{"data":{"id":"10.6084/m9.figshare.c.2132558","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2132558","prefix":"10.6084","suffix":"m9.figshare.c.2132558","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Ma, Bing-cun","givenName":"Bing-cun","familyName":"Ma","affiliation":[],"nameIdentifiers":[]},{"name":"Yang, Xin","givenName":"Xin","familyName":"Yang","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Hong-ning","givenName":"Hong-ning","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Cao, Hai-peng","givenName":"Hai-peng","familyName":"Cao","affiliation":[],"nameIdentifiers":[]},{"name":"Xu, Peng-wei","givenName":"Peng-wei","familyName":"Xu","affiliation":[],"nameIdentifiers":[]},{"name":"Ding, Meng-die","givenName":"Meng-die","familyName":"Ding","affiliation":[],"nameIdentifiers":[]},{"name":"Liu, Hui","givenName":"Hui","familyName":"Liu","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Characterization of a new \u003ci\u003eLactobacillus salivarius\u003c/i\u003e strain engineered to express IBV multi-epitope antigens by chromosomal integration"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Space Science"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Genetics"},{"subject":"Biotechnology"},{"subject":"Chemical Sciences not elsewhere classified"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences not elsewhere classified"},{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"To obtain adhesive and safe lactic acid bacteria (LAB) strains for expressing heterologous antigens, we screened LAB inhabitants in intestine of Tibetan chickens by analyzing their adhesion and safety properties and the selected LAB was engineered to express heterologous antigen (UTEpi C-A) based on chromosomal integration strategy. We demonstrated that a new \u003ci\u003eLactobacillu salivarius\u003c/i\u003e TCMM17 strain is strongly adhesive to chicken intestinal epithelial cells, contains no endogenous plasmids, is susceptible to tested antimicrobials, and shows no toxicities. In order to examine the potential of TCMM17 strain as heterogenous antigen delivering vehicle, we introduced a UTEpi C-A expression cassette in its chromosome by constructing a non-replicative plasmid (pORI280-UUTEpi C-AD). The recombinant TCMM17 strain (∆TCMM17) stably was found to keep the gene cassette through 50 generations, and successfully displayed EpiC encoded by the cassette on its surface. This work provides a universal platform for development of novel oral vaccines and expression of further antigens of avian pathogens. Schematic representation of the non-replicative integration plasmid pORI280-UUTEpi C-AD construction and the overview of the homologous recombination strategy.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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