{"data":{"id":"10.6084/m9.figshare.724793","type":"dois","attributes":{"doi":"10.6084/m9.figshare.724793","prefix":"10.6084","suffix":"m9.figshare.724793","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Mobasheri, Ali","nameType":"Personal","givenName":"Ali","familyName":"Mobasheri","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Effect of curcumin on prostaglandin E2, matrix metalloproteinase-3 and proteoglycan release in articular cartilage"}],"publisher":"f1000research.com","container":{},"publicationYear":2013,"subjects":[{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Toxicology"}],"contributors":[],"dates":[{"date":"2013-06-19","dateType":"Created"},{"date":"2013-07-08","dateType":"Updated"},{"date":"2013","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.12688/f1000research.2-147.v1","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["4879647 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 2% penicillin/streptomycin and no curcumin (control). Green staining (calcein AM) indicates live metabolizing cells. Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 2% penicillin/streptomycin and 12.5 µM curcumin. Green staining (calcein AM) indicates live metabolizing cells and red staining (ethidium homodimer-1) highlights the nuclei of dead cells. Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 2% penicillin/streptomycin and 25 µM curcumin. Green staining (calcein AM) indicates live metabolizing cells. Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 10ng/ml IL-1β, 2% penicillin/streptomycin and 100 µM curcumin. Green staining (calcein AM) indicates live metabolizing cells and red staining (ethidium homodimer-1) highlights the nuclei of dead cells. Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 2% penicillin/streptomycin and 100 µM curcumin. Green staining (calcein AM) indicates live metabolizing cells and red staining (ethidium homodimer-1) highlights the nuclei of dead cells. Three-dimensional confocal reconstruction of z stacks through equine cartilage explants (15μm sections) after five days in culture. Cells were cultured in media with 2% penicillin/streptomycin and sodium nitroprusside (positive control). Green staining (calcein AM) indicates live metabolizing cells and red staining (ethidium homodimer-1) highlights the nuclei of dead cells. Cytotoxicity values of various compounds on equine chondrocytes. Values refer to percentage of dead cells counted. SNP – sodium nitroprusside; NSAID - carprofen; DMSO - dimethyl sulfoxide. Micromolar values refer to curcumin concentrations. Days refer to the time cell death was measured after compound exposure. Proteoglycan (GAG) and supernatant release (uM/ml) of equine cartilage explants in response to various compounds. SNP – sodium nitroprusside; IL-β – interleukin beta. Micromolar values refer to curcumin concentrations. Quantification of matrix metalloproteinase (MMP-3) blots in three different samples of equine cartilage explant secretome (culture supernatants). Effect of various compounds on proteoglycan (GAG) release (uM/ml) in three equine cartilage explants. SNP – sodium nitroprusside; NSAID - carprofen; DMSO - dimethyl sulfoxide. Micromolar values refer to curcumin concentrations. Effect of various compounds on prostaglandin (PGE) and proteoglycan (GAG) release (uM/ml) in three IL-β stimulated equine cartilage explants. SNP – sodium nitroprusside; NSAID - carprofen; DMSO - dimethyl sulfoxide. Micromolar values refer to curcumin concentrations.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://f1000.figshare.com/articles/dataset/Effect_of_curcumin_on_prostaglandin_E2_matrix_metalloproteinase_3_and_proteoglycan_release_in_articular_cartilage/724793","contentUrl":null,"metadataVersion":3,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,"viewCount":0,"viewsOverTime":[],"downloadCount":0,"downloadsOverTime":[],"referenceCount":0,"citationCount":0,"citationsOverTime":[],"partCount":0,"partOfCount":0,"versionCount":0,"versionOfCount":0,"created":"2013-08-24T13:38:09.000Z","registered":"2013-08-24T13:38:10.000Z","published":"2013","updated":"2020-09-05T01:52:18.000Z"},"relationships":{"client":{"data":{"id":"figshare.ars","type":"clients"}},"provider":{"data":{"id":"otjm","type":"providers"}},"media":{"data":{"id":"10.6084/m9.figshare.724793","type":"media"}},"references":{"data":[]},"citations":{"data":[]},"parts":{"data":[]},"partOf":{"data":[]},"versions":{"data":[]},"versionOf":{"data":[]}}}}