{"data":{"id":"10.6084/m9.figshare.c.2029061.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2029061.v1","prefix":"10.6084","suffix":"m9.figshare.c.2029061.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Sun, Tieying","givenName":"Tieying","familyName":"Sun","affiliation":[],"nameIdentifiers":[]},{"name":"Qin, Biaojie","givenName":"Biaojie","familyName":"Qin","affiliation":[],"nameIdentifiers":[]},{"name":"Gao, Mingchuan","givenName":"Mingchuan","familyName":"Gao","affiliation":[],"nameIdentifiers":[]},{"name":"Yin, Yuling","givenName":"Yuling","familyName":"Yin","affiliation":[],"nameIdentifiers":[]},{"name":"Wang, Changyuan","givenName":"Changyuan","familyName":"Wang","affiliation":[],"nameIdentifiers":[]},{"name":"Zang, Shizhu","givenName":"Shizhu","familyName":"Zang","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Xinli","givenName":"Xinli","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Zhang, Cuili","givenName":"Cuili","familyName":"Zhang","affiliation":[],"nameIdentifiers":[]},{"name":"Xin, Yi","givenName":"Yi","familyName":"Xin","affiliation":[],"nameIdentifiers":[]},{"name":"Jiang, Tao","givenName":"Tao","familyName":"Jiang","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Effects of epigallocatechin gallate on the cell-wall structure of \u003ci\u003eMycobacterial smegmatis\u003c/i\u003e mc\u003csup\u003e2\u003c/sup\u003e155"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Plant Biology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Infectious Diseases"},{"subject":"FOS: Health sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Chemistry"},{"subject":"Earth and Environmental Sciences"},{"subject":"Biotechnology"},{"subject":"Pharmacology"},{"subject":"Molecular Biology"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2029061","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Epigallocatechin gallate (EGCG) is the main component of green tea extracts that inhibits the growth of \u003ci\u003eMycobacterial smegmatis\u003c/i\u003e mc\u003csup\u003e2\u003c/sup\u003e155, and the mechanism is not clear. This study showed the effects of EGCG on the growth of mc\u003csup\u003e2\u003c/sup\u003e155. The content and the structure of EGCG in LB medium with mc\u003csup\u003e2\u003c/sup\u003e155 were identified by HPLC and LC/MS. Transmission electron microscopy was utilised to identify the cell envelope structure. As a result, the optional inhibition concentration was determined to be 20 μg mL\u003csup\u003e− 1\u003c/sup\u003e. Most of EGCG was transferred into its isomeride in LB medium, but the inhibition effects against mc\u003csup\u003e2\u003c/sup\u003e155 had yet been maintained. The changes of cell envelope structure were showed after EGCG treatment for 18 h. The cell wall appeared to have a less electron-translucent zone, turn rougher and thicker. The results show that EGCG impacts the integrity of mycobacterial cell wall and is likely be a better prophylactic agent against tuberculosis.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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