{"data":{"id":"10.6084/m9.figshare.c.2127767","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2127767","prefix":"10.6084","suffix":"m9.figshare.c.2127767","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jia, Wei","givenName":"Wei","familyName":"Jia","affiliation":[],"nameIdentifiers":[]},{"name":"He, Ming-Xiao","givenName":"Ming-Xiao","familyName":"He","affiliation":[],"nameIdentifiers":[]},{"name":"X McLeod, Ian","givenName":"Ian","familyName":"X McLeod","affiliation":[],"nameIdentifiers":[]},{"name":"Guo, Jian","givenName":"Jian","familyName":"Guo","affiliation":[],"nameIdentifiers":[]},{"name":"Ji, Dong","givenName":"Dong","familyName":"Ji","affiliation":[],"nameIdentifiers":[]},{"name":"He, You-Wen","givenName":"You-Wen","familyName":"He","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Autophagy regulates T lymphocyte proliferation through selective degradation of the cell-cycle inhibitor CDKN1B/p27Kip1"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Medicine"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The highly conserved cellular degradation pathway, macroautophagy, regulates the homeostasis of organelles and promotes the survival of T lymphocytes. Previous results indicate that \u003ci\u003eAtg3-\u003c/i\u003e, \u003ci\u003eAtg5\u003c/i\u003e-, or \u003ci\u003ePik3c3/Vps34\u003c/i\u003e-deficient T cells cannot proliferate efficiently. Here we demonstrate that the proliferation of \u003ci\u003eAtg7\u003c/i\u003e-deficient T cells is defective. By using an adoptive transfer and \u003ci\u003eListeria monocytogenes\u003c/i\u003e (LM) mouse infection model, we found that the primary immune response against LM is intrinsically impaired in autophagy-deficient CD8\u003csup\u003e+\u003c/sup\u003e T cells because the cell population cannot expand after infection. Autophagy-deficient T cells fail to enter into S-phase after TCR stimulation. The major negative regulator of the cell cycle in T lymphocytes, CDKN1B, is accumulated in autophagy-deficient naïve T cells and CDKN1B cannot be degraded after TCR stimulation. Furthermore, our results indicate that genetic deletion of one allele of CDKN1B in autophagy-deficient T cells restores proliferative capability and the cells can enter into S-phase after TCR stimulation. Finally, we found that natural CDKN1B forms polymers and is physiologically associated with the autophagy receptor protein SQSTM1/p62 (sequestosome 1). Collectively, autophagy is required for maintaining the expression level of CDKN1B in naïve T cells and selectively degrades CDKN1B after TCR stimulation.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/collections/Autophagy_regulates_T_lymphocyte_proliferation_through_sele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