{"data":{"id":"10.6084/m9.figshare.c.2112887","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2112887","prefix":"10.6084","suffix":"m9.figshare.c.2112887","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Fang, Yan","givenName":"Yan","familyName":"Fang","affiliation":[],"nameIdentifiers":[]},{"name":"He, Yue","givenName":"Yue","familyName":"He","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Hua","givenName":"Hua","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Yan, Siqi","givenName":"Siqi","familyName":"Yan","affiliation":[],"nameIdentifiers":[]},{"name":"Xing, Rui","givenName":"Rui","familyName":"Xing","affiliation":[],"nameIdentifiers":[]},{"name":"Yin, Weimin","givenName":"Weimin","familyName":"Yin","affiliation":[],"nameIdentifiers":[]},{"name":"Sima, Yanghu","givenName":"Yanghu","familyName":"Sima","affiliation":[],"nameIdentifiers":[]},{"name":"Xu, Shiqing","givenName":"Shiqing","familyName":"Xu","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Hematopoiesis toxicity induced by 4-methylumbelliferon determined in an invertebrate model organism"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences not elsewhere classified"},{"subject":"Developmental Biology"},{"subject":"Inorganic Chemistry"},{"subject":"FOS: Chemical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Science Policy"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Umbelliferone has potential value as it has an inhibitory effect on tumor cells; however, its impact on an animal's circulatory system and hematopoietic function has not been reported. In this study, 4-methylumbelliferon (4-MU), an umbelliferone derivative, was used as a model drug, and its potential toxicity on hemocytes and hematopoietic organs (HOs) was investigated using an invertebrate animal model, the silkworm, \u003ci\u003eBombyx mori\u003c/i\u003e. The results showed that the level of reactive oxygen species in HOs increased when larvae (third day of the fifth instar) were orally exposed to 4 mM 4-MU for 8 min, followed by the induction of improved antioxidative metabolism of coenzymes in hemolymph. Exposure to 4-MU also significantly upregulated the expression levels of several genes in the hemolymph and fat body (a detoxification tissue similar to the liver in mammals) including antimicrobial peptide gene \u003ci\u003ececropinA\u003c/i\u003e and \u003ci\u003emoricin\u003c/i\u003e, and a phagocytosis-related gene, \u003ci\u003etetraspanin E\u003c/i\u003e, suggesting an increased antioxidant level and antimicrobial ability of the circulatory system. However, the percentage of dead hemocytes increased and hematopoiesis significantly decreased in HOs, indicating the toxic effect of 4-MU on hemocytes and hematopoiesis, despite it inducing enhanced antioxidant and antimicrobial activity in the circulatory system.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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