{"data":{"id":"10.6084/m9.figshare.9750602.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.9750602.v1","prefix":"10.6084","suffix":"m9.figshare.9750602.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Müller, Paul","nameType":"Personal","givenName":"Paul","familyName":"Müller","affiliation":[],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-5734-3130","nameIdentifierScheme":"ORCID"}]},{"name":"Schürmann, Mirjam","nameType":"Personal","givenName":"Mirjam","familyName":"Schürmann","affiliation":[],"nameIdentifiers":[]},{"name":"Girardo, Salvatore","nameType":"Personal","givenName":"Salvatore","familyName":"Girardo","affiliation":[],"nameIdentifiers":[]},{"name":"Cojoc, Gheorghe","nameType":"Personal","givenName":"Gheorghe","familyName":"Cojoc","affiliation":[],"nameIdentifiers":[]},{"name":"Guck, Jochen","nameType":"Personal","givenName":"Jochen","familyName":"Guck","affiliation":[],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-1453-6119","nameIdentifierScheme":"ORCID"}]}],"titles":[{"title":"Quantitative phase microscopy images of spherical droplets, beads, and cells"}],"publisher":"figshare","container":{},"publicationYear":2019,"subjects":[{"subject":"Biophysics"},{"subject":"111602 Human Biophysics","subjectScheme":"FOR"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Basic medicine","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2019-08-30","dateType":"Created"},{"date":"2019-08-30","dateType":"Updated"},{"date":"2019","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.9750602","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["367322061 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Zero v1.0 Universal","rightsUri":"https://creativecommons.org/publicdomain/zero/1.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc0-1.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Example data sets for quantitative phase imaging.\u003cbr\u003e\u003cbr\u003eThese data were used by Müller et al. [1] to demonstrate the\u003cbr\u003eaccurate retrieval of size and refractive index for spherical\u003cbr\u003eobjects by means of a two-dimensional phase data fitting\u003cbr\u003ealgorithm.\u003cbr\u003e\u003cbr\u003eAbbreviations:\u003cbr\u003e\u003cbr\u003e - DHM: digital holographic microscopy\u003cbr\u003e - FUS: fused in sarcoma (RNA-binding protein)\u003cbr\u003e - HL60: name of a human leukemia cell line\u003cbr\u003e - QLSI: quadri-wave lateral shearing interferometry\u003cbr\u003e - PAA: Polyacrylamide (hydrogel)\u003cbr\u003e - PBS: phosphate buffered saline (buffer medium)\u003cbr\u003e\u003cbr\u003eThe data are related to [1] according to the following scheme:\u003cbr\u003e\u003cbr\u003eFigure 4:\u003cbr\u003e\u003cbr\u003e a-c) SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07\u003cbr\u003e Silicone oil droplet measured in PBS using QLSI\u003cbr\u003e d-f) SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5\u003cbr\u003e FUS protein droplet measured in egg extract using QLSI\u003cbr\u003e (included in fig. 5a)\u003cbr\u003e g-j) SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23\u003cbr\u003e PAA hydrogel bead (referred to as \"type A\" in [1]) measured\u003cbr\u003e in PBS using QLSI (included in fig. 5b)\u003cbr\u003e k-m) DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11\u003cbr\u003e PAA hydrogel bead (referred to as \"type B\" in [1]) measured\u003cbr\u003e in PBS using DHM\u003cbr\u003e n-q) QLSI_2015-11-09_16-03-52_HL60\u003cbr\u003e HL60 cells measured in PBS using QLSI (included in fig. 5c)\u003cbr\u003e r-s) DHM_2015-11-09_16-48-45_HL60\u003cbr\u003e HL60 cells measured in PBS using DHM (included in fig. 5d)\u003cbr\u003e\u003cbr\u003eFigure 5:\u003cbr\u003e\u003cbr\u003e a) QLSI_2016-04-29_FUS-droplet-780-5\u003cbr\u003e