{"data":{"id":"10.6084/m9.figshare.c.2026676.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.c.2026676.v1","prefix":"10.6084","suffix":"m9.figshare.c.2026676.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jun Lim, Su","givenName":"Su","familyName":"Jun Lim","affiliation":[],"nameIdentifiers":[]},{"name":"Scott, Anthony","givenName":"Anthony","familyName":"Scott","affiliation":[],"nameIdentifiers":[]},{"name":"Xiong, Xiao-Peng","givenName":"Xiao-Peng","familyName":"Xiong","affiliation":[],"nameIdentifiers":[]},{"name":"Vahidpour, Shabnam","givenName":"Shabnam","familyName":"Vahidpour","affiliation":[],"nameIdentifiers":[]},{"name":"X. Li, Willis","givenName":"Willis","familyName":"X. Li","affiliation":[],"nameIdentifiers":[]},{"name":"Karijolich, John","givenName":"John","familyName":"Karijolich","affiliation":[],"nameIdentifiers":[]},{"name":"Guo, Dongdong","givenName":"Dongdong","familyName":"Guo","affiliation":[],"nameIdentifiers":[]},{"name":"Pei, Shanshan","givenName":"Shanshan","familyName":"Pei","affiliation":[],"nameIdentifiers":[]},{"name":"Yu, Yi-Tao","givenName":"Yi-Tao","familyName":"Yu","affiliation":[],"nameIdentifiers":[]},{"name":"Zhou, Rui","givenName":"Rui","familyName":"Zhou","affiliation":[],"nameIdentifiers":[]},{"name":"X Li, Willis","givenName":"Willis","familyName":"X Li","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Requirement for CRIF1 in RNA interference and Dicer-2 stability"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2015,"subjects":[{"subject":"Virology"},{"subject":"Hematology"},{"subject":"Mental Health"},{"subject":"Developmental Biology"},{"subject":"Biological Sciences"},{"subject":"Immunology"},{"subject":"FOS: Clinical medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Earth and Environmental Sciences"},{"subject":"Molecular Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Cell Biology"},{"subject":"Microbiology"},{"subject":"Biochemistry"},{"subject":"Biophysics"}],"contributors":[],"dates":[{"date":"2015-12-05","dateType":"Created"},{"date":"2019-08-19","dateType":"Updated"},{"date":"2015","dateType":"Issued"}],"language":null,"types":{"ris":"GEN","bibtex":"misc","citeproc":"article","schemaOrg":"Collection","resourceType":"Collection","resourceTypeGeneral":"Collection"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.c.2026676","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":[],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"RNA interference (RNAi) is a eukaryotic gene-silencing system. Although the biochemistry of RNAi is relatively well defined, how this pathway is regulated remains incompletely understood. To identify genes involved in regulating the RNAi pathway, we screened for genetic mutations in \u003ci\u003eDrosophila\u003c/i\u003e that alter the efficiency of RNAi. We identified the \u003ci\u003eDrosophila\u003c/i\u003e homolog of the mammalian CR6-interacting factor 1 (CRIF1), also known as growth arrest and DNA-damage-inducible 45-gamma interacting protein (Gadd45GIP1), as a potential new regulator of the RNAi pathway. Loss-of-function mutants of \u003ci\u003eDrosophila CRIF1\u003c/i\u003e (\u003ci\u003edCRIF)\u003c/i\u003e are deficient in RNAi-mediated target gene knock-down, in the biogenesis of small interfering RNA (siRNA) molecules, and in antiviral immunity. Moreover, we show that dCRIF may function by interacting with, and stabilizing, the RNase III enzyme Dicer-2. Our results suggest that dCRIF may play an important role in regulating the RNAi pathway.