{"data":{"id":"10.6084/m9.figshare.c.2113433","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2113433","prefix":"10.6084","suffix":"m9.figshare.c.2113433","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Høgh Danielsen, Pernille","givenName":"Pernille","familyName":"Høgh Danielsen","affiliation":[],"nameIdentifiers":[]},{"name":"Cao, Yi","givenName":"Yi","familyName":"Cao","affiliation":[],"nameIdentifiers":[]},{"name":"Roursgaard, Martin","givenName":"Martin","familyName":"Roursgaard","affiliation":[],"nameIdentifiers":[]},{"name":"Møller, Peter","givenName":"Peter","familyName":"Møller","affiliation":[],"nameIdentifiers":[]},{"name":"Loft, Steffen","givenName":"Steffen","familyName":"Loft","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Endothelial cell activation, oxidative stress and inflammation induced by a panel of metal-based nanomaterials"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Cancer"},{"subject":"Developmental Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Medicine"},{"subject":"Physics"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The importance of composition, size, crystal structure, charge and coating of metal-based nanomaterials (NMs) were evaluated in human umbilical vein endothelial cells (HUVECs) and/or THP-1 monocytic cells. Biomarkers of oxidative stress and inflammation were assessed because they are important in the development of cardiovascular diseases. The NMs used were five TiO\u003csub\u003e2\u003c/sub\u003e NMs with different charge, size and crystal structure, coated and uncoated ZnO NMs and Ag which were tested in a wide concentration range. There were major differences between the types of NMs; exposure to ZnO and Ag resulted in cytotoxicity and increased gene expression levels of \u003ci\u003eHMOX1\u003c/i\u003e and \u003ci\u003eIL8\u003c/i\u003e. The intracellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1(VCAM-1) expression were highest in TiO\u003csub\u003e2\u003c/sub\u003e NM-exposed cells. There was increased adhesion of THP-1 monocytic cells onto HUVECs with Ag exposure. None of the NMs increased the intracellular ROS production. There were no major effects of the coating of ZnO NMs. The TiO\u003csub\u003e2\u003c/sub\u003e NMs data on ICAM-1 and VCAM-1 expression suggested that the anatase form was more potent than the rutile form. In addition, the larger TiO\u003csub\u003e2\u003c/sub\u003e NM was more potent than the smaller for gene expression and ICAM-1 and VCAM-1 expression. The toxicological profile of cardiovascular disease-relevant biomarkers depended on composition, size and crystal structure of TiO\u003csub\u003e2\u003c/sub\u003e NMs, whereas the charge on TiO\u003csub\u003e2\u003c/sub\u003e NMs and the coating of ZnO NMs were not associated with differences in toxicological profile.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/collections/Endothelial_cell_activation_oxidative_stress_and_inflammation_induced_by_a_panel_of_metal_based_nanomaterials/2113433","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-12-05T18:25:32.000Z","registered":"2015-12-05T18:25:33.000Z","published":"2015","updated":"2024-03-21T09:25:40.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.c.2113433","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}