{"data":{"id":"10.6084/m9.figshare.7676303.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.7676303.v1","prefix":"10.6084","suffix":"m9.figshare.7676303.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Adejanildo Da Silva Pereira","affiliation":[],"nameIdentifiers":[]},{"name":"Fontan, Rafael Ilhéu Da Costa","nameType":"Personal","givenName":"Rafael Ilhéu Da Costa","familyName":"Fontan","affiliation":[],"nameIdentifiers":[]},{"name":"Franco, Marcelo","nameType":"Personal","givenName":"Marcelo","familyName":"Franco","affiliation":[],"nameIdentifiers":[]},{"name":"Evaldo Cardozo De Souza Júnior","affiliation":[],"nameIdentifiers":[]},{"name":"Cristiane Martins Veloso","affiliation":[],"nameIdentifiers":[]},{"name":"Sampaio, Vanessa Santos","nameType":"Personal","givenName":"Vanessa Santos","familyName":"Sampaio","affiliation":[],"nameIdentifiers":[]},{"name":"Bonomo, Paulo","nameType":"Personal","givenName":"Paulo","familyName":"Bonomo","affiliation":[],"nameIdentifiers":[]},{"name":"Bonomo, Renata Cristina Ferreira","nameType":"Personal","givenName":"Renata Cristina Ferreira","familyName":"Bonomo","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"STUDY OF ALPHA-AMYLASE OBTAINED BY SOLID STATE FERMENTATION OF CASSAVA RESIDUE IN AQUEOUS TWO-PHASE SYSTEMS"}],"publisher":"SciELO journals","container":{},"publicationYear":2019,"subjects":[{"subject":"90499 Chemical Engineering not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Chemical engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical engineering","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2019-02-06","dateType":"Created"},{"date":"2019-02-06","dateType":"Updated"},{"date":"2019","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1590/0104-6632.20180353s20170003","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.7676303","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1381474 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Abstract The α-amylase enzyme was produced by solid state fermentation (SSF) and its partition behavior in Aqueous Two-Phase Systems (ATPS) was determined using systems consisting of polyethylene glycol (PEG) and potassium phosphate buffer. To determine the best conditions for α-amylase production, a central composite design was constructed for two independent variables: fermentation time and moisture content, and for the partition study, a face-centered central composite design (CCF) with four independent variables: PEG molecular weight, pH, temperature and partition time. It was indicated that the best enzyme production conditions were a fermentation time of 35.0 hours and 45.7% moisture content. From the results obtained, it was found that increasing the polymer molecular weight promotes a reduction of the enzyme concentration in the upper phase, increasing its concentration in the lower phase. It was also observed that the partition coefficients increased with increasing pH values and a reduction in temperature.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://scielo.figshare.com/articles/STUDY_OF_ALPHA-AMYLASE_OBTAINED_BY_SOLID_STATE_FERMENTATION_OF_CASSAVA_RESIDUE_IN_AQUEOUS_TWO-PHASE_SYSTEMS/7676303/1","contentUrl":null,"metadataVersion":2,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citati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