{"data":{"id":"10.6084/m9.figshare.c.2028464.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2028464.v1","prefix":"10.6084","suffix":"m9.figshare.c.2028464.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Huang, Jin","givenName":"Jin","familyName":"Huang","affiliation":[],"nameIdentifiers":[]},{"name":"Wan, Danyang","givenName":"Danyang","familyName":"Wan","affiliation":[],"nameIdentifiers":[]},{"name":"Li, Jianshuang","givenName":"Jianshuang","familyName":"Li","affiliation":[],"nameIdentifiers":[]},{"name":"Chen, Hong","givenName":"Hong","familyName":"Chen","affiliation":[],"nameIdentifiers":[]},{"name":"Huang, Kun","givenName":"Kun","familyName":"Huang","affiliation":[],"nameIdentifiers":[]},{"name":"Zheng, Ling","givenName":"Ling","familyName":"Zheng","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Histone acetyltransferase PCAF regulates inflammatory molecules in the development of renal injury"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Hematology"},{"subject":"Marine Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Chemistry"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2028464","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Kidney diseases, including chronic kidney disease (CKD) and acute kidney injury (AKI), are associated with inflammation. The mechanism that regulates inflammation in these renal injuries remains unclear. Here, we demonstrated that p300/CBP-associated factor (PCAF), a histone acetyltransferase, was overexpressed in the kidneys of db/db mice and lipopolysaccharide (LPS)-injected mice. Moreover, elevated histone acetylation, such as H3K18ac, and up-regulation of some inflammatory genes, such as \u003ci\u003eICAM-1, VCAM-1,\u003c/i\u003e and \u003ci\u003eMCP-1,\u003c/i\u003e were found upon these renal injuries. Furthermore, increased H3K18ac was recruited to the promoters of \u003ci\u003eICAM-1, VCAM-1,\u003c/i\u003e and \u003ci\u003eMCP-1\u003c/i\u003e in the kidneys of LPS-injected mice. \u003ci\u003eIn vitro\u003c/i\u003e studies demonstrated that PCAF knockdown in human renal proximal tubule epithelial cells (HK-2) led to downregulation of inflammatory molecules, including VCAM-1, ICAM-1, p50 subunit of NF-κB (p50), and MCP-1 mRNA and protein levels, together with significantly decreased H3K18ac level. Consistent with these, overexpression of PCAF enhanced the expression of inflammatory molecules. Furthermore, PCAF deficiency reduced palmitate-induced recruitment of H3K18ac on the promoters of \u003ci\u003eICAM-1\u003c/i\u003e and \u003ci\u003eMCP-1\u003c/i\u003e, as well as inhibited palmitate-induced upregulation of these inflammatory molecules. In summary, the present work demonstrates that PCAF plays an essential role in the regulation of inflammatory molecules through H3K18ac, which provides a potential therapeutic target for inflammation-related renal diseases.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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