{"data":{"id":"10.6084/m9.figshare.c.2112527.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2112527.v1","prefix":"10.6084","suffix":"m9.figshare.c.2112527.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Xie, Xingqiao","givenName":"Xingqiao","familyName":"Xie","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Faxiang","givenName":"Faxiang","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Yuanyuan","givenName":"Yuanyuan","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Yingli","givenName":"Yingli","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Lin, Zhijie","givenName":"Zhijie","familyName":"Lin","affiliation":[],"nameIdentifiers":[]},{"name":"Cheng, Xiaofang","givenName":"Xiaofang","familyName":"Cheng","affiliation":[],"nameIdentifiers":[]},{"name":"Liu, Jianping","givenName":"Jianping","familyName":"Liu","affiliation":[],"nameIdentifiers":[]},{"name":"Chen, Changbin","givenName":"Changbin","familyName":"Chen","affiliation":[],"nameIdentifiers":[]},{"name":"Pan, Lifeng","givenName":"Lifeng","familyName":"Pan","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Molecular basis of ubiquitin recognition by the autophagy receptor CALCOCO2"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Space Science"},{"subject":"Medicine"},{"subject":"Cell Biology"},{"subject":"Molecular Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences not elsewhere classified"},{"subject":"Cancer"},{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2112527","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The autophagy receptor CALCOCO2/NDP52 functions as a bridging adaptor and plays an essential role in the selective autophagic degradation of invading pathogens by specifically recognizing ubiquitin-coated intracellular pathogens and subsequently targeting them to the autophagic machinery; thereby it is required for innate immune defense against a range of infectious pathogens in mammals. However, the mechanistic basis underlying CALCOCO2-mediated specific recognition of ubiqutinated pathogens is still unknown. Here, using biochemical and structural analyses, we demonstrated that the cargo-binding region of CALCOCO2 contains a dynamic unconventional zinc finger as well as a C\u003csub\u003e2\u003c/sub\u003eH\u003csub\u003e2\u003c/sub\u003e-type zinc-finger, and only the C\u003csub\u003e2\u003c/sub\u003eH\u003csub\u003e2\u003c/sub\u003e-type zinc finger specifically recognizes mono-ubiquitin or poly-ubiquitin chains. In addition to elucidating the specific ubiquitin recognition mechanism of CALCOCO2, the structure of the CALCOCO2 C\u003csub\u003e2\u003c/sub\u003eH\u003csub\u003e2\u003c/sub\u003e-type zinc finger in complex with mono-ubiquitin also uncovers a unique zinc finger-binding mode for ubiquitin. Our findings provide mechanistic insight into how CALCOCO2 targets ubiquitin-decorated pathogens for autophagic degradations.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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