{"data":{"id":"10.6084/m9.figshare.c.2054360","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2054360","prefix":"10.6084","suffix":"m9.figshare.c.2054360","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Prajna Rao Krishnapura","affiliation":[],"nameIdentifiers":[]},{"name":"Prasanna D. Belur","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Isolation and screening of endophytes from the rhizomes of some Zingiberaceae plants for L-asparaginase production"}],"publisher":"Figshare","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Medicine"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biotechnology"},{"subject":"59999 Environmental Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Clinical medicine","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Virology"},{"subject":"Computational Biology"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2016-04-26","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"CC-BY","rightsUri":"http://creativecommons.org/licenses/by/3.0/us"}],"descriptions":[{"description":"Endophytes are described as microorganisms that colonize the internal tissues of healthy plants without causing any disease. Endophytes isolated from medicinal plants have been attracting considerable attention due to their high biodiversity and their predicted potential to produce a plethora of novel compounds. In this study, an attempt was made to isolate endophytes from rhizomes of five medicinal plants of Zingiberaceae family, and to screen the endophytes for L-asparaginase activity. In total, 50 endophytes (14 bacteria, 22 actinomycetes, and 14 fungi) were isolated from \u003ci\u003eAlpinia galanga, Curcuma amada, Curcuma longa, Hedychium coronarium,\u003c/i\u003e and \u003ci\u003eZingiber officinale\u003c/i\u003e; of these, 31 endophytes evidenced positive for L-asparaginase production. All the L-asparaginase-positive isolates showed L-asparaginase activity in the range of 54.17–155.93 U/mL in unoptimized medium. An endophytic fungus isolated from \u003ci\u003eCurcuma amada\u003c/i\u003e, identified as \u003ci\u003eTalaromyces pinophilus\u003c/i\u003e, was used for further experiments involving studies on the effect of certain nutritional and nonnutritional factors on L-asparaginase production in submerged fermentation. \u003ci\u003eTalaromyces pinophilus\u003c/i\u003e initially gave an enzyme activity of 108.95 U/mL, but gradually reduced to 80 U/mL due to strain degeneration. Perhaps this is the first report ever on the production of L-asparaginase from endophytes isolated from medicinal plants of Zingiberaceae family.