{"data":{"id":"10.6084/m9.figshare.7369964.v1","type":"dois","attributes":{"doi":"10.6084/m9.figshare.7369964.v1","prefix":"10.6084","suffix":"m9.figshare.7369964.v1","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Santillan, Ezequiel","nameType":"Personal","givenName":"Ezequiel","familyName":"Santillan","affiliation":[],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-0258-9037","nameIdentifierScheme":"ORCID"}]},{"name":"Seshan, Hari","nameType":"Personal","givenName":"Hari","familyName":"Seshan","affiliation":[],"nameIdentifiers":[]},{"name":"Constancias, Florentin","nameType":"Personal","givenName":"Florentin","familyName":"Constancias","affiliation":[],"nameIdentifiers":[]},{"name":"Drautz-Moses, Daniela I.","nameType":"Personal","givenName":"Daniela I.","familyName":"Drautz-Moses","affiliation":[],"nameIdentifiers":[]},{"name":"Wuertz, Stefan","nameType":"Personal","givenName":"Stefan","familyName":"Wuertz","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Sludge bioreactor microcosm study on complex bacterial communities and ecosystem function using 3-CA disturbance at varying frequencies for 35 days"}],"publisher":"figshare","container":{},"publicationYear":2019,"subjects":[{"subject":"60202 Community Ecology (excl. Invasive Species Ecology)","subjectScheme":"FOR"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Ecology"},{"subject":"60504 Microbial Ecology","subjectScheme":"FOR"},{"subject":"Molecular Biology"},{"subject":"90409 Wastewater Treatment Processes","subjectScheme":"FOR"},{"subject":"FOS: Chemical engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Chemical engineering","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"90799 Environmental Engineering not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Environmental engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Environmental engineering","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"10406 Stochastic Analysis and Modelling","subjectScheme":"FOR"},{"subject":"FOS: Mathematics","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Mathematics","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"10402 Biostatistics","subjectScheme":"FOR"},{"subject":"Statistics"},{"subject":"50102 Ecosystem Function","subjectScheme":"FOR"},{"subject":"FOS: Earth and related environmental sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Earth and related environmental sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"50202 Conservation and Biodiversity","subjectScheme":"FOR"}],"contributors":[],"dates":[{"date":"2019-02-12","dateType":"Created"},{"date":"2020-08-13","dateType":"Updated"},{"date":"2019","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1038/s41522-019-0079-4","relatedIdentifierType":"DOI"},{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.7369964","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["6931039 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Disturbance is known to affect ecosystem structure, but predicting its outcomes remains elusive. Similarly, community diversity is believed to relate to ecosystem functions, yet the underlying mechanisms are poorly understood. Here, we tested the effect of disturbance on the structure, assembly, and ecosystem function of complex microbial communities within an engineered system. We carried out a microcosm experiment where activated sludge bioreactors operated in daily cycles were subjected to eight different frequency levels of augmentation with a toxic pollutant, from never (undisturbed) to everyday (press-disturbed), for 35 days. Microbial communities were assessed by combining distance-based methods, general linear multivariate models, a-diversity indices, and null model analyses on metagenomics and 16S rRNA gene amplicon data. A stronger temporal decrease in a-diversity at the extreme, undisturbed and press-disturbed, ends of the disturbance range led to a hump-backed pattern, with the highest diversity found at intermediate levels of disturbance. Undisturbed and press-disturbed levels displayed the highest community and functional similarity across replicates, suggesting deterministic processes were dominating. The opposite was observed amongst intermediately disturbed levels, indicating stronger stochastic assembly mechanisms. Trade-offs were observed in ecosystem function between organic carbon removal and both nitrification and biomass productivity, as well as between diversity and these functions. Hence, not every ecosystem function was favoured by higher community diversity. Our results show that the assessment of changes in diversity, along with the underlying stochastic-deterministic assembly processes, is essential to understanding the impact of disturbance in complex microbial communities.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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