{"data":{"id":"10.6084/m9.figshare.c.2034173.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2034173.v1","prefix":"10.6084","suffix":"m9.figshare.c.2034173.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Yu, Jinghua","givenName":"Jinghua","familyName":"Yu","affiliation":[],"nameIdentifiers":[]},{"name":"Zhang, Liying","givenName":"Liying","familyName":"Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Ren, Peiyou","givenName":"Peiyou","familyName":"Ren","affiliation":[],"nameIdentifiers":[]},{"name":"Zhong, Ting","givenName":"Ting","familyName":"Zhong","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Zhaolong","givenName":"Zhaolong","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Zengyan","givenName":"Zengyan","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Jingliang","givenName":"Jingliang","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Liu, Xin","givenName":"Xin","familyName":"Liu","affiliation":[],"nameIdentifiers":[]},{"name":"Zhao, Ke","givenName":"Ke","familyName":"Zhao","affiliation":[],"nameIdentifiers":[]},{"name":"Zhang, Wenyan","givenName":"Wenyan","familyName":"Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Yu, Xiao-Fang","givenName":"Xiao-Fang","familyName":"Yu","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Enterovirus 71 mediates cell cycle arrest in S phase through non-structural protein 3D"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Virology"},{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Hematology"},{"subject":"Cancer"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biotechnology"},{"subject":"Molecular Biology"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2034173","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Many viruses disrupt the host cell cycle to facilitate their own growth. We assessed the mechanism and function of enterovirus 71 (EV71), a primary causative agent for recent hand, foot, and mouth disease outbreaks, in manipulating cell cycle progression. Our results suggest that EV71 infection induces S-phase arrest in diverse cell types by preventing the cell cycle transition from the S phase into the G2/M phase. Similar results were observed for an alternate \u003ci\u003epicornavirus,\u003c/i\u003e Coxsackievirus A16. Synchronization in S phase, but not G0/G1 phase or G2/M phase, promotes viral replication. Consistent with its ability to arrest cells in S phase, the expression of cyclin A2, CDK 2, cyclin E1, and cyclin B1 was regulated by EV71 through increasing transcription of cyclin E1, promoting proteasome-mediated degradation of cyclin A2 and regulating the phosphorylation of CDK 2. Finally, a non-structural protein of EV71, the RNA-dependent RNA polymerase 3D, was demonstrated to mediate S-phase cell cycle arrest. These findings suggest that EV71 induces S-phase cell cycle arrest in infected cells via non-structural protein 3D, which may provide favorable conditions for virus production.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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