{"data":{"id":"10.6084/m9.figshare.c.2029745.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2029745.v1","prefix":"10.6084","suffix":"m9.figshare.c.2029745.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Gu, Wenwen","givenName":"Wenwen","familyName":"Gu","affiliation":[],"nameIdentifiers":[]},{"name":"Yang, Long","givenName":"Long","familyName":"Yang","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Shuangjie","givenName":"Shuangjie","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Jiang, Lihua","givenName":"Lihua","familyName":"Jiang","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Generation and Application of a Novel InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e Mono-Antibody from Mouse"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Hematology"},{"subject":"Cardiology"},{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Biotechnology"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Neuroscience"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"Cell Biology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2029745","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Inositol 1, 4, 5-Trisphosphate Receptor (InsP\u003csub\u003e3\u003c/sub\u003eR) is an intracellular Ca\u003csup\u003e2+\u003c/sup\u003e release channel, which widely participates in cellular processes. Three isoforms of InsP\u003csub\u003e3\u003c/sub\u003eR were identified as InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e, InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e2,\u003c/sub\u003e and InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e3\u003c/sub\u003e. They share 60–0% protein sequence homology and form a channel in a manner of homotetramer or heterotetramer. Several InsP\u003csub\u003e3\u003c/sub\u003eR isoform-specific rabbit antibodies have been generated to distinguish their localization and functions. However, there is no report of such a valid antibody raised from other species. In his article, we prepare a mouse monoclonal antibody against a synthetic peptide with rat InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e-specific carboxyl terminus sequence. This monoclonal antibody of InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e (R1-mAb) was purified and characterized as IgG2b, which can recognize InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e by Western-blot (WB) analysis and immunoprecipitate (IP) InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e from moue brain lysate tested. Applied in immunofluorescent (IF) and immunohistochemical (IHC) assays, this antibody and rabbit polyclonal antibody could give the consistent results in SH-SY5Y cells, human sperm, and mouse brain paraffin section. In summary, we generate a mouse InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e-specific IgG 2b antibody identifying InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e in WB, IF, IHC, and IP analysis, which provides a possible choice for detection of InsP\u003csub\u003e3\u003c/sub\u003eR\u003csub\u003e1\u003c/sub\u003e, especially in application of co-localization analysis with other InsP\u003csub\u003e3\u003c/sub\u003eR isoforms or other proteins.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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