{"data":{"id":"10.6084/m9.figshare.c.2031584","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2031584","prefix":"10.6084","suffix":"m9.figshare.c.2031584","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Wang, Zhenda","givenName":"Zhenda","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Hu, Jia","givenName":"Jia","familyName":"Hu","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Ge","givenName":"Ge","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Qu, Liujing","givenName":"Liujing","familyName":"Qu","affiliation":[],"nameIdentifiers":[]},{"name":"He, Qihua","givenName":"Qihua","familyName":"He","affiliation":[],"nameIdentifiers":[]},{"name":"Lou, Yaxin","givenName":"Yaxin","familyName":"Lou","affiliation":[],"nameIdentifiers":[]},{"name":"Song, Quansheng","givenName":"Quansheng","familyName":"Song","affiliation":[],"nameIdentifiers":[]},{"name":"Ma, Dalong","givenName":"Dalong","familyName":"Ma","affiliation":[],"nameIdentifiers":[]},{"name":"Chen, Yingyu","givenName":"Yingyu","familyName":"Chen","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Biochemistry"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cancer"},{"subject":"Science Policy"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Autophagy is a multistep process that involves the degradation and digestion of intracellular components by the lysosome. It has been proved that many core autophagy-related molecules participate in this event. However, new component proteins that regulate autophagy are still being discovered. At present, we report PHF23 (PHD finger protein 23) with a PHD-like zinc finger domain that can negatively regulate autophagy. Data from experiments indicated that the overexpression of PHF23 impaired autophagy, as characterized by decreased levels of LC3B-II and weakened degradation of endogenous and exogenous autophagic substrates. Conversely, knockdown of \u003ci\u003ePHF23\u003c/i\u003e resulted in opposite effects. Molecular mechanism studies suggested that PHF23 interacts with LRSAM1, which is an E3 ligase key for ubiquitin-dependent autophagy against invading bacteria. PHF23 promotes the ubiquitination and proteasome degradation of LRSAM1. We also show that the PHD finger of PHF23 is a functional domain needed for the interaction with LRSAM1. Altogether, our results indicate that PHF23 is a negative regulator associated in autophagy via the LRSAM1 signaling pathway. The physical and functional connection between the PHF23 and LRSAM1 needs further investigation.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/collections/PHF23_plant_homeodomain_finger_protein_23_negatively_regulates_cell_autophagy_by_promoting_ubiquitination_and_degradation_of_E3_ligase_LRSAM1/2031584","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-12-05T17:09:35.000Z","registered":"2015-12-05T17:09:36.000Z","published":"2015","updated":"2024-03-21T08:36:04.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.c.2031584","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}