{"data":{"id":"10.6084/m9.figshare.c.2035109","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2035109","prefix":"10.6084","suffix":"m9.figshare.c.2035109","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Kang, Shuai","givenName":"Shuai","familyName":"Kang","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Zhengwen","givenName":"Zhengwen","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Yin, Zhongqiong","givenName":"Zhongqiong","familyName":"Yin","affiliation":[],"nameIdentifiers":[]},{"name":"Jia, Renyong","givenName":"Renyong","familyName":"Jia","affiliation":[],"nameIdentifiers":[]},{"name":"Song, Xu","givenName":"Xu","familyName":"Song","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Li","givenName":"Li","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Chen, Zhenzhen","givenName":"Zhenzhen","familyName":"Chen","affiliation":[],"nameIdentifiers":[]},{"name":"Peng, Lianci","givenName":"Lianci","familyName":"Peng","affiliation":[],"nameIdentifiers":[]},{"name":"Qu, Jing","givenName":"Jing","familyName":"Qu","affiliation":[],"nameIdentifiers":[]},{"name":"Hu, Zhiqiang","givenName":"Zhiqiang","familyName":"Hu","affiliation":[],"nameIdentifiers":[]},{"name":"Lai, Xin","givenName":"Xin","familyName":"Lai","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Guangxi","givenName":"Guangxi","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Liang, Xiaoxia","givenName":"Xiaoxia","familyName":"Liang","affiliation":[],"nameIdentifiers":[]},{"name":"He, Changliang","givenName":"Changliang","familyName":"He","affiliation":[],"nameIdentifiers":[]},{"name":"Yin, lizi","givenName":"lizi","familyName":"Yin","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"The antibacterial mechanism of berberine against \u003ci\u003eActinobacillus pleuropneumoniae\u003c/i\u003e"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Biotechnology"},{"subject":"Molecular Biology"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"This study demonstrated berberine to be a potential natural compound against \u003ci\u003eActinobacillus pleuropneumoniae\u003c/i\u003e. Liquid doubling dilution, transmission electron microscopy (TEM), SDS-PAGE and 4′,6-diamidino-2-phenylindole (DAPI) staining were employed to elucidate the antibacterial activity and mechanism of berberine. The minimal inhibitory concentration of berberine was 0.3125 mg/mL, and time–kill curves showed concentration and time dependence. The TEM micrographs displayed damaged cell wall, concentrated cytoplasm, cytoplasmic content leakage and cell death. SDS-PAGE and DAPI assays revealed that berberine can restrain DNA and protein syntheses. Berberine inhibited the synthesis of proteins associated with the growth and cleavage of bacteria and then blocked the division and development of bacteria. The compound ultimately induced cytoplasm pyknosis and bacterial death.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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