{"data":{"id":"10.6084/m9.figshare.c.2127836","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2127836","prefix":"10.6084","suffix":"m9.figshare.c.2127836","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Baumgardt, Kathrin","nameType":"Personal","givenName":"Kathrin","familyName":"Baumgardt","affiliation":[],"nameIdentifiers":[]},{"name":"Šmídová, Klára","nameType":"Personal","givenName":"Klára","familyName":"Šmídová","affiliation":[],"nameIdentifiers":[]},{"name":"Rahn, Helen","nameType":"Personal","givenName":"Helen","familyName":"Rahn","affiliation":[],"nameIdentifiers":[]},{"name":"Lochnit, Günter","nameType":"Personal","givenName":"Günter","familyName":"Lochnit","affiliation":[],"nameIdentifiers":[]},{"name":"Robledo, Marta","nameType":"Personal","givenName":"Marta","familyName":"Robledo","affiliation":[],"nameIdentifiers":[]},{"name":"Evguenieva-Hackenberg, Elena","nameType":"Personal","givenName":"Elena","familyName":"Evguenieva-Hackenberg","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The stress-related, rhizobial small RNA RcsR1 destabilizes the autoinducer synthase encoding mRNA \u003ci\u003esinI\u003c/i\u003e in \u003ci\u003eSinorhizobium meliloti\u003c/i\u003e"}],"publisher":"Figshare","container":{},"publicationYear":2015,"subjects":[{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Ecology"},{"subject":"Developmental Biology"},{"subject":"Mental Health"},{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2016-05-13","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC-BY","rightsUri":"http://creativecommons.org/licenses/by/3.0/us"}],"descriptions":[{"description":"Quorum sensing is a cell density-dependent communication system of bacteria relying on autoinducer molecules. During the analysis of the post-transcriptional regulation of quorum sensing in the nitrogen fixing plant symbiont \u003ci\u003eSinorhizobium meliloti,\u003c/i\u003e we predicted and verified a direct interaction between the 5'-UTR of \u003ci\u003esinI\u003c/i\u003e mRNA encoding the autoinducer synthase and a small RNA (sRNA), which we named RcsR1. \u003ci\u003eIn vitro\u003c/i\u003e, RcsR1 prevented cleavage in the 5'-UTR of \u003ci\u003esinI\u003c/i\u003e by RNase E and impaired \u003ci\u003esinI\u003c/i\u003e translation. In line with low ribosomal occupancy and transcript destabilization upon binding of RcsR1 to \u003ci\u003esinI\u003c/i\u003e, overproduction of RcsR1 in \u003ci\u003eS. meliloti\u003c/i\u003e resulted in lower level and shorter half-life of \u003ci\u003esinI\u003c/i\u003e mRNA, and in decreased autoinducer amount. Although RcsR1 can influence quorum sensing via \u003ci\u003esinI\u003c/i\u003e, its level did not vary at different cell densities, but decreased under salt stress and increased at low temperature. We found that RcsR1 and its stress-related expression pattern, but not the interaction with \u003ci\u003esinI\u003c/i\u003e homologs, are conserved in \u003ci\u003eSinorhizobium\u003c/i\u003e, \u003ci\u003eRhizobium\u003c/i\u003e and \u003ci\u003eAgrobacterium.\u003c/i\u003e Consistently, overproduction of RcsR1 in \u003ci\u003eS. meliloti\u003c/i\u003e and \u003ci\u003eAgrobacterium tumefaciens\u003c/i\u003e inhibited growth at high salinity. We identified conserved targets of RcsR1 and showed that most conserved interactions and the effect on growth under salt stress are mediated by the first stem-loop of RcsR1, while its central part is responsible for the species-specific interaction with \u003ci\u003esinI\u003c/i\u003e. We conclude that RcsR1 is an ancient, stress-related riboregulator in rhizobia and propose that it links stress responses to quorum sensing in \u003ci\u003eS. meliloti.\u003c/i\u003e","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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