{"data":{"id":"10.6084/m9.figshare.c.2031020.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2031020.v1","prefix":"10.6084","suffix":"m9.figshare.c.2031020.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Ling, Yun","givenName":"Yun","familyName":"Ling","affiliation":[],"nameIdentifiers":[]},{"name":"Fu, Zhiwen","givenName":"Zhiwen","familyName":"Fu","affiliation":[],"nameIdentifiers":[]},{"name":"Zhang, Qing","givenName":"Qing","familyName":"Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Xu, Lingling","givenName":"Lingling","familyName":"Xu","affiliation":[],"nameIdentifiers":[]},{"name":"Liao, Liang","givenName":"Liang","familyName":"Liao","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Identification and structural elucidation of steroidal saponins from the root of \u003ci\u003eParis polyphylla\u003c/i\u003e by HPLC-ESI-QTOF-MS/MS"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Science Policy"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Earth and Environmental Sciences"},{"subject":"Biotechnology"},{"subject":"Pharmacology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2031020","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"The root of \u003ci\u003eParis polyphylla\u003c/i\u003e (RPP) is widely used as a traditional Chinese medicine for a long time due to the good properties of heat-clearing and detoxicating, detumescence, sedation, acesodyne and haemostasis. To clarify on the bioactive substances and ensure the safety in clinical medication, a feasible and accurate strategy was developed by applying the high-performance liquid chromatography coupled to electrospray ionisation and quadrupole time-of-flight-mass spectrometry (HPLC-ESI-QTOF-MS/MS). Separation was performed an Agilent poroshell 120 EC-C18 column (2.7 × 100 mm, i.d., 2.7 μm) with 0.1% formic acid aqueous solution and acetonitrile as the mobile phase under gradient conditions. Based on the proposed strategy, 30 constituents, mainly including steroidal saponins, were characterised or tentatively identified, 2 of which were the first to be reported as the potential new steroidal saponins in RPP. In conclusion, the HPLC-ESI-QTOF-MS/MS is a feasible and credible technique to separate and identify steroidal saponins from botanical extracts.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://tandf.figshare.com/collections/Identification_and_structural_elucidation_of_steroidal_saponins_from_the_root_of_i_Paris_polyphylla_i_by_HPLC_ESI_QTOF_MS_MS/2031020/1","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActi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