{"data":{"id":"10.6084/m9.figshare.c.2030096.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2030096.v1","prefix":"10.6084","suffix":"m9.figshare.c.2030096.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Han, Caixia","givenName":"Caixia","familyName":"Han","affiliation":[],"nameIdentifiers":[]},{"name":"Lu, Xaiobing","givenName":"Xaiobing","familyName":"Lu","affiliation":[],"nameIdentifiers":[]},{"name":"Yu, Zitong","givenName":"Zitong","familyName":"Yu","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Xiaohui","givenName":"Xiaohui","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Ma, Wujun","givenName":"Wujun","familyName":"Ma","affiliation":[],"nameIdentifiers":[]},{"name":"Yan, Yueming","givenName":"Yueming","familyName":"Yan","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Rapid separation of seed gliadins by reversed-phase ultra performance liquid chromatography (RP-UPLC) and its application in wheat cultivar and germplasm identification"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Science Policy"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Sociology"},{"subject":"FOS: Sociology","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Earth and Environmental Sciences"},{"subject":"Evolutionary Biology"},{"subject":"Biotechnology"},{"subject":"Engineering"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2030096","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"To separate gliadin from wheat flour, a novel and stability-indicating reversed-phase ultra performance liquid chromatography (RP-UPLC) method is established and optimized. A comparative analysis of routine capillary electrophoresis (CE), reversed-phase high-performance liquid chromatography (RP-HPLC), and RP-UPLC was performed and the results showed that the resolution and efficiency of RP-UPLC were significantly higher than those of CE and RP-HPLC. Characteristic RP-UPLC patterns of different bread wheat variety and related species were readily identified. These results demonstrated that our RP-UPLC procedure resulted in significant improvements in sensitivity, speed, and resolution, and thus is highly useful in wheat cultivar and germplasm identification.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjQvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLkMuMjAzMDA5Ni5WMTwvaWRlbnRpZmllcj4KICA8Y3JlYXRvcnM+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkNhaXhpYSBIYW48L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPkNhaXhpYTwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5IYW48L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlhhaW9iaW5nIEx1PC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5YYWlvYmluZzwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5MdTwvZmFtaWx5TmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+Wml0b25nIFl1PC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5aaXRvbmc8L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+WXU8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlhpYW9odWkgTGk8L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPlhpYW9odWk8L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+TGk8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPld1anVuIE1hPC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5XdWp1bjwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5NYTwvZmFtaWx5TmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+WXVlbWluZyBZYW48L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPll1ZW1pbmc8L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+WWFuPC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogIDwvY3JlYXRvcnM+CiAgPHRpdGxlcz4KICAgIDx0aXRsZT5SYXBpZCBzZXBhcmF0aW9uIG9mIHNlZWQgZ2xpYWRpbnMgYnkgcmV2ZXJzZWQtcGhhc2UgdWx0cmEgcGVyZm9ybWFuY2UgbGlxdWlkIGNocm9tYXRvZ3JhcGh5IChSUC1VUExDKSBhbmQgaXRzIGFwcGxpY2F0aW9uIGluIHdoZWF0IGN1bHRpdmFyIGFuZCBnZXJtcGxhc20gaWRlbnRpZmljYXRpb248L3RpdGxlPgogIDwvdGl0bGVzPgogIDxkZXNjcmlwdGlvbnM+CiAgICA8ZGVzY3JpcHRpb24gZGVzY3JpcHRpb25UeXBlPSJBYnN0cmFjdCI+Jmx0O2RpdiZndDsmbHQ7cCZndDtUbyBzZXBhcmF0ZSBnbGlhZGluIGZyb20gd2hlYXQgZmxvdXIsIGEgbm92ZWwgYW5kIHN0YWJpbGl0eS1pbmRpY2F0aW5nIHJldmVyc2VkLXBoYXNlIHVsdHJhIHBlcmZvcm1hbmNlIGxpcXVpZCBjaHJvbWF0b2dyYXBoeSAoUlAtVVBMQykgbWV0aG9kIGlzIGVzdGFibGlzaGVkIGFuZCBvcHRpbWl6ZWQuIEEgY29tcGFyYXRpdmUgYW5hbHlzaXMgb2Ygcm91dGluZSBjYXBpbGxhcnkgZWxlY3Ryb3Bob3Jlc2lzIChDRSksIHJldmVyc2VkLXBoYXNlIGhpZ2gtcGVyZm9ybWFuY2UgbGlxdWlkIGNocm9tYXRvZ3JhcGh5IChSUC1IUExDKSwgYW5kIFJQLVVQTEMgd2FzIHBlcmZvcm1lZCBhbmQgdGhlIHJlc3VsdHMgc2hvd2VkIHRoYXQgdGhlIHJlc29sdXRpb24gYW5kIGVmZmljaWVuY3kgb2YgUlAtVVBMQyB3ZXJlIHNpZ25pZmljYW50bHkgaGlnaGVyIHRoYW4gdGhvc2Ugb2YgQ0UgYW5kIFJQLUhQTEMuIENoYXJhY3RlcmlzdGljIFJQLVVQTEMgcGF0dGVybnMgb2YgZGlmZmVyZW50IGJyZWFkIHdoZWF0IHZhcmlldHkgYW5kIHJlbGF0ZWQgc3BlY2llcyB3ZXJlIHJlYWRpbHkgaWRlbnRpZmllZC4gVGhlc2UgcmVzdWx0cyBkZW1vbnN0cmF0ZWQgdGhhdCBvdXIgUlAtVVBMQyBwcm9jZWR1cmUgcmVzdWx0ZWQgaW4gc2lnbmlmaWNhbnQgaW1wcm92ZW1lbnRzIGluIHNlbnNpdGl2aXR5LCBzcGVlZCwgYW5kIHJlc29sdXRpb24sIGFuZCB0aHVzIGlzIGhpZ2hseSB1c2VmdWwgaW4gd2hlYXQgY3VsdGl2YXIgYW5kIGdlcm1wbGFzbSBpZGVudGlmaWNhdGlvbi4mbHQ7L3AmZ3Q7Jmx0Oy9kaXYmZ3Q7PC9kZXNjcmlwdGlvbj4KICA8L2Rlc2NyaXB0aW9ucz4KICA8c3ViamVjdHM+CiAgICA8c3ViamVjdD5QbGFudCBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+U2NpZW5jZSBQb2xpY3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW9sb2dpY2FsIFNjaWVuY2VzPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+SW1tdW5vbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PlNvY2lvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkNoZW1pc3RyeTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkVhcnRoIGFuZCBFbnZpcm9ubWVudGFsIFNjaWVuY2VzPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+RXZvbHV0aW9uYXJ5IEJpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW90ZWNobm9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+RW5naW5lZXJpbmc8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW9jaGVtaXN0cnk8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW9waHlzaWNzPC9zdWJqZWN0PgogIDwvc3ViamVjdHM+CiAgPHB1Ymxpc2hlcj5UYXlsb3IgJmFtcDsgRnJhbmNpczwvcHVibGlzaGVyPgogIDxwdWJsaWNhdGlvblllYXI+MjAxNTwvcHVibGljYXRpb25ZZWFyPgogIDxkYXRlcz4KICAgIDxkYXRlIGRhdGVUeXBlPSJDcmVhdGVkIj4yMDE1LTEyLTA1PC9kYXRlPgogICAgPGRhdGUgZGF0ZVR5cGU9IlVwZGF0ZWQiPjIwMTktMDgtMTk8L2RhdGU+CiAgPC9kYXRlcz4KICA8cmVzb3VyY2VUeXBlIHJlc291cmNlVHlwZUdlbmVyYWw9IkNvbGxlY3Rpb24iPkNvbGxlY3Rpb248L3Jlc291cmNlVHlwZT4KICA8cmVsYXRlZElkZW50aWZpZXJzPgogICAgPHJlbGF0ZWRJZGVudGlmaWVyIHJlbGF0ZWRJZGVudGlmaWVyVHlwZT0iRE9JIiByZWxhdGlvblR5cGU9IklzSWRlbnRpY2FsVG8iPjEwLjYwODQvbTkuZmlnc2hhcmUuYy4yMDMwMDk2PC9yZWxhdGVkSWRlbnRpZmllcj4KICA8L3JlbGF0ZWRJZGVudGlmaWVycz4KICA8cmlnaHRzTGlzdD4KICAgIDxyaWdodHMgcmlnaHRzVVJJPSJodHRwczovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnkvNC4wLyI+Q0MgQlkgNC4wPC9yaWdodHM+CiAgPC9yaWdodHNMaXN0Pgo8L3Jlc291cmNlPg==","url":"https://tandf.figshare.com/collections/Rapid_separation_of_seed_gliadins_by_reversed_phase_ultra_performance_liquid_chromatography_RP_UPLC_and_its_application_in_wheat_cultivar_and_germplasm_identification/2030096/1","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":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