{"data":{"id":"10.6084/m9.figshare.c.2086898.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2086898.v1","prefix":"10.6084","suffix":"m9.figshare.c.2086898.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yang, Fan","givenName":"Fan","familyName":"Yang","affiliation":[],"nameIdentifiers":[]},{"name":"Abdelnabby, Hazem","givenName":"Hazem","familyName":"Abdelnabby","affiliation":[],"nameIdentifiers":[]},{"name":"Xiao, Yannong","givenName":"Yannong","familyName":"Xiao","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The Zn(II)2Cys6 putative transcription factor is involved in the regulation of leucinostatin production and pathogenicity of the nematophagous fungus \u003ci\u003ePaecilomyces lilacinus\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Computational Biology"},{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Meta Science"},{"subject":"Ecology"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Microbiology"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2086898","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Zn(II)2Cys6 transcription factor genes encode transcription regulators that manage the infection potential and production of secondary metabolites such as toxins in fungi. In this study, a gene named \u003ci\u003erolP\u003c/i\u003e that encodes a putative Zn(II)2Cys6 transcription factor regulating leucinostatin production in the filamentous fungus \u003ci\u003ePaecilomyces lilacinus\u003c/i\u003e was characterized by a gene knockout approach. The deduced proprotein consists of 705 amino acids and is highly homologous to the Zn(II)2Cys6 transcription factor of the entomopathogenic fungus \u003ci\u003eMetarhizium brunneum\u003c/i\u003e. Predictive analysis of the secondary structure of the proportion showed that it has two domains. \u003ci\u003ePaecilomyces lilacinus\u003c/i\u003e can produce nematotoxins leucinostatins A and B. Deletion of \u003ci\u003erolP\u003c/i\u003e from the \u003ci\u003eP. lilacinus\u003c/i\u003e wild-type strain Pl36-1 leads to the absence of leucinostatin A, while a large increase in the leucinostatin A level was achieved in the \u003ci\u003erolP\u003c/i\u003e overexpression strain Ov-Pl36-1. During the process of nematode infection, \u003ci\u003erolP\u003c/i\u003e showed high expression levels at 48–72 h and peaked at 48 h. Bioassay tests confirmed the requirement of \u003ci\u003erolP\u003c/i\u003e for leucinostatin production. However, the root-knot second-stage juvenile-toxicity was minimized to 35.3% in Δ\u003ci\u003erolP\u003c/i\u003e; toxicity was 97.2% with Ov-Pl36-1 compared with 68.4% with the wild type strain Pl36-1. Interestingly, neither knockout nor overexpression of the \u003ci\u003erolP\u003c/i\u003e gene affected the growth or sporulation of \u003ci\u003eP. lilacinus\u003c/i\u003e. Additionally, fungal nutrition and acidic media might stimulate the \u003ci\u003erolP\u003c/i\u003e activity of the wild type strain against nematodes. These findings suggested that the \u003ci\u003erolP\u003c/i\u003e gene is required for the induction and production of leucinostatins and is considered a leucinostatin regulatory gene in \u003ci\u003eP. lilacinus\u003c/i\u003e.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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