{"data":{"id":"10.6084/m9.figshare.c.2174267","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2174267","prefix":"10.6084","suffix":"m9.figshare.c.2174267","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Wang, Kai-Ling","givenName":"Kai-Ling","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Zhang, Gen","givenName":"Gen","familyName":"Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Sun, Jin","givenName":"Jin","familyName":"Sun","affiliation":[],"nameIdentifiers":[]},{"name":"Xu, Ying","givenName":"Ying","familyName":"Xu","affiliation":[],"nameIdentifiers":[]},{"name":"Han, Zhuang","givenName":"Zhuang","familyName":"Han","affiliation":[],"nameIdentifiers":[]},{"name":"Liu, Ling-Li","givenName":"Ling-Li","familyName":"Liu","affiliation":[],"nameIdentifiers":[]},{"name":"Shao, Chang-Lun","givenName":"Chang-Lun","familyName":"Shao","affiliation":[],"nameIdentifiers":[]},{"name":"Liu, Qing-Ai","givenName":"Qing-Ai","familyName":"Liu","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Chang-Yun","givenName":"Chang-Yun","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Qian, Pei-Yuan","givenName":"Pei-Yuan","familyName":"Qian","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Cochliomycin A inhibits the larval settlement of \u003ci\u003eAmphibalanus amphitrite\u003c/i\u003e by activating the NO/cGMP pathway"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Neuroscience"},{"subject":"Physiology"},{"subject":"Pharmacology"},{"subject":"Chemical Sciences not elsewhere classified"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Inorganic Chemistry"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2016-01-08","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Cochliomycin A is a compound with anti-barnacle settlement activity and low toxicity, but the molecular mechanism of the compound is unknown. Here, isobaric tags for the relative or absolute quantitation (iTRAQ) labeling proteomic method were applied to analyze changes in the proteome of \u003ci\u003eAmphibalanus\u003c/i\u003e（=\u003ci\u003eBalanus\u003c/i\u003e） \u003ci\u003eamphitrite\u003c/i\u003e cyprids in response to cochliomycin A treatment. Cochliomycin A affected the cytochrome P450, glutathione S-transferase (GST) and NO/cGMP pathways, among which the NO/cGMP pathway was considered to play a key role in barnacle larval settlement, while the cytochrome P450 and the GST pathways are mainly for detoxification. The results of real-time PCR further suggested the NO/cGMP pathway was activated in response to cochliomycin A. Larval settlement assays revealed that \u003ci\u003eS\u003c/i\u003e-methylisothiourea sulfate (SMIS) and 1\u003ci\u003eH\u003c/i\u003e-(1,2,4)oxadiazolo[4,3-a]quinoxalin-1-one (ODQ) rescued cyprids from cochliomycin A-induced inhibition of larval settlement. The findings supported the hypothesis that cochliomycin A inhibited barnacle larval settlement by stimulating the NO/cGMP pathway.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjQvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLkMuMjE3NDI2NzwvaWRlbnRpZmllcj4KICA8Y3JlYXRvcnM+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkthaS1MaW5nIFdhbmc8L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPkthaS1MaW5nPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPldhbmc8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkdlbiBaaGFuZzwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+R2VuPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPlpoYW5nPC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5KaW4gU3VuPC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5KaW48L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+U3VuPC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5ZaW5nIFh1PC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5ZaW5nPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPlh1PC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