{"data":{"id":"10.6084/m9.figshare.c.2037290","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2037290","prefix":"10.6084","suffix":"m9.figshare.c.2037290","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Mori, Shigetarou","givenName":"Shigetarou","familyName":"Mori","affiliation":[],"nameIdentifiers":[]},{"name":"Kim, Hyun","givenName":"Hyun","familyName":"Kim","affiliation":[],"nameIdentifiers":[]},{"name":"Rimbara, Emiko","givenName":"Emiko","familyName":"Rimbara","affiliation":[],"nameIdentifiers":[]},{"name":"Arakawa, Yoshichika","givenName":"Yoshichika","familyName":"Arakawa","affiliation":[],"nameIdentifiers":[]},{"name":"Shibayama, Keigo","givenName":"Keigo","familyName":"Shibayama","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Roles of Ala-149 in the catalytic activity of diadenosine tetraphosphate phosphorylase from \u003ci\u003eMycobacterium tuberculosis\u003c/i\u003e H37Rv"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Virology"},{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Science Policy"},{"subject":"Biological Sciences"},{"subject":"Earth and Environmental Sciences"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Diadenosine 5′,5′′′-\u003ci\u003eP\u003c/i\u003e\u003csup\u003e1\u003c/sup\u003e,\u003ci\u003eP\u003c/i\u003e\u003csup\u003e4\u003c/sup\u003e-tetraphosphate (Ap\u003csub\u003e4\u003c/sub\u003eA) phosphorylase from \u003ci\u003eMycobacterium tuberculosis\u003c/i\u003e H37Rv (MtAPA) belongs to the histidine triad motif (HIT) superfamily, but is the only member with an alanine residue at position 149 (Ala-149). Enzymatic analysis revealed that the Ala-149 deletion mutant displayed substrate specificity for diadenosine 5′,5′′′-\u003ci\u003eP\u003c/i\u003e\u003csup\u003e1\u003c/sup\u003e,\u003ci\u003eP\u003c/i\u003e\u003csup\u003e5\u003c/sup\u003e-pentaphosphate and was inactive on Ap\u003csub\u003e4\u003c/sub\u003eA and other substrates that are utilized by the wild-type enzyme.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/collections/Roles_of_Ala_149_in_the_catalytic_activity_of_diadenosine_tetraphosphate_phosphorylase_from_i_Mycobacterium_tuberculosis_i_H37Rv/2037290","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-12-05T17:20:36.000Z","registered":"2015-12-05T17:20:37.000Z","published":"2015","updated":"2024-03-21T08:38:38.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.c.2037290","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}