{"data":{"id":"10.6084/m9.figshare.c.2128985.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2128985.v1","prefix":"10.6084","suffix":"m9.figshare.c.2128985.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Wang, Che","givenName":"Che","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Chen, Yin-Wang","givenName":"Yin-Wang","familyName":"Chen","affiliation":[],"nameIdentifiers":[]},{"name":"Zhang, Liang","givenName":"Liang","familyName":"Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Gong, Xian-Ge","givenName":"Xian-Ge","familyName":"Gong","affiliation":[],"nameIdentifiers":[]},{"name":"Zhou, Yang","givenName":"Yang","familyName":"Zhou","affiliation":[],"nameIdentifiers":[]},{"name":"Shang, De-Jing","givenName":"De-Jing","familyName":"Shang","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Melanoma cell surface-expressed phosphatidylserine as a therapeutic target for cationic anticancer peptide, temporin-1CEa"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Pharmacology"},{"subject":"Biotechnology"},{"subject":"Chemical Sciences not elsewhere classified"},{"subject":"Astronomical and Space Sciences not elsewhere classified"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences not elsewhere classified"},{"subject":"Developmental Biology"},{"subject":"Cancer"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2128985","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"We have previously reported that temporin-1CEa, a cationic antimicrobial peptide, exerts preferential cytotoxicity toward cancer cells. However, the exact molecular mechanism for this cancer-selectivity is still largely unknown. Here, we found that the negatively charged phosphatidylserine (PS) expressed on cancer cell surface serves as a target for temporin-1CEa. Our results indicate that human A375 melanoma cells express 50-fold more PS than non-cancerous HaCaT cells. The expression of cell surface PS in various cancer cell lines closely correlated with their ability to be recognized, bound and killed by temporin-1CEa. Additionally, the cytotoxicity of temporin-1CEa against A375 cells can be ameliorated by annexin V, which binds to cell surface PS with high affinity. Moreover, the data of isothermal titration calorimetry assay further confirmed a direct binding of temporin-1CEa to PS, at a ratio of 1:5 (temporin-1CEa:PS). Interestingly, the circular dichroism spectra analysis using artificial biomembrane revealed that PS not only provides electrostatic attractive sites for temporin-1CEa but also confers the membrane-bound temporin-1CEa to form α-helical structure, therefore, enhances the affinity and membrane disrupting ability of temporin-1CEa. In summary, these findings suggested that the melanoma cells expressed PS may serve as a promising target for temporin-1CEa or other cationic anticancer peptides.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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