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zOnhzaT0iaHR0cDovL3d3dy53My5vcmcvMjAwMS9YTUxTY2hlbWEtaW5zdGFuY2UiIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00L21ldGFkYXRhLnhzZCI+CiAgPGlkZW50aWZpZXIgaWRlbnRpZmllclR5cGU9IkRPSSI+MTAuNjA4NC9NOS5GSUdTSEFSRS5DLjIwNTQzNjA8L2lkZW50aWZpZXI+CiAgPGNyZWF0b3JzPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5QcmFqbmEgUmFvIEtyaXNobmFwdXJhPC9jcmVhdG9yTmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+UHJhc2FubmEgRC4gQmVsdXI8L2NyZWF0b3JOYW1lPgogICAgPC9jcmVhdG9yPgogIDwvY3JlYXRvcnM+CiAgPHRpdGxlcz4KICAgIDx0aXRsZT5Jc29sYXRpb24gYW5kIHNjcmVlbmluZyBvZiBlbmRvcGh5dGVzIGZyb20gdGhlIHJoaXpvbWVzIG9mIHNvbWUgWmluZ2liZXJhY2VhZSBwbGFudHMgZm9yIEwtYXNwYXJhZ2luYXNlIHByb2R1Y3Rpb248L3RpdGxlPgogIDwvdGl0bGVzPgogIDxwdWJsaXNoZXI+Rmlnc2hhcmU8L3B1Ymxpc2hlcj4KICA8cHVibGljYXRpb25ZZWFyPjIwMTU8L3B1YmxpY2F0aW9uWWVhcj4KICA8cmVzb3VyY2VUeXBlIHJlc291cmNlVHlwZUdlbmVyYWw9IkNvbGxlY3Rpb24iPkNvbGxlY3Rpb248L3Jlc291cmNlVHlwZT4KICA8c3ViamVjdHM+CiAgICA8c3ViamVjdD5CaW9jaGVtaXN0cnk8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5NZWRpY2luZTwvc3ViamVjdD4KICAgIDxzdWJqZWN0Pk1pY3JvYmlvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0IHN1YmplY3RTY2hlbWU9IkZpZWxkcyBvZiBTY2llbmNlIGFuZCBUZWNobm9sb2d5IChGT1MpIiBzY2hlbWVVUkk9Imh0dHA6Ly93d3cub2VjZC5vcmcvc2NpZW5jZS9pbm5vLzM4MjM1MTQ3LnBkZiI+Rk9TOiBCaW9sb2dpY2FsIHNjaWVuY2VzPC9zdWJqZWN0PgogICAgPHN1YmplY3Qgc3ViamVjdFNjaGVtZT0iRmllbGRzIG9mIFNjaWVuY2UgYW5kIFRlY2hub2xvZ3kgKEZPUykiPkZPUzogQmlvbG9naWNhbCBzY2llbmNlczwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkJpb3RlY2hub2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdCBzdWJqZWN0U2NoZW1lPSJGT1IiPjU5OTk5IEVudmlyb25tZW50YWwgU2NpZW5jZXMgbm90IGVsc2V3aGVyZSBjbGFzc2lmaWVkPC9zdWJqZWN0PgogICAgPHN1YmplY3Qgc3ViamVjdFNjaGVtZT0iRmllbGRzIG9mIFNjaWVuY2UgYW5kIFRlY2hub2xvZ3kgKEZPUykiIHNjaGVtZVVSST0iaHR0cDovL3d3dy5vZWNkLm9yZy9zY2llbmNlL2lubm8vMzgyMzUxNDcucGRmIj5GT1M6IEVhcnRoIGFuZCByZWxhdGVkIGVudmlyb25tZW50YWwgc2NpZW5jZXM8L3N1YmplY3Q+CiAgICA8c3ViamVjdCBzdWJqZWN0U2NoZW1lPSJGaWVsZHMgb2YgU2NpZW5jZSBhbmQgVGVjaG5vbG9neSAoRk9TKSI+Rk9TOiBFYXJ0aCBhbmQgcmVsYXRlZCBlbnZpcm9ubWVudGFsIHNjaWVuY2VzPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+RWNvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkltbXVub2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdCBzdWJqZWN0U2NoZW1lPSJGaWVsZHMgb2YgU2NpZW5jZSBhbmQgVGVjaG5vbG9neSAoRk9TKSIgc2NoZW1lVVJJPSJodHRwOi8vd3d3Lm9lY2Qub3JnL3NjaWVuY2UvaW5uby8zODIzNTE0Ny5wZGYiPkZPUzogQ2xpbmljYWwgbWVkaWNpbmU8L3N1YmplY3Q+CiAgICA8c3ViamVjdCBzdWJqZWN0U2NoZW1lPSJGaWVsZHMgb2YgU2NpZW5jZSBhbmQgVGVjaG5vbG9neSAoRk9TKSI+Rk9TOiBDbGluaWNhbCBtZWRpY2luZTwvc3ViamVjdD4KICAgIDxzdWJqZWN0IHN1YmplY3RTY2hlbWU9IkZPUiI+Njk5OTkgQmlvbG9naWNhbCBTY2llbmNlcyBub3QgZWxzZXdoZXJlIGNsYXNzaWZpZWQ8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5WaXJvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkNvbXB1dGF0aW9uYWwgQmlvbG9neTwvc3ViamVjdD4KICA8L3N1YmplY3RzPgogIDxkYXRlcz4KICAgIDxkYXRlIGRhdGVUeXBlPSJDcmVhdGVkIj4yMDE1LTEyLTA1PC9kYXRlPgogICAgPGRhdGUgZGF0ZVR5cGU9IlVwZGF0ZWQiPjIwMTYtMDQtMjY8L2RhdGU+CiAgICA8ZGF0ZSBkYXRlVHlwZT0iSXNzdWVkIj4yMDE1PC9kYXRlPgogIDwvZGF0ZXM+CiAgPHNpemVzLz4KICA8Zm9ybWF0cy8+CiAgPHZlcnNpb24vPgogIDxyaWdodHNMaXN0PgogICAgPHJpZ2h0cyByaWdodHNVUkk9Imh0dHA6Ly9jcmVhdGl2ZWNvbW1vbnMub3JnL2xpY2Vuc2VzL2J5LzMuMC91cyI+Q0MtQlk8L3JpZ2h0cz4KICA8L3JpZ2h0c0xpc3Q+CiAgPGRlc2NyaXB0aW9ucz4KICAgIDxkZXNjcmlwdGlvbiBkZXNjcmlwdGlvblR5cGU9IkFic3RyYWN0Ij5FbmRvcGh5dGVzIGFyZSBkZXNjcmliZWQgYXMgbWljcm9vcmdhbmlzbXMgdGhhdCBjb2xvbml6ZSB0aGUgaW50ZXJuYWwgdGlzc3VlcyBvZiBoZWFsdGh5IHBsYW50cyB3aXRob3V0IGNhdXNpbmcgYW55IGRpc2Vhc2UuIEVuZG9waHl0ZXMgaXNvbGF0ZWQgZnJvbSBtZWRpY2luYWwgcGxhbnRzIGhhdmUgYmVlbiBhdHRyYWN0aW5nIGNvbnNpZGVyYWJsZSBhdHRlbnRpb24gZHVlIHRvIHRoZWlyIGhpZ2ggYmlvZGl2ZXJzaXR5IGFuZCB0aGVpciBwcmVkaWN0ZWQgcG90ZW50aWFsIHRvIHByb2R1Y2UgYSBwbGV0aG9yYSBvZiBub3ZlbCBjb21wb3VuZHMuIEluIHRoaXMgc3R1ZHksIGFuIGF0dGVtcHQgd2FzIG1hZGUgdG8gaXNvbGF0ZSBlbmRvcGh5dGVzIGZyb20gcmhpem9tZXMgb2YgZml2ZSBtZWRpY2luYWwgcGxhbnRzIG9mIFppbmdpYmVyYWNlYWUgZmFtaWx5LCBhbmQgdG8gc2NyZWVuIHRoZSBlbmRvcGh5dGVzIGZvciBMLWFzcGFyYWdpbmFzZSBhY3Rpdml0eS4gSW4gdG90YWwsIDUwIGVuZG9waHl0ZXMgKDE0IGJhY3RlcmlhLCAyMiBhY3Rpbm9teWNldGVzLCBhbmQgMTQgZnVuZ2kpIHdlcmUgaXNvbGF0ZWQgZnJvbSAmbHQ7aSZndDtBbHBpbmlhIGdhbGFuZ2EsIEN1cmN1bWEgYW1hZGEsIEN1cmN1bWEgbG9uZ2EsIEhlZHljaGl1bSBjb3JvbmFyaXVtLCZsdDsvaSZndDsgYW5kICZsdDtpJmd0O1ppbmdpYmVyIG9mZmljaW5hbGUmbHQ7L2kmZ3Q7OyBvZiB0aGVzZSwgMzEgZW5kb3BoeXRlcyBldmlkZW5jZWQgcG9zaXRpdmUgZm9yIEwtYXNwYXJhZ2luYXNlIHByb2R1Y3Rpb24uIEFsbCB0aGUgTC1hc3BhcmFnaW5hc2UtcG9zaXRpdmUgaXNvbGF0ZXMgc2hvd2VkIEwtYXNwYXJhZ2luYXNlIGFjdGl2aXR5IGluIHRoZSByYW5nZSBvZiA1NC4xN+KAkzE1NS45MyBVL21MIGluIHVub3B0aW1pemVkIG1lZGl1bS4gQW4gZW5kb3BoeXRpYyBmdW5ndXMgaXNvbGF0ZWQgZnJvbSAmbHQ7aSZndDtDdXJjdW1hIGFtYWRhJmx0Oy9pJmd0OywgaWRlbnRpZmllZCBhcyAmbHQ7aSZndDtUYWxhcm9teWNlcyBwaW5vcGhpbHVzJmx0Oy9pJmd0Oywgd2FzIHVzZWQgZm9yIGZ1cnRoZXIgZXhwZXJpbWVudHMgaW52b2x2aW5nIHN0dWRpZXMgb24gdGhlIGVmZmVjdCBvZiBjZXJ0YWluIG51dHJpdGlvbmFsIGFuZCBub25udXRyaXRp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