{"data":{"id":"10.6084/m9.figshare.c.2118305.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2118305.v1","prefix":"10.6084","suffix":"m9.figshare.c.2118305.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Zhao, Bo","givenName":"Bo","familyName":"Zhao","affiliation":[],"nameIdentifiers":[]},{"name":"Roy, Sourav","givenName":"Sourav","familyName":"Roy","affiliation":[],"nameIdentifiers":[]},{"name":"S. Raikhel, Alexander","givenName":"Alexander","familyName":"S. Raikhel","affiliation":[],"nameIdentifiers":[]},{"name":"J. Lucas, Keira","givenName":"Keira","familyName":"J. Lucas","affiliation":[],"nameIdentifiers":[]},{"name":"L. Gervaise, Amanda","givenName":"Amanda","familyName":"L. Gervaise","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Mosquito-specific microRNA-1890 targets the juvenile hormone-regulated serine protease JHA15 in the female mosquito gut"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Inorganic Chemistry"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2118305","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Females of the hematophagous mosquito species require a vertebrate blood meal to supply amino acids and other nutrients necessary for egg development, serving as the driving force for the spread of many vector-borne diseases in humans. Blood digestion utilizes both early and late phase serine proteases (SPs) that are differentially regulated at the transcriptional and post-transcriptional level. To uncover the regulatory complexity of SPs in the female mosquito midgut, we investigated involvement of miRNAs in regulating the juvenile hormone (JH)-controlled chymotrypsin-like SP, JHA15. We identified regulatory regions complementary to the mosquito-specific miRNA, miR-1890, within the 3′ UTR of JHA15 mRNA. The level of the JHA15 transcript is highest post eclosion and drastically declines post blood meal (PBM), exhibiting an opposite trend to miR-1890 that peaks at 24 h PBM. Depletion of miR-1890 results in defects in blood digestion, ovary development and egg deposition. JHA15 mRNA and protein levels are elevated in female mosquitoes with miR-1890 inhibition. JHA15 RNA interference in the miR-1890 depletion background alleviates miR-1890 depletion phenotypes. The miR-1890 gene is activated by the 20-hydroxyecdysone pathway that involves the ecdysone receptor and the early genes, E74B and Broad Z2. Our study suggests that miR-1890 controls JHA15 mRNA stability in a stage- and tissue- specific manner.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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