{"data":{"id":"10.6084/m9.figshare.c.2055605","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2055605","prefix":"10.6084","suffix":"m9.figshare.c.2055605","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Xiaoyu Xia","affiliation":[],"nameIdentifiers":[]},{"name":"Ankur Gholkar","affiliation":[],"nameIdentifiers":[]},{"name":"Senese, Silvia","nameType":"Personal","givenName":"Silvia","familyName":"Senese","affiliation":[],"nameIdentifiers":[]},{"name":"Torres, Jorge Z","nameType":"Personal","givenName":"Jorge Z","familyName":"Torres","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"A LCMT1-PME-1 methylation equilibrium controls mitotic spindle size"}],"publisher":"Figshare","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Pharmacology"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Cancer"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2016-05-24","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2015.1026487","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC-BY","rightsUri":"http://creativecommons.org/licenses/by/3.0/us"}],"descriptions":[{"description":"Leucine carboxyl methyltransferase-1 (LCMT1) and protein phosphatase methylesterase-1 (PME-1) are essential enzymes that regulate the methylation of the protein phosphatase 2A catalytic subunit (PP2AC). LCMT1 and PME-1 have been linked to the regulation of cell growth and proliferation, but the underlying mechanisms have remained elusive. We show here an important role for an LCMT1-PME-1 methylation equilibrium in controlling mitotic spindle size. Depletion of LCMT1 or overexpression of PME-1 led to long spindles. In contrast, depletion of PME-1, pharmacological inhibition of PME-1 or overexpression of LCMT1 led to short spindles. Furthermore, perturbation of the LCMT1-PME-1 methylation equilibrium led to mitotic arrest, spindle assembly checkpoint activation, defective cell divisions, induction of apoptosis and reduced cell viability. Thus, we propose that the LCMT1-PME-1 methylation equilibrium is critical for regulating mitotic spindle size and thereby proper cell division.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/collections/A_LCMT1_PME_1_methylation_equilibrium_co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