Time lapse measurement of a FUS protein droplet measured in\u003cbr\u003e egg extract using QLSI\u003cbr\u003e b) QLSI_2016-07-05_PAA-beads-S6-A\u003cbr\u003e PAA hydrogel beads (referred to as \"type A\" in [1]) measured\u003cbr\u003e in PBS using QLSI\u003cbr\u003e c) QLSI_2015-11-09_HL60\u003cbr\u003e HL60 cells measured in PBS using QLSI\u003cbr\u003e d) DHM_2015-11-09_HL60\u003cbr\u003e HL60 cells measured in PBS using DHM\u003cbr\u003e\u003cbr\u003eThe data are stored in different file formats:\u003cbr\u003e\u003cbr\u003e - .tif: digital holography data or (!) SID4Bio (Phasics S.A.) data\u003cbr\u003e - .h5: HDF5-based qpimage [2] file format\u003cbr\u003e - .zip: each ZIP file contains several .tif files\u003cbr\u003e - .cfg: meta data and analysis recipe for each data set,\u003cbr\u003e stored as a DryMass [3] profile\u003cbr\u003e\u003cbr\u003eThe phase data can be extracted with DryMass [3] and qpformat [4],\u003cbr\u003ee.g. using the command-line interface of DryMass:\u003cbr\u003e\u003cbr\u003e dm_analyze_sphere --profile DHM_2015-11-09_HL60.cfg DHM_2015-11-09_HL60.zip\u003cbr\u003e\u003cbr\u003eFor more information and a detailed analysis, please refer to [1].\u003cbr\u003e\u003cbr\u003e\u003cbr\u003eLicense: CC0 (https://creativecommons.org/publicdomain/zero/1.0/)\u003cbr\u003e\u003cbr\u003e\u003cbr\u003eSHA256 sums:\u003cbr\u003e\u003cbr\u003ecac55752e36ddf3a1960dc8fe5c1514455da1fb8a7a185e49cd9054d4ac540ea DHM_2015-11-09_16-48-45_HL60.cfg\u003cbr\u003e04eaf59da61934486385f42600aec0a2ffb3f71c93c2b6b6f247ac237088eefb DHM_2015-11-09_16-48-45_HL60.tif\u003cbr\u003ecac55752e36ddf3a1960dc8fe5c1514455da1fb8a7a185e49cd9054d4ac540ea DHM_2015-11-09_HL60.cfg\u003cbr\u003e64e7b0e6fab4d947b82333ea2edd20b41ce51c813c97ae1070da90e58de35b02 DHM_2015-11-09_HL60.zip\u003cbr\u003e685c886d44b9359dca1b2d2bd504f2b1d5f149c05da5a1263ee802c02fb41e66 DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11.cfg\u003cbr\u003e75608e106ecf41a22d707c966faf850f1414e9f0012ce69104658680b4b26876 DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11.tif\u003cbr\u003e138e927853f3c8785eab505a42502a9caadee311e95a6b89a6b9062293e0187b QLSI_2015-11-09_16-03-52_HL60.cfg\u003cbr\u003e73dd8612b713b62227f52e68619f2e5794adabd29c09622b985fdcc842eb68ff QLSI_2015-11-09_16-03-52_HL60.h5\u003cbr\u003e138e927853f3c8785eab505a42502a9caadee311e95a6b89a6b9062293e0187b QLSI_2015-11-09_HL60.cfg\u003cbr\u003e9f3af6ccdd0a71e53d2907ee8f7b6e478c56da99d3dac9a443aceaa4b9f1658e QLSI_2015-11-09_HL60.h5\u003cbr\u003e1a95d8bfcee3e4b442afccd88d0fa89ed9013fe0c96bf5a337ede481eef1f99d QLSI_2016-04-29_FUS-droplet-780-5.cfg\u003cbr\u003ef9a407ca31f3aaa106aa7b5db74ab74b9244e9624212e312ce3c82afa60ef714 QLSI_2016-04-29_FUS-droplet-780-5.zip\u003cbr\u003e3dc230d213d788d194b053c04ed019104207aa631eac27924215232dce0b0773 QLSI_2016-07-05_PAA-beads-S6-A.cfg\u003cbr\u003e7fa1becd016dcd3bf28c8c4c693e15110d239dca803414eed9eb0a62bc5a9daf QLSI_2016-07-05_PAA-beads-S6-A.zip\u003cbr\u003eea8143e609217dc54e2ded3185ff4b88605c995376a4b86cb297a44aa4ae7baf SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5.cfg\u003cbr\u003e5819ec142b58fd7ef9df614a4ac63c78efdaf9ae9e8f02b5ce2f6215fd9251d1 SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5.tif\u003cbr\u003e0b478d422d6510aa4cf8b5d082128c595cac4a4d3b1ce4c53c8b496d410299cf SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23.cfg\u003cbr\u003eab9503b33562968333423b5a541ee41cac135fa1153a835da3c009f9f5c305ed SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23.tif\u003cbr\u003e3bb6ed2275f61d03628e5dbea861b7fe87870cd17cd998679cd14da30aaab274 SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07.cfg\u003cbr\u003ee2b3d79bf3b7904a9184b79746dbeff696f716e6789541ff6835672514a25a93 SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07.tif\u003cbr\u003e\u003cbr\u003e\u003cbr\u003eReferences:\u003cbr\u003e\u003cbr\u003e[1] https://dx.doi.org/10.1364/OE.26.010729\u003cbr\u003e[2] https://qpimage.readthedocs.io\u003cbr\u003e[3] https://drymass.readthedocs.io\u003cbr\u003e[4] https://qpformat.readthedocs.io\u003cbr\u003e\u003cbr\u003e","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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  <identifier identifierType="DOI">10.6084/M9.FIGSHARE.9750602.V1</identifier>
