{"data":{"id":"10.6084/m9.figshare.6827201.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.6827201.v2","prefix":"10.6084","suffix":"m9.figshare.6827201.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Johnson, Lisa","nameType":"Personal","givenName":"Lisa","familyName":"Johnson","affiliation":[],"nameIdentifiers":[{"schemeUri":"https://orcid.org","nameIdentifier":"https://orcid.org/0000-0002-3600-7218","nameIdentifierScheme":"ORCID"}]},{"name":"Whitehead, Andrew","nameType":"Personal","givenName":"Andrew","familyName":"Whitehead","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"De novo assemblies, annotations, and gene expression profiling of gill epithelium from 16 species of Fundulus killifish in response to salinity change."}],"publisher":"figshare","container":{},"publicationYear":2018,"subjects":[{"subject":"70405 Fish Physiology and Genetics","subjectScheme":"FOR"},{"subject":"FOS: Agriculture, forestry and fisheries","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Agriculture, forestry and fisheries","subjectScheme":"Fields of Science and Technology (FOS)"}],"contributors":[],"dates":[{"date":"2018-07-17","dateType":"Created"},{"date":"2018-07-17","dateType":"Updated"},{"date":"2018","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsIdenticalTo","relatedIdentifier":"10.6084/m9.figshare.6827201","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["1216388 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"P51\u003cbr\u003eIon and Acid-Base Regulation in Fish Symposium#\u003cstrong\u003eICBF2018\u003c/strong\u003e\u003cbr\u003e13th International Congress on the Biology of FishCalgary, Alberta, July 15-19, 2018\u003cbr\u003eEstuaries are characterized by periodic fluxes in salinity. Many species of North American killifish (Fundulus) are estuarine specialists and harbor euryhaline phenotypes. Three clades within Fundulus independently radiated into freshwater environments and have lost their abilities to tolerate high salinity. We use Fundulus as a comparative model system for studying the physiological and genetic mechanisms that diverge between euryhaline and freshwater species. We examined 16 estuarine and freshwater species with representation from each of three clades. Fish from all species were acclimated to either brackish or fresh water then exposed to an acute brackish water challenge. Gill transcriptome data were collected. To enable multi-species comparisons, reference transcriptome assemblies were generated de novo for each species then used to analyze transcriptional responses to salinity change by clade and physiology. We find differences in the gene expression between euryhaline and freshwater species, some of which are shared across clades, implicating molecular mechanisms that contribute to divergent osmoregulatory physiologies.\u003cbr\u003e\u003cbr\u003eFundulus Multispecies Osmotic Transcriptome Sequencing Project (FMOTSP)\u003cbr\u003ehttps://doi.org/10.17605/OSF.IO/M4XEG\u003cbr\u003eAnalysis scripts:https://github.com/ljcohen/RNAseq_15killifish\u003cbr\u003eRaw data:https://www.ncbi.nlm.nih.gov/bioproject/473009\u003cbr\u003e","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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