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[1] to demonstrate correlative\u003cbr\u003efluorescence and refractive index tomography using an optofluidic rotation.\u003cbr\u003eFiles named \"sinogram\" contain the raw sinogram data. The sinogram data are\u003cbr\u003estored in the qpimage [2] and flimage [3] file formats and contain all relevant\u003cbr\u003einformation (pixel size [m], wavelength [m], medium index, time [s]) for\u003cbr\u003etomographic reconstruction. Files named \"reference\" hold reference images\u003cbr\u003e(corner of a fluorescent gel) that were used to spatially colocalize the\u003cbr\u003equantitative phase data \"qpi\" to the fluorescence data \"fli\". The samples\u003cbr\u003eare identified by the file name:\u003cbr\u003e\u003cbr\u003e- phantom: Cell-sized object consisting of a Polyacrylamide (PAA) bead\u003cbr\u003e containing fused silica beads; No fluorescence data (fig. 3 in [1])\u003cbr\u003e- hl60: HL60 cell; Fluorescence data shows Hoechst staining (fig. 4 in [1])\u003cbr\u003e- retina-young: Young (P10) mouse retina cell; Fluorescence data shows\u003cbr\u003e Hoechst staining (fig. 5a,b in [1])\u003cbr\u003e- retina-adult: Adult mouse retina cell; Fluorescence data shows Hoechst\u003cbr\u003e staining (fig. 5c,d in [1])\u003cbr\u003e\u003cbr\u003eThe quantitative phase data were recorded with a commercial camera\u003cbr\u003e(SID4Bio, Phasics) and converted from the raw format to the amplitude/phase\u003cbr\u003eformat using proprietary software shipped with the camera. The fluorescence\u003cbr\u003edata were recorded with a CMOS camera. For a successful reproduction of the\u003cbr\u003efigures in [1], the following tasks must be performed\u003cbr\u003e\u003cbr\u003e- spatial and temporal colocalization of the fluorescence and phase data\u003cbr\u003e- bleach correction of the fluorescence data\u003cbr\u003e- correction of the lateral movement during optofluidic rotation\u003cbr\u003e- determination of the rotational axis\u003cbr\u003e- determination of a 2PI rotation interval\u003cbr\u003e- estimation of the non-uniform rotational speed\u003cbr\u003e- tomographic reconstruction using backprojection (fluorescence) and\u003cbr\u003e backpropagation (amplitude/phase)\u003cbr\u003e\u003cbr\u003eFor more information and a detailed analysis, please refer to [1], [4] and [5].\u003cbr\u003e\u003cbr\u003e\u003cbr\u003eSHA256 sums:\u003cbr\u003e032c3c989050a225b296c22da9ac0c5b21ecfd196eb6e9681fce366c360fbc01 hl60_reference_fli.h5\u003cbr\u003e7b1f46fab6245093429f90560b09d0e6aa96b6b616d27bb4401d2421ed0bde1b hl60_reference_qpi.h5\u003cbr\u003e2fa3647f536fba16a985ac155670679bcd19d868a9aa15f03331275ba0bdc686 hl60_sinogram_fli.h5\u003cbr\u003e4f2d797d1781a835f3c4b421a95adb5c5226169a94ea3c175ffebf55b58ad4f7 hl60_sinogram_qpi.h5\u003cbr\u003eaff6aa82367697c75b706bd8b99ede8ff225610293bb1e1f9ef6c8dcd67cbbdf phantom_sinogram_qpi.h5\u003cbr\u003e41495a7acdc6974711dd0c01d7331856b8ec38733178df41addd676e0ba45011 retina-adult_reference_fli.h5\u003cbr\u003ec7efa7530be273b09e0f57632554c7f03c1980bb8af78da2f123a500e5ea4d8a retina-adult_reference_qpi.h5\u003cbr\u003e93f6b2c36be2f03bbf0b5a6a01fff42028a69107fe9bbbc594b4c140b1aefd3c