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A total of 258,056 contigs were assembled with the length of 605 ± 71 (mean ± SD) bp, which were longer than those just using HiSeq data. Nucleotide diversity (π) was 0.00304 with 95% confidence intervals from 0.00303 to 0.00304. Based on the assembled sequences, a total of 59,766 perfect single sequence repeats were detected, with AC/GT being the most common repeat motif and GC being the rarest. Based on this breeding population, 41,736 high-quality single nucleotide polymorphisms and 10,412 polymorphic single sequence repeats were obtained. Validation of amplification by randomly selecting 40 polymorphic single sequence repeats showed a high success rate (85%).","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://figshare.com/articles/RADseq_of_yellow_perch_for_diversity_assess/8667815/3","contentUrl":null,"metadataVersion":4,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2019-07-06T03:11:50.000Z","registered":"2019-07-06T03:11:51.000Z","published":"2019","updated":"2020-08-27T00:38:05.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.8667815.v3","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}