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"PD94bWwgdmVyc2lvbj0iMS4wIiBlbmNvZGluZz0iVVRGLTgiPz4KPHJlc291cmNlIHhtbG5zPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCIgeG1sbnM6eHNpPSJodHRwOi8vd3d3LnczLm9yZy8yMDAxL1hNTFNjaGVtYS1pbnN0YW5jZSIgeHNpOnNjaGVtYUxvY2F0aW9uPSJodHRwOi8vZGF0YWNpdGUub3JnL3NjaGVtYS9rZXJuZWwtNCBodHRwOi8vc2NoZW1hLmRhdGFjaXRlLm9yZy9tZXRhL2tlcm5lbC00LjQvbWV0YWRhdGEueHNkIj4KICA8aWRlbnRpZmllciBpZGVudGlmaWVyVHlwZT0iRE9JIj4xMC42MDg0L005LkZJR1NIQVJFLkMuMjAyNjY3Ni5WMTwvaWRlbnRpZmllcj4KICA8Y3JlYXRvcnM+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlN1IEp1biBMaW08L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPlN1PC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPkp1biBMaW08L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPkFudGhvbnkgU2NvdHQ8L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPkFudGhvbnk8L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+U2NvdHQ8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlhpYW8tUGVuZyBYaW9uZzwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+WGlhby1QZW5nPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPlhpb25nPC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5TaGFibmFtIFZhaGlkcG91cjwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+U2hhYm5hbTwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5WYWhpZHBvdXI8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPldpbGxpcyBYLiBMaTwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+V2lsbGlzPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPlguIExpPC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5Kb2huIEthcmlqb2xpY2g8L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPkpvaG48L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+S2FyaWpvbGljaDwvZmFtaWx5TmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+RG9uZ2RvbmcgR3VvPC9jcmVhdG9yTmFtZT4KICAgICAgPGdpdmVuTmFtZT5Eb25nZG9uZzwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5HdW88L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgICA8Y3JlYXRvcj4KICAgICAgPGNyZWF0b3JOYW1lPlNoYW5zaGFuIFBlaTwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+U2hhbnNoYW48L2dpdmVuTmFtZT4KICAgICAgPGZhbWlseU5hbWU+UGVpPC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5ZaS1UYW8gWXU8L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPllpLVRhbzwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5ZdTwvZmFtaWx5TmFtZT4KICAgIDwvY3JlYXRvcj4KICAgIDxjcmVhdG9yPgogICAgICA8Y3JlYXRvck5hbWU+UnVpIFpob3U8L2NyZWF0b3JOYW1lPgogICAgICA8Z2l2ZW5OYW1lPlJ1aTwvZ2l2ZW5OYW1lPgogICAgICA8ZmFtaWx5TmFtZT5aaG91PC9mYW1pbHlOYW1lPgogICAgPC9jcmVhdG9yPgogICAgPGNyZWF0b3I+CiAgICAgIDxjcmVhdG9yTmFtZT5XaWxsaXMgWCBMaTwvY3JlYXRvck5hbWU+CiAgICAgIDxnaXZlbk5hbWU+V2lsbGlzPC9naXZlbk5hbWU+CiAgICAgIDxmYW1pbHlOYW1lPlggTGk8L2ZhbWlseU5hbWU+CiAgICA8L2NyZWF0b3I+CiAgPC9jcmVhdG9ycz4KICA8dGl0bGVzPgogICAgPHRpdGxlPlJlcXVpcmVtZW50IGZvciBDUklGMSBpbiBSTkEgaW50ZXJmZXJlbmNlIGFuZCBEaWNlci0yIHN0YWJpbGl0eTwvdGl0bGU+CiAgPC90aXRsZXM+CiAgPGRlc2NyaXB0aW9ucz4KICAgIDxkZXNjcmlwdGlvbiBkZXNjcmlwdGlvblR5cGU9IkFic3RyYWN0Ij4mbHQ7ZGl2Jmd0OyZsdDtwJmd0O1JOQSBpbnRlcmZlcmVuY2UgKFJOQWkpIGlzIGEgZXVrYXJ5b3RpYyBnZW5lLXNpbGVuY2luZyBzeXN0ZW0uIEFsdGhvdWdoIHRoZSBiaW9jaGVtaXN0cnkgb2YgUk5BaSBpcyByZWxhdGl2ZWx5IHdlbGwgZGVmaW5lZCwgaG93IHRoaXMgcGF0aHdheSBpcyByZWd1bGF0ZWQgcmVtYWlucyBpbmNvbXBsZXRlbHkgdW5kZXJzdG9vZC4gVG8gaWRlbnRpZnkgZ2VuZXMgaW52b2x2ZWQgaW4gcmVndWxhdGluZyB0aGUgUk5BaSBwYXRod2F5LCB3ZSBzY3JlZW5lZCBmb3IgZ2VuZXRpYyBtdXRhdGlvbnMgaW4gJmx0O2kmZ3Q7RHJvc29waGlsYSZsdDsvaSZndDsgdGhhdCBhbHRlciB0aGUgZWZmaWNpZW5jeSBvZiBSTkFpLiBXZSBpZGVudGlmaWVkIHRoZSAmbHQ7aSZndDtEcm9zb3BoaWxhJmx0Oy9pJmd0OyBob21vbG9nIG9mIHRoZSBtYW1tYWxpYW4gQ1I2LWludGVyYWN0aW5nIGZhY3RvciAxIChDUklGMSksIGFsc28ga25vd24gYXMgZ3Jvd3RoIGFycmVzdCBhbmQgRE5BLWRhbWFnZS1pbmR1Y2libGUgNDUtZ2FtbWEgaW50ZXJhY3RpbmcgcHJvdGVpbiAoR2FkZDQ1R0lQMSksIGFzIGEgcG90ZW50aWFsIG5ldyByZWd1bGF0b3Igb2YgdGhlIFJOQWkgcGF0aHdheS4gTG9zcy1vZi1mdW5jdGlvbiBtdXRhbnRzIG9mICZsdDtpJmd0O0Ryb3NvcGhpbGEgQ1JJRjEmbHQ7L2kmZ3Q7ICgmbHQ7aSZndDtkQ1JJRikmbHQ7L2kmZ3Q7IGFyZSBkZWZpY2llbnQgaW4gUk5BaS1tZWRpYXRlZCB0YXJnZXQgZ2VuZSBrbm9jay1kb3duLCBpbiB0aGUgYmlvZ2VuZXNpcyBvZiBzbWFsbCBpbnRlcmZlcmluZyBSTkEgKHNpUk5BKSBtb2xlY3VsZXMsIGFuZCBpbiBhbnRpdmlyYWwgaW1tdW5pdHkuIE1vcmVvdmVyLCB3ZSBzaG93IHRoYXQgZENSSUYgbWF5IGZ1bmN0aW9uIGJ5IGludGVyYWN0aW5nIHdpdGgsIGFuZCBzdGFiaWxpemluZywgdGhlIFJOYXNlIElJSSBlbnp5bWUgRGljZXItMi4gT3VyIHJlc3VsdHMgc3VnZ2VzdCB0aGF0IGRDUklGIG1heSBwbGF5IGFuIGltcG9ydGFudCByb2xlIGluIHJlZ3VsYXRpbmcgdGhlIFJOQWkgcGF0aHdheS4mbHQ7L3AmZ3Q7Jmx0Oy9kaXYmZ3Q7PC9kZXNjcmlwdGlvbj4KICA8L2Rlc2NyaXB0aW9ucz4KICA8c3ViamVjdHM+CiAgICA8c3ViamVjdD5WaXJvbG9neTwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkhlbWF0b2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5NZW50YWwgSGVhbHRoPC9zdWJqZWN0PgogICAgPHN1YmplY3Q+RGV2ZWxvcG1lbnRhbCBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+QmlvbG9naWNhbCBTY2llbmNlczwvc3ViamVjdD4KICAgIDxzdWJqZWN0PkltbXVub2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5FYXJ0aCBhbmQgRW52aXJvbm1lbnRhbCBTY2llbmNlczwvc3ViamVjdD4KICAgIDxzdWJqZWN0Pk1vbGVjdWxhciBCaW9sb2d5PC9zdWJqZWN0PgogICAgPHN1YmplY3Q+R2VuZXRpY3M8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5DZWxsIEJpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5NaWNyb2Jpb2xvZ3k8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW9jaGVtaXN0cnk8L3N1YmplY3Q+CiAgICA8c3ViamVjdD5CaW9waHlzaWNzPC9zdWJqZWN0PgogIDwvc3ViamVjdHM+CiAgPHB1Ymxpc2hlcj5UYXlsb3IgJmFtcDsgRnJhbmNpczwvcHVibGlzaGVyPgogIDxwdWJsaWNhdGlvblllYXI+MjAxNTwvcHVibGljYXRpb25ZZWFyPgogIDxkYXRlcz4KICAgIDxkYXRlIGRhdGVUeXBlPSJDcmVhdGVkIj4yMDE1LTEyLTA1PC9kYXRlPgogICAgPGRhdGUgZGF0ZVR5cGU9IlVwZGF0ZWQiPjIwMTktMDgtMTk8L2RhdGU+CiAgPC9kYXRlcz4KICA8cmVzb3VyY2VUeXBlIHJlc291cmNlVHlwZUdlbmVyYWw9IkNvbGxlY3Rpb24iPkNvbGxlY3Rpb248L3Jlc291cmNlVHlwZT4KICA8cmVsYXRlZElkZW50aWZpZXJzPgogICAgPHJlbGF0ZWRJZGVudGlmaWVyIHJlbGF0ZWRJZGVudGlmaWVyVHlwZT0iRE9JIiByZWxhdGlvblR5cGU9IklzSWRlbnRpY2FsVG8iPjEwLjYwODQvbTkuZmlnc2hhcmUuYy4yMDI2Njc2PC9yZWxhdGVkSWRlbnRpZmllcj4KICA8L3JlbGF0ZWRJZGVudGlmaWVycz4KICA8cmlnaHRzTGlzdD4KICAgIDxyaWdodHMgcmlnaHRzVVJJPSJodHRwczovL2NyZWF0aXZlY29tbW9ucy5vcmcvbGljZW5zZXMvYnkvNC4wLyI+Q0MgQlkgNC4wPC9yaWdodHM+CiAgPC9yaWdodHNMaXN0Pgo8L3Jlc291cmNlPg==","url":"https://tandf.figshare.com/collections/Requirement_for_CRIF1_in_RNA_interference_and_Dicer_2_stability/2026676/1","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2015-12-05T19:22:56.000Z","registered":"2015-12-05T19:22:57.000Z","published":"2015","updated":"2024-03-21T08:33:47.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.c.2026676.v1","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}