retina-adult_sinogram_fli.h5\u003cbr\u003e4761be77def8c03aa91cdb19f5f7dc5aecc3f234dd0d5f777cb14bda32c4d557 retina-adult_sinogram_qpi.h5\u003cbr\u003e032fc6de9cb9e6889a59cc49f596d3953d0dfa5c59aa65340e571a2fdf6e7692 retina-young_reference_fli.h5\u003cbr\u003e53df795724ec7e7d8bbcd9aefe9d24867d3d60b45d6120191079c6a1907c8f6b retina-young_reference_qpi.h5\u003cbr\u003efb84a8a579505ae3f314a3c1847fb5cc474a3c2ce6327ae46a709c4c782a5a10 retina-young_sinogram_fli.h5\u003cbr\u003e62ec6f2fc373449050d798bb00922fa3e5e4ad803f05a093c76007eb3d27994a retina-young_sinogram_qpi.h5\u003cbr\u003e\u003cbr\u003e\u003cbr\u003e[1] https://doi.org/10.1002/jbio.201700145, https://arxiv.org/abs/1706.00715\u003cbr\u003e[2] https://github.com/RI-imaging/qpimage, https://flimage.readthedocs.io\u003cbr\u003e[3] https://github.com/RI-imaging/flimage, https://qpimage.readthedocs.io\u003cbr\u003e[4] http://dx.doi.org/10.1002/jbio.201800095, https://arxiv.org/abs/1805.06214\u003cbr\u003e[5] http://dx.doi.org/10.1364/OE.26.010729, https://arxiv.org/abs/1803.06410\u003cbr\u003e\u003cbr\u003e","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"<?xml version="1.0" encoding="UTF-8"?>
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      <creatorName>Mirjam Schürmann</creatorName>
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    <creator>
      <creatorName>Gheorghe Cojoc</creatorName>
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    <creator>
      <creatorName>Salvatore Girardo</creatorName>
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      <creatorName>Elke Ulbricht</creatorName>
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      <creatorName>Jochen Guck</creatorName>
      <nameIdentifier nameIdentifierScheme="ORCID" schemeURI="http://orcid.org">0000-0002-1453-6119</nameIdentifier>
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      <creatorName>Paul Müller</creatorName>
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  <titles>
    <title>Example data sets for correlative refractive index and fluorescence tomography with optofluidic rotation</title>
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    <description descriptionType="Abstract">Example data sets for correlative refractive index and fluorescence tomography with optofluidic rotation.&lt;br&gt;&lt;br&gt;The HDF5 files contain the raw tomographic data of four cell-sized samples.&lt;br&gt;These samples were used by Schürmann et al. [1] to demonstrate correlative&lt;br&gt;fluorescence and refractive index tomography using an optofluidic rotation.&lt;br&gt;Files named "sinogram" contain the raw sinogram data. The sinogram data are&lt;br&gt;stored in the qpimage [2] and flimage [3] file formats and contain all relevant&lt;br&gt;information (pixel size [m], wavelength [m], medium index, time [s]) for&lt;br&gt;tomographic reconstruction. Files named "reference" hold reference images&lt;br&gt;(corner of a fluorescent gel) that were used to spatially colocalize the&lt;br&gt;quantitative phase data "qpi" to the fluorescence data "fli". The samples&lt;br&gt;are identified by the file name:&lt;br&gt;&lt;br&gt;- phantom: Cell-sized object consisting of a Polyacrylamide (PAA) bead&lt;br&gt;  containing fused silica beads; No fluorescence data (fig. 3 