{"data":{"id":"10.6084/m9.figshare.c.2175540","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2175540","prefix":"10.6084","suffix":"m9.figshare.c.2175540","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Lv, Qingyan","givenName":"Qingyan","familyName":"Lv","affiliation":[],"nameIdentifiers":[]},{"name":"Yuan, Lin","givenName":"Lin","familyName":"Yuan","affiliation":[],"nameIdentifiers":[]},{"name":"Song, Yuning","givenName":"Yuning","familyName":"Song","affiliation":[],"nameIdentifiers":[]},{"name":"Sui, Tingting","givenName":"Tingting","familyName":"Sui","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Zhanjun","givenName":"Zhanjun","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Lai, Liangxue","givenName":"Liangxue","familyName":"Lai","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"D-repeat in the \u003ci\u003eXIST\u003c/i\u003e gene is required for X chromosome inactivation"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Genetics"},{"subject":"Molecular Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences not elsewhere classified"},{"subject":"Science Policy"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2016-01-20","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eXIST\u003c/i\u003e is a long non-coding RNA, which expressed exclusively from the inactive X chromosome. Although it has been revealed that the A-repeat contributes to the X chromosome inactivation (X-inactivation), the role of the longest D-repeat has not yet been investigated. Here, a sgRNA directed CRISPR/Cas9 system which have multiple target sites within repeat D of \u003ci\u003eXIST\u003c/i\u003e, were used to generate D-repeat deletion and studied its roles on X-inactivation. The results showed that the deletion of D-repeat caused a significantly decreased expression of \u003ci\u003eXIST\u003c/i\u003e, and up regulated expression of X-linked genes, suggesting that the D-repeat may play an important role in the regulation of \u003ci\u003eXIST\u003c/i\u003e expression and silencing of the X-linked genes, which could provide a new idea in the molecular mechanisms of X-inactivation.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/collections/D_repeat_in_the_i_XIST_i_gene_is_required_for_X_chromosome_inactivation/2175540","contentUrl":null,"metadataVersion":4,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-20T05:54:09.000Z","registered":"2016-01-20T05:54:10.000Z","published":"2016","updated":"2024-03-21T11:24:37.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}}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