{"data":{"id":"10.6084/m9.figshare.c.2171525","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2171525","prefix":"10.6084","suffix":"m9.figshare.c.2171525","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yu, Fujun","givenName":"Fujun","familyName":"Yu","affiliation":[],"nameIdentifiers":[]},{"name":"Lu, Zhongqiu","givenName":"Zhongqiu","familyName":"Lu","affiliation":[],"nameIdentifiers":[]},{"name":"Cai, Jing","givenName":"Jing","familyName":"Cai","affiliation":[],"nameIdentifiers":[]},{"name":"Huang, Kate","givenName":"Kate","familyName":"Huang","affiliation":[],"nameIdentifiers":[]},{"name":"Chen, Bicheng","givenName":"Bicheng","familyName":"Chen","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Guojun","givenName":"Guojun","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Dong, Peihong","givenName":"Peihong","familyName":"Dong","affiliation":[],"nameIdentifiers":[]},{"name":"Zheng, Jianjian","givenName":"Jianjian","familyName":"Zheng","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"MALAT1 functions as a competing endogenous RNA to mediate Rac1 expression by sequestering miR-101b in liver fibrosis"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biochemistry"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Chemical Sciences not elsewhere classified"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences not elsewhere classified"},{"subject":"Developmental Biology"},{"subject":"Cancer"}],"contributors":[],"dates":[{"date":"2016-01-08","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Emerging evidence shows that Metastasis-associated lung adenocarcinoma transcript 1 (MALAT1) plays a pivotal role in cell proliferation, migration, and invasion in tumors. However, the biological role and underlying mechanism of MALAT1 in liver fibrosis remains undefined. In this study, up-regulation of MALAT1 was observed in fibrotic liver tissues and in activated hepatic stellate cells (HSCs). In addition, depletion of MALAT1 inhibited the activation of HSCs \u003ci\u003ein vitro\u003c/i\u003e and attenuated collagen deposits \u003ci\u003ein vivo\u003c/i\u003e. Our results demonstrated that MALAT1 expression is negatively correlated with microRNA-101b (miR-101b) expression. Furthermore, there was a negative feedback loop between the levels of MALAT1 and miR-101b. Luciferase reporter assay indicated that MALAT1 and RAS-related C3 botulinum substrate 1 (Rac1) are targets of miR-101b. We uncovered that MALAT1 regulates Rac1 expression through miR-101b as a competing endogenous RNA (ceRNA), thereby influencing the proliferation, cell cycle and activation of primary HSCs. Collectively, The ceRNA regulatory network may prompt a better understanding of liver fibrogenesis and contribute to a novel therapeutic strategy for liver fibrosis.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjQvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLkMuMjE3MTUyNTwvaWRlbnRpZmllcj4KICA8Y3JlYXRvcnM+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkZ1anVuIFl1PC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5GdWp1bjwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5ZdTwvZmFtaWx5TmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+WmhvbmdxaXUgTHU8L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPlpob25ncWl1PC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPkx1PC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5KaW5nIENhaTwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+SmluZzwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5DYWk8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkthdGUgSHVhbmc8L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPkthdGU8L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+SHVhbmc8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkJpY2hlbmcgQ2hlbjwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+QmljaGVuZzwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5DaGVuPC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5HdW9qdW4gTGk8L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPkd1b2p1bjwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5MaTwvZmFtaWx5TmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+UGVpaG9uZyBEb25nPC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5QZWlob25nPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPkRvbmc8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkppYW5qaWFuIFpoZW5nPC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5KaWFuamlhbjwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5aaGVuZzwvZmFtaWx5TmFtZT4KICAgIDwvY3JlYXRvcj4KICA8L2NyZWF0b3JzPgogIDx0aXRsZXM+CiAgICA8dGl0bGU+TUFMQVQxIGZ1bmN0aW9ucyBhcyBhIGNvbXBldGluZyBlbmRvZ2Vub3VzIFJOQSB0byBtZWRpYXRlIFJhYzEgZXhwcmVzc2lvbiBieSBzZXF1ZXN0ZXJpbmcgbWlSLTEwMWIgaW4gbGl2ZXIgZmlicm9zaXM8L3RpdGxlPgogIDwvdGl0bGVzPgogIDxkZXNjcmlwdGlvbnM+CiAgICA8ZGVzY3JpcHRpb24gZGVzY3JpcHRpb25UeXBlPSJBYnN0cmFjdCI+Jmx0O3AmZ3Q7RW1lcmdpbmcgZXZpZGVuY2Ugc2hvd3MgdGhhdCBNZXRhc3Rhc2lzLWFzc29jaWF0ZWQgbHVuZyBhZGVub2NhcmNpbm9tYSB0cmFuc2NyaXB0IDEgKE1BTEFUMSkgcGxheXMgYSBwaXZvdGFsIHJvbGUgaW4gY2VsbCBwcm9saWZlcmF0aW9uLCBtaWdyYXRpb24sIGFuZCBpbnZhc2lvbiBpbiB0dW1vcnMuIEhvd2V2ZXIsIHRoZSBiaW9sb2dpY2FsIHJvbGUgYW5kIHVuZGVybHlpbmcgbWVjaGFuaXNtIG9mIE1BTEFUMSBpbiBsaXZlciBmaWJyb3NpcyByZW1haW5zIHVuZGVmaW5lZC4gSW4gdGhpcyBzdHVkeSwgdXAtcmVndWxhdGlvbiBvZiBNQUxBVDEgd2FzIG9ic2VydmVkIGluIGZpYnJvdGljIGxpdmVyIHRpc3N1ZXMgYW5kIGluIGFjdGl2YXRlZCBoZXBhdGljIHN0ZWxsYXRlIGNlbGxzIChIU0NzKS4gSW4gYWRkaXRpb24sIGRlcGxldGlvbiBvZiBNQUxBVDEgaW5oaWJpdGVkIHRoZSBhY3RpdmF0aW9uIG9mIEhTQ3MgJmx0O2kmZ3Q7aW4gdml0cm8mbHQ7L2kmZ3Q7IGFuZCBhdHRlbnVhdGVkIGNvbGxhZ2VuIGRlcG9zaXRzICZsdDtpJmd0O2luIHZpdm8mbHQ7L2kmZ3Q7LiBPdXIgcmVzdWx0cyBkZW1vbnN0cmF0ZWQgdGhhdCBNQUxBVDEgZXhwcmVzc2lvbiBpcyBuZWdhdGl2ZWx5IGNvcnJlbGF0ZWQgd2l0aCBtaWNyb1JOQS0xMDFiIChtaVItMTAxYikgZXhwcmVzc2lvbi4gRnVydGhlcm1vcmUsIHRoZXJlIHdhcyBhIG5lZ2F0aXZlIGZlZWRiYWNrIGxvb3AgYmV0d2VlbiB0aGUgbGV2ZWxzIG9mIE1BTEFUMSBhbmQgbWlSLTEwMWIuIEx1Y2lmZXJhc2UgcmVwb3J0ZXIgYXNzYXkgaW5kaWNhdGVkIHRoYXQgTUFMQVQxIGFuZCBSQVMtcmVsYXRlZCBDMyBib3R1bGludW0gc3Vic3RyYXRlIDEgKFJhYzEpIGFyZSB0YXJnZXRzIG9mIG1pUi0xMDFiLiBXZSB1bmNvdmVyZWQgdGhhdCBNQUxBVDEgcmVndWxhdGVzIFJhYzEgZXhwcmVzc2lvbiB0aHJvdWdoIG1pUi0xMDFiIGFzIGEgY29tcGV0aW5nIGVuZG9nZW5vdXMgUk5BIChjZVJOQSksIHRoZXJlYnkgaW5mbHVlbmNpbmcgdGhlIHByb2xpZmVyYXRpb24sIGNlbGwgY3ljbGUgYW5kIGFjdGl2YXRpb24gb2YgcHJpbWFyeSBIU0NzLiBDb2xsZWN0aXZlbHksIFRoZSBjZVJOQSByZWd1bGF0b3J5IG5ldHdvcmsgbWF5IHByb21wdCBhIGJldHRlciB1bmRlcnN0YW5kaW5nIG9mIGxpdmVyIGZpYnJvZ2VuZXNpcyBhbmQgY29udHJpYnV0ZSB0byBhIG5vdmVsIHRoZXJhcGV1dGljIHN0cmF0ZWd5IGZvciBsaXZlciBmaWJyb3Npcy4mbHQ7L3AmZ3Q7PC9kZXNjcmlwdGlvbj4KICA8L2Rlc2NyaXB0aW9ucz4KICA8c3ViamVjdHM+CiAgICA8c3ViamVjdD5CaW9jaGVtaXN0cnk8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5DZWxsIEJpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5HZW5ldGljczwvc3ViamVjdD4KICAgIDxzdWJqZWN0Pk1vbGVjdWxhciBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+Q2hlbWljYWwgU2NpZW5jZXMgbm90IGVsc2V3aGVyZSBjbGFzc2lmaWVkPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+SW1tdW5vbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkJpb2xvZ2ljYWwgU2NpZW5jZXMgbm90IGVsc2V3aGVyZSBjbGFzc2lmaWVkPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+RGV2ZWxvcG1lbnRhbCBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+Q2FuY2VyPC9zdWJqZWN0PgogIDwvc3ViamVjdHM+CiAgPHB1Ymxpc2hlcj5UYXlsb3IgJmFtcDsgRnJhbmNpczwvcHVibGlzaGVyPgogIDxwdWJsaWNhdGlvblllYXI+MjAxNjwvcHVibGljYXRpb25ZZWFyPgogIDxkYXRlcz4KICAgIDxkYXRlIGRhdGVUeXBlPSJDcmVhdGVkIj4yMDE2LTAxLTA4PC9kYXRlPgogICAgPGRhdGUgZGF0ZVR5cGU9IlVwZGF0ZWQiPjIwMTktMDgtMTk8L2RhdGU+CiAgPC9kYXRlcz4KICA8cmVzb3VyY2VUeXBlIHJlc291cmNlVHlwZUdlbm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