{"data":{"id":"10.6084/m9.figshare.5772438","type":"dois","attributes":{"doi":"10.6084/m9.figshare.5772438","prefix":"10.6084","suffix":"m9.figshare.5772438","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Giannini Paszinick Apati","affiliation":[],"nameIdentifiers":[]},{"name":"Maikon Kelbert","affiliation":[],"nameIdentifiers":[]},{"name":"Sombrio, Bruna Regina","nameType":"Personal","givenName":"Bruna Regina","familyName":"Sombrio","affiliation":[],"nameIdentifiers":[]},{"name":"Schneider, Andrea Lima Dos Santos","nameType":"Personal","givenName":"Andrea Lima Dos Santos","familyName":"Schneider","affiliation":[],"nameIdentifiers":[]},{"name":"Garcia, Michele Cristina Formolo","nameType":"Personal","givenName":"Michele Cristina Formolo","familyName":"Garcia","affiliation":[],"nameIdentifiers":[]},{"name":"Agenor Furigo Junior","affiliation":[],"nameIdentifiers":[]},{"name":"Pezzin, Ana Paula Testa","nameType":"Personal","givenName":"Ana Paula Testa","familyName":"Pezzin","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Evaluation of the addition of glycerol to Cupriavidus necator culture medium over Poly(3-hydroxybutyrate) production"}],"publisher":"SciELO journals","container":{},"publicationYear":2018,"subjects":[{"subject":"90502 Construction Engineering","subjectScheme":"FOR"},{"subject":"FOS: Civil engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Civil engineering","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"91207 Metals and Alloy Materials","subjectScheme":"FOR"},{"subject":"FOS: Materials engineering","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Materials engineering","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"91299 Materials Engineering not elsewhere classified","subjectScheme":"FOR"}],"contributors":[],"dates":[{"date":"2018-01-10","dateType":"Created"},{"date":"2018-01-10","dateType":"Updated"},{"date":"2018","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1590/s1517-707620170005.0243","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["116344 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"ABSTRACT Glycerol was used as a source of additional carbon in the production of Poly(3-hydroxybutyrate) (P(3HB)). The inverted sugar and glycerol concentrations and the temperature of the Cupriavidus necator culture medium were evaluated using a Central Composite Rotational Design (CCRD). The results showed that the increase in temperature and sugar concentration led to an increase in production and P(3HB) accumulation and when 15 g L-1 of glycerol was added better results were obtained, however these were not considered statistically significant. The best results were obtained at 38 °C and with 30 g L-1 of inverted sugar. Although not considered statistically significant, the addition of 15 g L-1 of glycerol increased the P(3HB) accumulation percentage by 15 %, thus in kinetic terms, greater productivity was obtained in 0.32 g L-1 h-1 polymer. Larger scale assays are being conducted to verify if the addition of glycerol improves the thermal and mechanical properties of the synthesized polymer.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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","url":"https://scielo.figshare.com/articles/Evaluation_of_the_addition_of_glycerol_to_Cupriavidus_necator_culture_medium_over_Poly_3-hydroxybutyrate_production/5772438","contentUrl":null,"metadataVersion":1,"schemaVersion":"http://datacite.org/schema/kernel-4","source":"mds","isActive":true,"state":"findable","reason":null,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