5aaHVhbmcgSGFuPC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5aaHVhbmc8L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+SGFuPC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5MaW5nLUxpIExpdTwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+TGluZy1MaTwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5MaXU8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkNoYW5nLUx1biBTaGFvPC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5DaGFuZy1MdW48L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+U2hhbzwvZmFtaWx5TmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+UWluZy1BaSBMaXU8L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPlFpbmctQWk8L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+TGl1PC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5DaGFuZy1ZdW4gV2FuZzwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+Q2hhbmctWXVuPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPldhbmc8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlBlaS1ZdWFuIFFpYW48L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPlBlaS1ZdWFuPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPlFpYW48L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgPC9jcmVhdG9ycz4KICA8dGl0bGVzPgogICAgPHRpdGxlPkNvY2hsaW9teWNpbiBBIGluaGliaXRzIHRoZSBsYXJ2YWwgc2V0dGxlbWVudCBvZiAmbHQ7aSZndDtBbXBoaWJhbGFudXMgYW1waGl0cml0ZSZsdDsvaSZndDsgYnkgYWN0aXZhdGluZyB0aGUgTk8vY0dNUCBwYXRod2F5PC90aXRsZT4KICA8L3RpdGxlcz4KICA8ZGVzY3JpcHRpb25zPgogICAgPGRlc2NyaXB0aW9uIGRlc2NyaXB0aW9uVHlwZT0iQWJzdHJhY3QiPiZsdDtwJmd0O0NvY2hsaW9teWNpbiBBIGlzIGEgY29tcG91bmQgd2l0aCBhbnRpLWJhcm5hY2xlIHNldHRsZW1lbnQgYWN0aXZpdHkgYW5kIGxvdyB0b3hpY2l0eSwgYnV0IHRoZSBtb2xlY3VsYXIgbWVjaGFuaXNtIG9mIHRoZSBjb21wb3VuZCBpcyB1bmtub3duLiBIZXJlLCBpc29iYXJpYyB0YWdzIGZvciB0aGUgcmVsYXRpdmUgb3IgYWJzb2x1dGUgcXVhbnRpdGF0aW9uIChpVFJBUSkgbGFiZWxpbmcgcHJvdGVvbWljIG1ldGhvZCB3ZXJlIGFwcGxpZWQgdG8gYW5hbHl6ZSBjaGFuZ2VzIGluIHRoZSBwcm90ZW9tZSBvZiAmbHQ7aSZndDtBbXBoaWJhbGFudXMmbHQ7L2kmZ3Q777yIPSZsdDtpJmd0O0JhbGFudXMmbHQ7L2kmZ3Q777yJICZsdDtpJmd0O2FtcGhpdHJpdGUmbHQ7L2kmZ3Q7IGN5cHJpZHMgaW4gcmVzcG9uc2UgdG8gY29jaGxpb215Y2luIEEgdHJlYXRtZW50LiBDb2NobGlvbXljaW4gQSBhZmZlY3RlZCB0aGUgY3l0b2Nocm9tZSBQNDUwLCBnbHV0YXRoaW9uZSBTLXRyYW5zZmVyYXNlIChHU1QpIGFuZCBOTy9jR01QIHBhdGh3YXlzLCBhbW9uZyB3aGljaCB0aGUgTk8vY0dNUCBwYXRod2F5IHdhcyBjb25zaWRlcmVkIHRvIHBsYXkgYSBrZXkgcm9sZSBpbiBiYXJuYWNsZSBsYXJ2YWwgc2V0dGxlbWVudCwgd2hpbGUgdGhlIGN5dG9jaHJvbWUgUDQ1MCBhbmQgdGhlIEdTVCBwYXRod2F5cyBhcmUgbWFpbmx5IGZvciBkZXRveGlmaWNhdGlvbi4gVGhlIHJlc3VsdHMgb2YgcmVhbC10aW1lIFBDUiBmdXJ0aGVyIHN1Z2dlc3RlZCB0aGUgTk8vY0dNUCBwYXRod2F5IHdhcyBhY3RpdmF0ZWQgaW4gcmVzcG9uc2UgdG8gY29jaGxpb215Y2luIEEuIExhcnZhbCBzZXR0bGVtZW50IGFzc2F5cyByZXZlYWxlZCB0aGF0ICZsdDtpJmd0O1MmbHQ7L2kmZ3Q7LW1ldGh5bGlzb3RoaW91cmVhIHN1bGZhdGUgKFNNSVMpIGFuZCAxJmx0O2kmZ3Q7SCZsdDsvaSZndDstKDEsMiw0KW94YWRpYXpvbG9bNCwzLWFdcXVpbm94YWxpbi0xLW9uZSAoT0RRKSByZXNjdWVkIGN5cHJpZHMgZnJvbSBjb2NobGlvbXljaW4gQS1pbmR1Y2VkIGluaGliaXRpb24gb2YgbGFydmFsIHNldHRsZW1lbnQuIFRoZSBmaW5kaW5ncyBzdXBwb3J0ZWQgdGhlIGh5cG90aGVzaXMgdGhhdCBjb2NobGlvbXljaW4gQSBpbmhpYml0ZWQgYmFybmFjbGUgbGFydmFsIHNldHRsZW1lbnQgYnkgc3RpbXVsYXRpbmcgdGhlIE5PL2NHTVAgcGF0aHdheS4mbHQ7L3AmZ3Q7PC9kZXNjcmlwdGlvbj4KICA8L2Rlc2NyaXB0aW9ucz4KICA8c3ViamVjdHM+CiAgICA8c3ViamVjdD5CaW9jaGVtaXN0cnk8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5HZW5ldGljczwvc3ViamVjdD4KICAgIDxzdWJqZWN0Pk5ldXJvc2NpZW5jZTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PlBoeXNpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5QaGFybWFjb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5DaGVtaWNhbCBTY2llbmNlcyBub3QgZWxzZXdoZXJlIGNsYXNzaWZpZWQ8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5JbW11bm9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+RGV2ZWxvcG1lbnRhbCBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+SW5vcmdhbmljIENoZW1pc3RyeTwvc3ViamVjdD4KICAgIDxzdWJqZWN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