{"data":{"id":"10.6084/m9.figshare.c.2173889.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2173889.v1","prefix":"10.6084","suffix":"m9.figshare.c.2173889.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jiang, Chang","givenName":"Chang","familyName":"Jiang","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Hui","givenName":"Hui","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Roy, Partha","givenName":"Partha","familyName":"Roy","affiliation":[],"nameIdentifiers":[]},{"name":"Veon, William","givenName":"William","familyName":"Veon","affiliation":[],"nameIdentifiers":[]},{"name":"R Hallows, Kenneth","givenName":"Kenneth","familyName":"R Hallows","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Epithelial morphological reversion drives Profilin-1-induced elevation of p27\u003csup\u003ekip1\u003c/sup\u003e in mesenchymal triple-negative human breast cancer cells through AMP-activated protein kinase activation"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hematology"},{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Pharmacology"},{"subject":"Molecular Biology"},{"subject":"Cell Biology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2016-01-08","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2173889","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Profilin-1 (Pfn1) is an important regulator of actin polymerization that is downregulated in human breast cancer. Previous studies have shown Pfn1 has a tumor-suppressive effect on mesenchymal-like triple-negative breast cancer cells, and Pfn1-induced growth suppression is partly mediated by upregulation of cell-cycle inhibitor p27\u003csup\u003ekip1\u003c/sup\u003e (p27). In this study, we demonstrate that Pfn1 overexpression leads to accumulation of p27 through promoting AMPK activation and AMPK-dependent phosphorylation of p27 on T198 residue, a post-translational modification that leads to increased protein stabilization of p27. This pathway is mediated by Pfn1-induced epithelial morphological reversion of mesenchymal breast cancer through cadherin-mediated restoration of adherens junctions. These findings not only elucidate a potential mechanism of how Pfn1 may inhibit proliferation of mesenchymal breast cancer cells, but also highlight a novel pathway of cadherin-mediated p27 induction and therefore cell-cycle control in cells.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/collections/Epithelial_morphological_reversion_drives_Profilin_1_induced_elevation_of_p27_sup_kip1_sup_in_mesenchymal_triple_negative_human_breast_cancer_cells_through_AMP_activated_protein_kinase_activation/2173889/1","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2016-01-08T10:32:08.000Z","registered":"2016-01-08T10:32:09.000Z","published":"2016","updated":"2024-03-21T09:35:59.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.c.2173889.v1","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}