare.c.2179191.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2179191.v1","prefix":"10.6084","suffix":"m9.figshare.c.2179191.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Utoomporn Surayot","affiliation":[],"nameIdentifiers":[]},{"name":"Lee, Ju Hun","nameType":"Personal","givenName":"Ju Hun","familyName":"Lee","affiliation":[],"nameIdentifiers":[]},{"name":"Chartchai Kanongnuch","affiliation":[],"nameIdentifiers":[]},{"name":"Yuwadee Peerapornpisal","affiliation":[],"nameIdentifiers":[]},{"name":"Park, WooJung","nameType":"Personal","givenName":"WooJung","familyName":"Park","affiliation":[],"nameIdentifiers":[]},{"name":"SangGuan You","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Structural characterization of sulfated arabinans extracted from \u003ci\u003eCladophora glomerata\u003c/i\u003e Kützing and their macrophage activation"}],"publisher":"Figshare","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"59999 Environmental Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"39999 Chemical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Plant Biology"}],"contributors":[],"dates":[{"date":"2016-01-28","dateType":"Created"},{"date":"2016-04-22","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC-BY","rightsUri":"http://creativecommons.org/licenses/by/3.0/us"}],"descriptions":[{"description":"Water-soluble sulfated heteropolysaccharides were extracted from \u003ci\u003eCladophora glomerata\u003c/i\u003e Kützing and fractionated by ion-exchange chromatography, which yielded two subfractions, F\u003csub\u003e1\u003c/sub\u003e and F\u003csub\u003e2\u003c/sub\u003e. The crude and fractionated polysaccharides (F\u003csub\u003e1\u003c/sub\u003e and F\u003csub\u003e2\u003c/sub\u003e) mostly consisted of carbohydrates (62.8–74.5%) with various amounts of proteins (9.00–17.3%) and sulfates (16.5–23.5%), including different levels of arabinose (41.7–54.4%), galactose (13.5–39.0%), glucose (0.80–10.6%), xylose (6.84–13.4%), and rhamnose (0.20–2.83%). Based on the size exclusion chromatography (SEC) profiles, the crude and fractions mainly contained one peak with shoulders having molecular weight (\u003ci\u003eM\u003c/i\u003e\u003csub\u003ew\u003c/sub\u003e) ranges of 358–1,501 × 10\u003csup\u003e3\u003c/sup\u003e. The F\u003csub\u003e1\u003c/sub\u003e fraction stimulated RAW264.7 cells to produce considerable amounts of nitric oxide and cytokines compared to the crude and F\u003csub\u003e2\u003c/sub\u003e fraction. The backbone of the most potent immunostimulating fraction (F\u003csub\u003e1\u003c/sub\u003e) was α-(1→4)-L-arabinopyranoside with galactose and xylose residues as branches at O-2 position, and sulfates mainly at O-2 position as well. Sulfated arabinans obtained from \u003ci\u003eCladophora glomerata\u003c/i\u003e Kützing were fractionated using anion-exchange chromatography to investigate structural characteristics and biological activities","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/collections/Structural_characterization_of_sulfated_arabinans_extracted_from_i_Cladophora_glomerata_i_K_tzing_and_their_macrophage_activation/2179191/1","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"api","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-28T13:10:26.000Z","registered":"2016-01-28T13:10:27.000Z","published":"2016","updated":"2024-12-14T13:47:41.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.c.2179191.v1","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}