{"data":{"id":"10.6084/m9.figshare.7713728.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.7713728.v1","prefix":"10.6084","suffix":"m9.figshare.7713728.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Ningyong Xu","affiliation":[],"nameIdentifiers":[]},{"name":"Ayers, Linn","nameType":"Personal","givenName":"Linn","familyName":"Ayers","affiliation":[],"nameIdentifiers":[]},{"name":"Pastukh, Viktoriya","nameType":"Personal","givenName":"Viktoriya","familyName":"Pastukh","affiliation":[],"nameIdentifiers":[]},{"name":"Dhananjay T. Tambe","affiliation":[],"nameIdentifiers":[]},{"name":"Alexeyev, Mikhail","nameType":"Personal","givenName":"Mikhail","familyName":"Alexeyev","affiliation":[],"nameIdentifiers":[]},{"name":"Stevens, Troy","nameType":"Personal","givenName":"Troy","familyName":"Stevens","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Supplemental data from: Extracellular Na+ and Orai1 contribute to endothelial wound healing"}],"publisher":"figshare","container":{},"publicationYear":2019,"subjects":[{"subject":"111601 Cell Physiology","subjectScheme":"FOR"},{"subject":"FOS: Basic medicine","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Basic medicine","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2019-02-14","dateType":"Created"},{"date":"2019-02-14","dateType":"Updated"},{"date":"2019","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.7713728","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["71402226 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Endothelial migration is necessary for wound healing and angiogenesis. Na\u003csup\u003e+\u003c/sup\u003e has been incriminated as an important cellular signal mediating migration, although the ion channels regulating Na\u003csup\u003e+\u003c/sup\u003e permeation that contribute to migration remain poorly understood. We have shown that Orai1 suppresses both constitutive Na\u003csup\u003e+\u003c/sup\u003e leak and Na\u003csup\u003e+\u003c/sup\u003e entry through store-operated calcium entry channels. Here, we examine the independent and combined contributions of extracellular Na\u003csup\u003e+\u003c/sup\u003e and Orai1 to pulmonary artery endothelial cell (PAEC) wound healing. We found that PAEC monolayers migrated into wound areas and achieved confluence within 24 hours post-scratch. There was a fast healing phase within 5 hours, followed by a slow-healing phase between 5 and 24 hours. Na\u003csup\u003e+\u003c/sup\u003e depletion reduced migration speed by at least 50%. Fewer cells at the migration front formed prominent lamellipodia. Some cells at the leading edge gradually detached from the monolayer. Reduction of Orai1 decreased wound closure speed by 25% within 6 hours. This Orai1-dependence was absent in Na\u003csup\u003e+\u003c/sup\u003e-depleted media and after 6 hours. In a non-scratch cell migration assay, the gap areas were partially sealed within 24 hours. Migration speed was reduced by 75% when extracellular Na\u003csup\u003e+\u003c/sup\u003e was replaced by choline. Orai1 did not contribute to non-scratch cell migration, but extracellular Na\u003csup\u003e+\u003c/sup\u003e did. Overall, extracellular Na\u003csup\u003e+\u003c/sup\u003e and Orai1 together acutely regulate endothelial wound healing. Extracellular Na\u003csup\u003e+\u003c/sup\u003e continuously contributes to wound healing between 6 and 24 hours. Extracellular Na\u003csup\u003e+\u003c/sup\u003e but not Orai1 contributes to cell migration in the absence of injury. Orai1 may function as an injury sensor to acutely stimulate fast wound healing.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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