{"data":{"id":"10.6084/m9.figshare.7731161.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.7731161.v1","prefix":"10.6084","suffix":"m9.figshare.7731161.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Ningyong Xu","affiliation":[],"nameIdentifiers":[]},{"name":"Ayers, Linn","nameType":"Personal","givenName":"Linn","familyName":"Ayers","affiliation":[],"nameIdentifiers":[]},{"name":"Pastukh, Viktoriya","nameType":"Personal","givenName":"Viktoriya","familyName":"Pastukh","affiliation":[],"nameIdentifiers":[]},{"name":"Dhananjay T. Tambe","affiliation":[],"nameIdentifiers":[]},{"name":"Alexeyev, Mikhail","nameType":"Personal","givenName":"Mikhail","familyName":"Alexeyev","affiliation":[],"nameIdentifiers":[]},{"name":"Stevens, Troy","nameType":"Personal","givenName":"Troy","familyName":"Stevens","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Supplemental data and data sets for: Extracellular Na+ and Orai1 contribute to endothelial wound healing (Xu et al)"}],"publisher":"figshare","container":{},"publicationYear":2019,"subjects":[{"subject":"111601 Cell Physiology","subjectScheme":"FOR"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Basic medicine","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2019-02-18","dateType":"Created"},{"date":"2019-12-16","dateType":"Updated"},{"date":"2019","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.7731161","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["73865934 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Endothelial migration is necessary for wound healing and angiogenesis. Na\u003csup\u003e+\u003c/sup\u003e has been incriminated as an important cellular signal mediating migration, although the ion channels regulating Na\u003csup\u003e+\u003c/sup\u003e permeation that contribute to migration remain poorly understood. We have shown that Orai1 suppresses both constitutive Na\u003csup\u003e+\u003c/sup\u003e leak and Na\u003csup\u003e+\u003c/sup\u003e entry through store-operated calcium entry channels. Here, we examine the independent and combined contributions of extracellular Na\u003csup\u003e+\u003c/sup\u003e and Orai1 to pulmonary artery endothelial cell (PAEC) wound healing. We found that PAEC monolayers migrated into wound areas and achieved confluence within 24 hours post-scratch. There was a fast healing phase within 5 hours, followed by a slow-healing phase between 5 and 24 hours. Na\u003csup\u003e+\u003c/sup\u003e depletion reduced migration speed by at least 50%. Fewer cells at the migration front formed prominent lamellipodia. Some cells at the leading edge gradually detached from the monolayer. Reduction of Orai1 decreased wound closure speed by 25% within 6 hours. This Orai1-dependence was absent in Na\u003csup\u003e+\u003c/sup\u003e-depleted media and after 6 hours. In a non-scratch cell migration assay, the gap areas were partially sealed within 24 hours. Migration speed was reduced by 75% when extracellular Na\u003csup\u003e+\u003c/sup\u003e was replaced by choline. Orai1 did not contribute to non-scratch cell migration, but extracellular Na\u003csup\u003e+\u003c/sup\u003e did. Overall, extracellular Na\u003csup\u003e+\u003c/sup\u003e and Orai1 together acutely regulate endothelial wound healing. Extracellular Na\u003csup\u003e+\u003c/sup\u003e continuously contributes to wound healing between 6 and 24 hours. Extracellular Na\u003csup\u003e+\u003c/sup\u003e but not Orai1 contributes to cell migration in the absence of injury. Orai1 may function as an injury sensor to acutely stimulate fast wound healing.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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