{"data":{"id":"10.6084/m9.figshare.c.2168381.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2168381.v1","prefix":"10.6084","suffix":"m9.figshare.c.2168381.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jiang, Tao","givenName":"Tao","familyName":"Jiang","affiliation":[],"nameIdentifiers":[]},{"name":"Huang, Mengmeng","givenName":"Mengmeng","familyName":"Huang","affiliation":[],"nameIdentifiers":[]},{"name":"He, Hao","givenName":"Hao","familyName":"He","affiliation":[],"nameIdentifiers":[]},{"name":"Lu, Jian","givenName":"Jian","familyName":"Lu","affiliation":[],"nameIdentifiers":[]},{"name":"Zhang, Yuanxing","givenName":"Yuanxing","familyName":"Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Zhou, Xiangshan","givenName":"Xiangshan","familyName":"Zhou","affiliation":[],"nameIdentifiers":[]},{"name":"Cai, Menghao","givenName":"Menghao","familyName":"Cai","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Bioprocess exploration for thermostable α-amylase production of a deep-sea thermophile \u003ci\u003eGeobacillus\u003c/i\u003e sp. in high temperature bioreactor"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Inorganic Chemistry"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Earth and Environmental Sciences"}],"contributors":[],"dates":[{"date":"2015-12-18","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2168381","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"ABSTRACT\u003ci\u003eGeobacillus\u003c/i\u003e sp. 4j, a deep-sea high-salt thermophile, was found to produce thermostable α -amylase. In this work, culture medium and conditions were firstly optimized to enhance production of thermostable α -amylase by statistical methodologies. The resulting extracellular production was increased by 5 times and reached 6.40 U/ml. Then, a high-temperature batch culture of the thermophile in a 15 L house designed bioreactor was studied. The results showed that, a relatively high dissolved oxygen (600 rpm and 15 L/min) and culture temperature of 60°C facilitated both cell growth and α -amylase production. Thus, an efficient fermentation process was established with initial medium pH of 6.0, culture temperature of 60°C and keeping dissolved oxygen above 20%. It gave a α -amylase production of 79 U/ml and productivity of 19804 U/(L · h), which were 10.8 and 208 times higher than those in shake flask, respectively. This work is useful for deep-sea high-salt thermophile culture, where efforts are lacking presently.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjQvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLkMuMjE2ODM4MS5WMTwvaWRlbnRpZmllcj4KICA8Y3JlYXRvcnM+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlRhbyBKaWFuZzwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+VGFvPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPkppYW5nPC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5NZW5nbWVuZyBIdWFuZzwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+TWVuZ21lbmc8L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+SHVhbmc8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkhhbyBIZTwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+SGFvPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPkhlPC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5KaWFuIEx1PC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5KaWFuPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPkx1PC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5ZdWFueGluZyBaaGFuZzwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+WXVhbnhpbmc8L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+Wmhhbmc8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlhpYW5nc2hhbiBaaG91PC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5YaWFuZ3NoYW48L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+WmhvdTwvZmFtaWx5TmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+TWVuZ2hhbyBDYWk8L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPk1lbmdoYW88L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+Q2FpPC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogIDwvY3JlYXRvcnM+CiAgPHRpdGxlcz4KICAgIDx0aXRsZT5CaW9wcm9jZXNzIGV4cGxvcmF0aW9uIGZvciB0aGVybW9zdGFibGUgzrEtYW15bGFzZSBwcm9kdWN0aW9uIG9mIGEgZGVlcC1zZWEgdGhlcm1vcGhpbGUgJmx0O2kmZ3Q7R2VvYmFjaWxsdXMmbHQ7L2kmZ3Q7IHNwLiBpbiBoaWdoIHRlbXBlcmF0dXJlIGJpb3JlYWN0b3I8L3RpdGxlPgogIDwvdGl0bGVzPgogIDxkZXNjcmlwdGlvbnM+CiAgICA8ZGVzY3JpcHRpb24gZGVzY3JpcHRpb25UeXBlPSJBYnN0cmFjdCI+Jmx0O2RpdiZndDsmbHQ7cCZndDtBQlNUUkFDVCZsdDsvcCZndDsmbHQ7cCZndDsmbHQ7aSZndDtHZW9iYWNpbGx1cyZsdDsvaSZndDsgc3AuIDRqLCBhIGRlZXAtc2VhIGhpZ2gtc2FsdCB0aGVybW9waGlsZSwgd2FzIGZvdW5kIHRvIHByb2R1Y2UgdGhlcm1vc3RhYmxlIM6xIC1hbXlsYXNlLiBJbiB0aGlzIHdvcmssIGN1bHR1cmUgbWVkaXVtIGFuZCBjb25kaXRpb25zIHdlcmUgZmlyc3RseSBvcHRpbWl6ZWQgdG8gZW5oYW5jZSBwcm9kdWN0aW9uIG9mIHRoZXJtb3N0YWJsZSDOsSAtYW15bGFzZSBieSBzdGF0aXN0aWNhbCBtZXRob2RvbG9naWVzLiBUaGUgcmVzdWx0aW5nIGV4dHJhY2VsbHVsYXIgcHJvZHVjdGlvbiB3YXMgaW5jcmVhc2VkIGJ5IDUgdGltZXMgYW5kIHJlYWNoZWQgNi40MCBVL21sLiBUaGVuLCBhIGhpZ2gtdGVtcGVyYXR1cmUgYmF0Y2ggY3VsdHVyZSBvZiB0aGUgdGhlcm1vcGhpbGUgaW4gYSAxNcKgTCBob3VzZSBkZXNpZ25lZCBiaW9yZWFjdG9yIHdhcyBzdHVkaWVkLiBUaGUgcmVzdWx0cyBzaG93ZWQgdGhhdCwgYSByZWxhdGl2ZWx5IGhpZ2ggZGlzc29sdmVkIG94eWdlbiAoNjAwwqBycG0gYW5kIDE1wqBML21pbikgYW5kIGN1bHR1cmUgdGVtcGVyYXR1cmUgb2YgNjDCsEMgZmFjaWxpdGF0ZWQgYm90aCBjZWxsIGdyb3d0aCBhbmQgzrEgLWFteWxhc2UgcHJvZHVjdGlvbi4gVGh1cywgYW4gZWZmaWNpZW50IGZlcm1lbnRhdGlvbiBwcm9jZXNzIHdhcyBlc3RhYmxpc2hlZCB3aXRoIGluaXRpYWwgbWVkaXVtIHBIIG9mIDYuMCwgY3VsdHVyZSB0ZW1wZXJhdHVyZSBvZiA2MMKwQyBhbmQga2VlcGluZyBkaXNzb2x2ZWQgb3h5Z2VuIGFib3ZlIDIwJS4gSXQgZ2F2ZSBhIM6xIC1hbXlsYXNlIHByb2R1Y3Rpb24gb2YgNzkgVS9tbCBhbmQgcHJvZHVjdGl2aXR5IG9mIDE5ODA0IFUvKEzCoMK3wqBoKSwgd2hpY2ggd2VyZSAxMC44IGFuZCAyMDggdGltZXMgaGlnaGVyIHRoYW4gdGhvc2UgaW4gc2hha2UgZmxhc2ssIHJlc3BlY3RpdmVseS4gVGhpcyB3b3JrIGlzIHVzZWZ1bCBmb3IgZGVlcC1zZWEgaGlnaC1zYWx0IHRoZXJtb3BoaWxlIGN1bHR1cmUsIHdoZXJlIGVmZm9ydHMgYXJlIGxhY2tpbmcgcHJlc2VudGx5LiZsdDsvcCZndDsmbHQ7L2RpdiZndDs8L2Rlc2NyaXB0aW9uPgogIDwvZGVzY3JpcHRpb25zPgogIDxzdWJqZWN0cz4KICAgIDxzdWJqZWN0Pklub3JnYW5pYyBDaGVtaXN0cnk8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW9sb2dpY2FsIFNjaWVuY2VzPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+SW1tdW5vbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkVjb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5DaGVtaXN0cnk8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5FYXJ0aCBhbmQgRW52aXJvbm1lbnRhbCBTY2llbmNlczwvc3ViamVjdD4KICA8L3N1YmplY3RzPgogIDxwdWJsaXNoZXI+VGF5bG9yICZhbXA7IEZyYW5jaXM8L3B1Ymxpc2hlcj4KICA8cHVibGljYXRpb25ZZWFyPjIwMTU8L3B1YmxpY2F0aW9uWWVhcj4KICA8ZGF0ZXM+CiAgICA8ZGF0ZSBkYXRlVHlwZT0iQ3JlYXRlZCI+MjAxNS0xMi0xODwvZGF0ZT4KICAgIDxkYXRlIGRhdGVUeXBlPSJVcGRhdGVkIj4yMDE5LTA4LTE5PC9kYXRlPgogIDwvZGF0ZXM+CiAgPHJlc291cmNlVHlwZSByZXNvdXJjZVR5cGVHZW5lcmFsPSJDb2xsZWN0aW9uIj5Db2xsZWN0aW9uPC9yZXNvdXJjZVR5cGU+CiAgPHJlbGF0ZWRJZGVudGlmaWVycz4KICAgIDxyZWxhdGVkSWRlbnRpZmllciByZWxhdGVkSWRlbnRpZmllclR5cGU9IkRPSSIgcmVsYXRpb25UeXBlPSJJc0lkZW50aWNhbFRvIj4xMC42MDg0L205LmZpZ3NoYXJlLmMuMjE2ODM4MTwvcmVsYXRlZElkZW50aWZpZXI+CiAgPC9yZWxhdGVkSWRlbnRpZmllcnM+CiAgPHJpZ2h0c0xpc3Q+CiAgICA8cmlnaHRzIHJpZ2h0c1VSST0iaHR0cHM6Ly9jcmVhdGl2ZWNvbW1vbnMub3JnL2xpY2Vuc2VzL2J5LzQuMC8iPkNDIEJZIDQuMDwvcmlnaHRzPgogIDwvcmlnaHRzTGlzdD4KPC9yZXNvdXJjZT4=","url":"https://tandf.figshare.com/c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