{"data":{"id":"10.6084/m9.figshare.c.2111465","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2111465","prefix":"10.6084","suffix":"m9.figshare.c.2111465","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yan, Quan","givenName":"Quan","familyName":"Yan","affiliation":[],"nameIdentifiers":[]},{"name":"Zhu, Hong","givenName":"Hong","familyName":"Zhu","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Feng-hua","givenName":"Feng-hua","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Feng, Jing-yang","givenName":"Jing-yang","familyName":"Feng","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Wen-qiu","givenName":"Wen-qiu","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Shi, Xiang","givenName":"Xiang","familyName":"Shi","affiliation":[],"nameIdentifiers":[]},{"name":"Zhou, Yan-ping","givenName":"Yan-ping","familyName":"Zhou","affiliation":[],"nameIdentifiers":[]},{"name":"Zhang, Xi","givenName":"Xi","familyName":"Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Sun, Xiao-dong","givenName":"Xiao-dong","familyName":"Sun","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Inhibition of TRB3 Protects Photoreceptors against Endoplasmic Reticulum Stress-Induced Apoptosis after Experimental Retinal Detachment"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Medicine"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Neuroscience"},{"subject":"Physiology"},{"subject":"Chemical Sciences not elsewhere classified"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003ePurpose\u003c/i\u003e: To investigate the expression of tribbles homologue 3 (TRB3) and its regulation on endoplasmic reticulum stress (ERS)-induced photoreceptor apoptosis after retinal detachment (RD) using a rat model. \u003ci\u003eMethods\u003c/i\u003e: RD animal model was created in Wistar rats by subretinal injection of 1% sodium hyaluronate. At various time points after RD, expression of TRB3 was detected by quantitative real-time PCR and Western blotting. TRB3 protein distribution in retina was evaluated by immunohistochemistry. RNA interference was used to inhibit TRB3 expression and subretinal injection of lentivirus TRB3 shRNA (LV-TRB3-sh) was performed. The rats were then randomly divided into four groups: normal control group, RD group, vehicle + RD group and LV-TRB3-sh + RD group. The mRNA and protein level of TRB3 as well as Caspase-12 were detected. TdT-mediated fluorescein-16-dUTP nick-end labeling (TUNEL) assay was used to detect the apoptosis of retinal cells. Retinal outer nuclear layer (ONL) thickness was measured to assess retina damage in each group. \u003ci\u003eResults\u003c/i\u003e: TRB3 expression and TRB3-positive cell count were significantly increased after RD and peaked at day 3 after RD. The ratio of TUNEL-positive photoreceptors and expression of ERS-induced apoptosis marker Caspase-12 in LV-TRB3-sh + RD group were significantly reduced. The ONL thickness in LV-TRB3-sh + RD group was thicker than that both in RD group and vehicle + RD group. \u003ci\u003eConclusion\u003c/i\u003e: TRB3 expression is up-regulated in retinas after RD and knockdown of TRB3 protects photoreceptors against ERS-induced apoptosis. TRB3 may be a crucial molecule in photoreceptor apoptosis induced by ERS after RD.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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