{"data":{"id":"10.6084/m9.figshare.c.2053262","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2053262","prefix":"10.6084","suffix":"m9.figshare.c.2053262","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Sun, Lei","givenName":"Lei","familyName":"Sun","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Heng","givenName":"Heng","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Xu, Xiaofeng","givenName":"Xiaofeng","familyName":"Xu","affiliation":[],"nameIdentifiers":[]},{"name":"Xiao, Guanxiu","givenName":"Guanxiu","familyName":"Xiao","affiliation":[],"nameIdentifiers":[]},{"name":"Luo, Chen","givenName":"Chen","familyName":"Luo","affiliation":[],"nameIdentifiers":[]},{"name":"Xiao, Guanxiu","givenName":"Guanxiu","familyName":"Xiao","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"MicroRNA-20a is essential for normal embryogenesis by targeting \u003ci\u003evsx1\u003c/i\u003e mRNA in fish"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Physics"},{"subject":"Hematology"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Medicine"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"MicroRNAs are major post-transcriptional regulators of gene expression and have essential roles in diverse developmental processes. In vertebrates, some regulatory genes play different roles at different developmental stages. These genes are initially transcribed in a wide embryonic region but restricted within distinct cell types at subsequent stages during development. Therefore, post-transcriptional regulation is required for the transition from one developmental stage to the next and the establishment of different cell identities. However, the regulation of many multiple functional genes at post-transcription level during development remains unknown. Here we show that miR-20a can target the mRNA of \u003ci\u003evsx1\u003c/i\u003e, a multiple functional gene, at the 3′-UTR and inhibit protein expression in both goldfish and zebrafish. The expression of miR-20a is initiated ubiquitously at late gastrula stage and exhibits a tissue-specific pattern in the developing retina. Inhibition of \u003ci\u003evsx1\u003c/i\u003e 3′-UTR mediated protein expression occurs when and where miR-20a is expressed. Decoying miR-20a resulted in severely impaired head, eye and trunk formation in association with excessive generation of \u003ci\u003evsx1\u003c/i\u003e marked neurons in the spinal cord and defects of somites in the mesoderm region. These results demonstrate that miR-20a is essential for normal embryogenesis by restricting Vsx1 expression in goldfish and zebrafish, and that post-transcriptional regulation is an essential mechanism for Vsx1 playing different roles in diverse developmental processes.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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