in [1])&lt;br&gt;- hl60: HL60 cell; Fluorescence data shows Hoechst staining (fig. 4 in [1])&lt;br&gt;- retina-young: Young (P10) mouse retina cell; Fluorescence data shows&lt;br&gt;  Hoechst staining (fig. 5a,b in [1])&lt;br&gt;- retina-adult: Adult mouse retina cell; Fluorescence data shows Hoechst&lt;br&gt;  staining (fig. 5c,d in [1])&lt;br&gt;&lt;br&gt;The quantitative phase data were recorded with a commercial camera&lt;br&gt;(SID4Bio, Phasics) and converted from the raw format to the amplitude/phase&lt;br&gt;format using proprietary software shipped with the camera. The fluorescence&lt;br&gt;data were recorded with a CMOS camera. For a successful reproduction of the&lt;br&gt;figures in [1], the following tasks must be performed&lt;br&gt;&lt;br&gt;- spatial and temporal colocalization of the fluorescence and phase data&lt;br&gt;- bleach correction of the fluorescence data&lt;br&gt;- correction of the lateral movement during optofluidic rotation&lt;br&gt;- determination of the rotational axis&lt;br&gt;- determination of a 2PI rotation interval&lt;br&gt;- estimation of the non-uniform rotational speed&lt;br&gt;- tomographic reconstruction using backprojection (fluorescence) and&lt;br&gt;  backpropagation (amplitude/phase)&lt;br&gt;&lt;br&gt;For more information and a detailed analysis, please refer to [1], [4] and [5].&lt;br&gt;&lt;br&gt;&lt;br&gt;SHA256 sums:&lt;br&gt;032c3c989050a225b296c22da9ac0c5b21ecfd196eb6e9681fce366c360fbc01  hl60_reference_fli.h5&lt;br&gt;7b1f46fab6245093429f90560b09d0e6aa96b6b616d27bb4401d2421ed0bde1b  hl60_reference_qpi.h5&lt;br&gt;2fa3647f536fba16a985ac155670679bcd19d868a9aa15f03331275ba0bdc686  hl60_sinogram_fli.h5&lt;br&gt;4f2d797d1781a835f3c4b421a95adb5c5226169a94ea3c175ffebf55b58ad4f7  hl60_sinogram_qpi.h5&lt;br&gt;aff6aa82367697c75b706bd8b99ede8ff225610293bb1e1f9ef6c8dcd67cbbdf  phantom_sinogram_qpi.h5&lt;br&gt;41495a7acdc6974711dd0c01d7331856b8ec38733178df41addd676e0ba45011  retina-adult_reference_fli.h5&lt;br&gt;c7efa7530be273b09e0f57632554c7f03c1980bb8af78da2f123a500e5ea4d8a  retina-adult_reference_qpi.h5&lt;br&gt;93f6b2c36be2f03bbf0b5a6a01fff42028a69107fe9bbbc594b4c140b1aefd3c  retina-adult_sinogram_fli.h5&lt;br&gt;4761be77def8c03aa91cdb19f5f7dc5aecc3f234dd0d5f777cb14bda32c4d557  retina-adult_sinogram_qpi.h5&lt;br&gt;032fc6de9cb9e6889a59cc49f596d3953d0dfa5c59aa65340e571a2fdf6e7692  retina-young_reference_fli.h5&lt;br&gt;53df795724ec7e7d8bbcd9aefe9d24867d3d60b45d6120191079c6a1907c8f6b  retina-young_reference_qpi.h5&lt;br&gt;fb84a8a579505ae3f314a3c1847fb5cc474a3c2ce6327ae46a709c4c782a5a10  retina-young_sinogram_fli.h5&lt;br&gt;62ec6f2fc373449050d798bb00922fa3e5e4ad803f05a093c76007eb3d27994a  retina-young_sinogram_qpi.h5&lt;br&gt;&lt;br&gt;&lt;br&gt;[1] https://doi.org/10.1002/jbio.201700145, https://arxiv.org/abs/1706.00715&lt;br&gt;[2] https://github.com/RI-imaging/qpimage, https://flimage.readthedocs.io&lt;br&gt;[3] https://github.com/RI-imaging/flimage, https://qpimage.readthedocs.io&lt;br&gt;[4] http://dx.doi.org/10.1002/jbio.201800095, https://arxiv.org/abs/1805.06214&lt;br&gt;[5] http://dx.doi.org/10.1364/OE.26.010729, https://arxiv.org/abs/1803.06410&lt;br&gt;&lt;br&gt;</description>
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