{"data":{"id":"10.6084/m9.figshare.c.2088272","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2088272","prefix":"10.6084","suffix":"m9.figshare.c.2088272","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Li, Qian","givenName":"Qian","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Fang, Yao","givenName":"Yao","familyName":"Fang","affiliation":[],"nameIdentifiers":[]},{"name":"Zhu, Pan","givenName":"Pan","familyName":"Zhu","affiliation":[],"nameIdentifiers":[]},{"name":"Ren, Chun-Yan","givenName":"Chun-Yan","familyName":"Ren","affiliation":[],"nameIdentifiers":[]},{"name":"Chen, Hai","givenName":"Hai","familyName":"Chen","affiliation":[],"nameIdentifiers":[]},{"name":"Gu, Jiang","givenName":"Jiang","familyName":"Gu","affiliation":[],"nameIdentifiers":[]},{"name":"Jia, Yin-ping","givenName":"Yin-ping","familyName":"Jia","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Kun","givenName":"Kun","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Tong, Wen-de","givenName":"Wen-de","familyName":"Tong","affiliation":[],"nameIdentifiers":[]},{"name":"Zhang, Wei-Jun","givenName":"Wei-Jun","familyName":"Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Pan, Jing","givenName":"Jing","familyName":"Pan","affiliation":[],"nameIdentifiers":[]},{"name":"Lu, Dong-Shui","givenName":"Dong-Shui","familyName":"Lu","affiliation":[],"nameIdentifiers":[]},{"name":"Tang, Bin","givenName":"Bin","familyName":"Tang","affiliation":[],"nameIdentifiers":[]},{"name":"Mao, Xu-hu","givenName":"Xu-hu","familyName":"Mao","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"\u003ci\u003eBurkholderia pseudomallei\u003c/i\u003e survival in lung epithelial cells benefits from miRNA-mediated suppression of ATG10"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Physiology"},{"subject":"Cell Biology"},{"subject":"Medicine"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003ci\u003eBurkholderia pseudomallei\u003c/i\u003e is the causative agent of melioidosis, a disease with high mortality, which is prevalent in tropical regions of the world. A recent study shows that \u003ci\u003eB. pseudomallei\u003c/i\u003e can survive inside mammalian cells because of its ability to actively evade cell autophagy. However, the underlying mechanisms remain unclear. In the present study, based on microarray screening, we found that ATG10 was downregulated following \u003ci\u003eB. pseudomallei\u003c/i\u003e infection in A549 human lung epithelial cells. Forced expression of ATG10 accelerated the elimination of intracellular \u003ci\u003eB. pseudomallei\u003c/i\u003e by enhancing the process of autophagy. Moreover, \u003ci\u003eMIR4458, MIR4667-5p\u003c/i\u003e, and \u003ci\u003eMIR4668-5p\u003c/i\u003e were found, by microarray screening, to be upregulated in response to \u003ci\u003eB. pseudomallei\u003c/i\u003e infection. These 3 novel miRNAs, \u003ci\u003eMIR4458, MIR4667-5p\u003c/i\u003e, and \u003ci\u003eMIR4668-5p\u003c/i\u003e, targeted to the 3′-untranslated region of \u003ci\u003eATG10\u003c/i\u003e in different time-course and spatial manners. Upregulation of these miRNAs reduced the level of ATG10 and inhibited autophagy, leading to increasing survival rate of intracellular \u003ci\u003eB. pseudomallei\u003c/i\u003e. Furthermore, the increase of these miRNAs was correlated with the reduced promoter methylation status in A549 cells in response to \u003ci\u003eB. pseudomallei\u003c/i\u003e infection. Our results reveal that 3 novel miRNAs regulate autophagy-mediated elimination of \u003ci\u003eB. pseudomallei\u003c/i\u003e by targeting \u003ci\u003eATG10\u003c/i\u003e, and provide potential targets for clinical treatment.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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