{"data":{"id":"10.6084/m9.figshare.c.2118998","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2118998","prefix":"10.6084","suffix":"m9.figshare.c.2118998","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Zhang, Haibo","givenName":"Haibo","familyName":"Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Jiang, Xinglin","givenName":"Xinglin","familyName":"Jiang","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Shilu","givenName":"Shilu","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Zou, Huibin","givenName":"Huibin","familyName":"Zou","affiliation":[],"nameIdentifiers":[]},{"name":"Cao, Yujin","givenName":"Yujin","familyName":"Cao","affiliation":[],"nameIdentifiers":[]},{"name":"Xian, Mo","givenName":"Mo","familyName":"Xian","affiliation":[],"nameIdentifiers":[]},{"name":"Zhou, Fengli","givenName":"Fengli","familyName":"Zhou","affiliation":[],"nameIdentifiers":[]},{"name":"Cao, Mingle","givenName":"Mingle","familyName":"Cao","affiliation":[],"nameIdentifiers":[]},{"name":"Liu, Huizhou","givenName":"Huizhou","familyName":"Liu","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Microbial production of amino acid-modified spider dragline silk protein with intensively improved mechanical properties"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hematology"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Chemistry"},{"subject":"Evolutionary Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biotechnology"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Physics"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"ABSTRACTSpider dragline silk is a remarkably strong fiber with impressive mechanical properties, which were thought to be resulted from the specific structures of the underlying proteins and their molecular size. In this study, a silk protein 11R26 from the dragline silk protein of \u003ci\u003eNephila clavipes\u003c/i\u003e was used to analyze the potential effects of the special amino acids on the function of 11R26. Three protein derivatives ZF4, ZF5 and ZF6 were obtained by site-directed mutagenesis, based on the sequence of 11R26, and among these derivatives, serine was replaced with cysteine, isoleucine and arginine, respectively. After being expressed and purified the mechanical performance of the fibers derived from the four proteins was tested. Both hardness and average elastic modulus of ZF4 fiber increased 2.2 times compared with those of 11R26. The number of disulfide bonds in ZF4 protein was 4.67 times to that of 11R26, which implied that disulfide bonds outside the poly-Ala region affect the mechanical properties of spider silk more efficiently. The results indicated that the mechanical performances of spider silk proteins with small molecular size can be enhanced by modification of the amino acids residues. Our research has not only made the feasibility for large-scale production of spider silk proteins but also provides valuable information for protein rational design.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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