{"data":{"id":"10.6084/m9.figshare.c.2055605.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2055605.v1","prefix":"10.6084","suffix":"m9.figshare.c.2055605.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Xiaoyu Xia","affiliation":[],"nameIdentifiers":[]},{"name":"Ankur Gholkar","affiliation":[],"nameIdentifiers":[]},{"name":"Senese, Silvia","nameType":"Personal","givenName":"Silvia","familyName":"Senese","affiliation":[],"nameIdentifiers":[]},{"name":"Torres, Jorge Z","nameType":"Personal","givenName":"Jorge Z","familyName":"Torres","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size"}],"publisher":"Figshare","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Pharmacology"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2016-05-24","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1026487","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2055605","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC-BY","rightsUri":"http://creativecommons.org/licenses/by/3.0/us"}],"descriptions":[{"description":"Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the underlying mechanisms have remained elusive. We show here an important role for an LCMT1-PME-1 methylation equilibrium in controlling mitotic spindle size. Depletion of LCMT1 or overexpression of PME-1 led to long spindles. In contrast, depletion of PME-1, pharmacological inhibition of PME-1 or overexpression of LCMT1 led to short spindles. Furthermore, perturbation of the LCMT1-PME-1 methylation equilibrium led to mitotic arrest, spindle assembly checkpoint activation, defective cell divisions, induction of apoptosis and reduced cell viability. Thus, we propose that the LCMT1-PME-1 methylation equilibrium is critical for regulating mitotic spindle size and thereby proper cell division.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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