{"data":{"id":"10.6084/m9.figshare.c.2086694.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2086694.v1","prefix":"10.6084","suffix":"m9.figshare.c.2086694.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Wang, Tao","givenName":"Tao","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Wu, Mian-Bin","givenName":"Mian-Bin","familyName":"Wu","affiliation":[],"nameIdentifiers":[]},{"name":"Chen, Zheng-Jie","givenName":"Zheng-Jie","familyName":"Chen","affiliation":[],"nameIdentifiers":[]},{"name":"Lin, Jian-Pin","givenName":"Jian-Pin","familyName":"Lin","affiliation":[],"nameIdentifiers":[]},{"name":"Yang, Li-Rong","givenName":"Li-Rong","familyName":"Yang","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Separation, determination and antifungal activity test of the products from a new \u003ci\u003eBacillus amyloliquefaciens\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Pharmacology"},{"subject":"Biotechnology"},{"subject":"Environmental Sciences not elsewhere classified"},{"subject":"Chemical Sciences not elsewhere classified"},{"subject":"Sociology"},{"subject":"FOS: Sociology","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biological Sciences not elsewhere classified"},{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2086694","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"A new \u003ci\u003eBacillus amyloliquefaciens\u003c/i\u003e named ZJU-2011 was discovered, and the culture supernatant showed a strong inhibitory effect against \u003ci\u003eCandida albicans\u003c/i\u003e. In this study, a novel method was developed to purify the antifungal compounds in high purity. The obtained products were analysed by high performance liquid chromatography and proven to be of high purity. Mass spectrometry showed that the molecular weights of the two bioactive components were 270 and 288, respectively, and their structures were determined to be bacilysin and chlorotetaine by using \u003csup\u003e1\u003c/sup\u003eH and \u003csup\u003e13\u003c/sup\u003eC nuclear magnetic resonance spectroscopy. To the best of our knowledge, this is the first time that \u003ci\u003eB. amyloliquefaciens\u003c/i\u003e has been reported to produce bacilysin and chlorotetaine simultaneously. The minimum inhibitory concentration of chlorotetaine against six common fungal pathogens were determined to be in the range of 1.8–7.8 μg/mL. Flow chart of the process of separation and purification of bacilysin and chlorotetaine.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/collections/Separation_determination_and_antifungal_activity_test_of_the_products_from_a_new_i_Bacillus_amyloliquefaciens_i_/2086694/1","contentUrl":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