  <creators>
    <creator>
      <creatorName>Paul Müller</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org">0000-0002-5734-3130</nameIdentifier>
    </creator>
    <creator>
      <creatorName>Mirjam Schürmann</creatorName>
    </creator>
    <creator>
      <creatorName>Salvatore Girardo</creatorName>
    </creator>
    <creator>
      <creatorName>Gheorghe Cojoc</creatorName>
    </creator>
    <creator>
      <creatorName>Jochen Guck</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org">0000-0002-1453-6119</nameIdentifier>
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  <titles>
    <title>Quantitative phase microscopy images of spherical droplets, beads, and cells</title>
  </titles>
  <descriptions>
    <description descriptionType="Abstract">Example data sets for quantitative phase imaging.&lt;br&gt;&lt;br&gt;These data were used by Müller et al. [1] to demonstrate the&lt;br&gt;accurate retrieval of size and refractive index for spherical&lt;br&gt;objects by means of a two-dimensional phase data fitting&lt;br&gt;algorithm.&lt;br&gt;&lt;br&gt;Abbreviations:&lt;br&gt;&lt;br&gt;  - DHM: digital holographic microscopy&lt;br&gt;  - FUS: fused in sarcoma (RNA-binding protein)&lt;br&gt;  - HL60: name of a human leukemia cell line&lt;br&gt;  - QLSI: quadri-wave lateral shearing interferometry&lt;br&gt;  - PAA: Polyacrylamide (hydrogel)&lt;br&gt;  - PBS: phosphate buffered saline (buffer medium)&lt;br&gt;&lt;br&gt;The data are related to [1] according to the following scheme:&lt;br&gt;&lt;br&gt;Figure 4:&lt;br&gt;&lt;br&gt; a-c) SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07&lt;br&gt;      Silicone oil droplet measured in PBS using QLSI&lt;br&gt; d-f) SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5&lt;br&gt;      FUS protein droplet measured in egg extract using QLSI&lt;br&gt;      (included in fig. 5a)&lt;br&gt; g-j) SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23&lt;br&gt;      PAA hydrogel bead (referred to as "type A" in [1]) measured&lt;br&gt;      in PBS using QLSI (included in fig. 5b)&lt;br&gt; k-m) DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11&lt;br&gt;      PAA hydrogel bead (referred to as "type B" in [1]) measured&lt;br&gt;      in PBS using DHM&lt;br&gt; n-q) QLSI_2015-11-09_16-03-52_HL60&lt;br&gt;      HL60 cells measured in PBS using QLSI (included in fig. 5c)&lt;br&gt; r-s) DHM_2015-11-09_16-48-45_HL60&lt;br&gt;      HL60 cells measured in PBS using DHM (included in fig. 5d)&lt;br&gt;&lt;br&gt;Figure 5:&lt;br&gt;&lt;br&gt; a) QLSI_2016-04-29_FUS-droplet-780-5&lt;br&gt;    Time lapse measurement of a FUS protein droplet measured in&lt;br&gt;    egg extract using QLSI&lt;br&gt; b) QLSI_2016-07-05_PAA-beads-S6-A&lt;br&gt;    PAA hydrogel beads (referred to as "type A" in [1]) measured&lt;br&gt;    in PBS using QLSI&lt;br&gt; c) QLSI_2015-11-09_HL60&lt;br&gt;    HL60 cells measured in PBS using QLSI&lt;br&gt; d) DHM_2015-11-09_HL60&lt;br&gt;    HL60 cells measured in PBS using DHM&lt;br&gt;&lt;br&gt;The data are stored in different file formats:&lt;br&gt;&lt;br&gt;  - .tif: digital holography data or (!) SID4Bio (Phasics S.A.) data&lt;br&gt;  - .h5: HDF5-based qpimage [2] file format&lt;br&gt;  - .zip: each ZIP file contains several .tif files&lt;br&gt;  - .cfg: meta data and analysis recipe for each data set,&lt;br&gt;    stored as a DryMass [3] profile&lt;br&gt;&lt;br&gt;The phase data can be extracted with DryMass [3] and qpformat [4],&lt;br&gt;e.g. using the command-line interface of DryMass:&lt;br&gt;&lt;br&gt;  dm_analyze_sphere --profile DHM_2015-11-09_HL60.cfg DHM_2015-11-09_HL60.zip&lt;br&gt;&lt;br&gt;For more information and a detailed analysis, please refer to [1].&lt;br&gt;&lt;br&gt;&lt;br&gt;License: CC0 (https://creativecommons.org/publicdomain/zero/1.0/)&lt;br&gt;&lt;br&gt;&lt;br&gt;SHA256 sums:&lt;br&gt;&lt;br&gt;cac55752e36ddf3a1960dc8fe5c1514455da1fb8a7a185e49cd9054d4ac540ea  DHM_2015-11-09_16-48-45_HL60.cfg&lt;br&gt;04eaf59da61934486385f42600aec0a2ffb3f71c93c2b6b6f247ac237088eefb  DHM_2015-11-09_16-48-45_HL60.tif&lt;br&gt;cac55752e36ddf3a1960dc8fe5c1514455da1fb8a7a185e49cd9054d4ac540ea  DHM_2015-11-09_HL60.cfg&lt;br&gt;64e7b0e6fab4d947b82333ea2edd20b41ce51c813c97ae1070da90e58de35b02  DHM_2015-11-09_HL60.zip&lt;br&gt;685c886d44b9359dca1b2d2bd504f2b1d5f149c05da5a1263ee802c02fb41e66  DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11.cfg&lt;br&gt;75608e106ecf41a22d707c966faf850f1414e9f0012ce69104658680b4b26876  DHM_2016-01-15_14-29-06_PAA-beads-S3-B_11.tif&lt;br&gt;138e927853f3c8785eab505a42502a9caadee311e95a6b89a6b9062293e0187b  QLSI_2015-11-09_16-03-52_HL60.cfg&lt;br&gt;73dd8612b713b62227f52e68619f2e5794adabd29c09622b985fdcc842eb68ff  QLSI_2015-11-09_16-03-52_HL60.h5&lt;br&gt;138e927853f3c8785eab505a42502a9caadee311e95a6b89a6b9062293e0187b  QLSI_2015-11-09_HL60.cfg&lt;br&gt;9f3af6ccdd0a71e53d2907ee8f7b6e478c56da99d3dac9a443aceaa4b9f1658e  QLSI_2015-11-09_HL60.h5&lt;br&gt;1a95d8bfcee3e4b442afccd88d0fa89ed9013fe0c96bf5a337ede481eef1f99d  QLSI_2016-04-29_FUS-droplet-780-5.cfg&lt;br&gt;f9a407ca31f3aaa106aa7b5db74ab74b9244e9624212e312ce3c82afa60ef714  QLSI_2016-04-29_FUS-droplet-780-5.zip&lt;br&gt;3dc230d213d788d194b053c04ed019104207aa631eac27924215232dce0b0773  QLSI_2016-07-05_PAA-beads-S6-A.cfg&lt;br&gt;7fa1becd016dcd3bf28c8c4c693e15110d239dca803414eed9eb0a62bc5a9daf  QLSI_2016-07-05_PAA-beads-S6-A.zip&lt;br&gt;ea8143e609217dc54e2ded3185ff4b88605c995376a4b86cb297a44aa4ae7baf  SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5.cfg&lt;br&gt;5819ec142b58fd7ef9df614a4ac63c78efdaf9ae9e8f02b5ce2f6215fd9251d1  SID PHA QLSI_2016-04-29_18-00-30_FUS-droplet-780-5.tif&lt;br&gt;0b478d422d6510aa4cf8b5d082128c595cac4a4d3b1ce4c53c8b496d410299cf  SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23.cfg&lt;br&gt;ab9503b33562968333423b5a541ee41cac135fa1153a835da3c009f9f5c305ed  SID PHA QLSI_2016-07-05_PAA-beads-S6-A_23.tif&lt;br&gt;3bb6ed2275f61d03628e5dbea861b7fe87870cd17cd998679cd14da30aaab274  SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07.cfg&lt;br&gt;e2b3d79bf3b7904a9184b79746dbeff696f716e6789541ff6835672514a25a93  SID PHA QLSI_2017-03-29_silicone-oil-in-PBS_07.tif&lt;br&gt;&lt;br&gt;&lt;br&gt;References:&lt;br&gt;&lt;br&gt;[1] https://dx.doi.org/10.1364/OE.26.010729&lt;br&gt;[2] https://qpimage.readthedocs.io&lt;br&gt;[3] https://drymass.readthedocs.io&lt;br&gt;[4] https://qpformat.readthedocs.io&lt;br&gt;&lt;br&gt;</description>
  </descriptions>
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    <subject>Biophysics</subject>
    <subject schemeURI="http://www.abs.gov.au/ausstats/abs@.nsf/0/6BB427AB9696C225CA2574180004463E" subjectScheme="FOR">111602 Human Biophysics</subject>
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  <publisher>figshare</publisher>
  <publicationYear>2019</publicationYear>
  <dates>
    <date dateType="Created">2019-08-30</date>
    <date dateType="Updated">2019-08-30</date>
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