{"data":{"id":"10.6084/m9.figshare.3840708","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3840708","prefix":"10.6084","suffix":"m9.figshare.3840708","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Jin, Hee","nameType":"Personal","givenName":"Hee","familyName":"Jin","affiliation":[],"nameIdentifiers":[]},{"name":"Seulgi Jeon","affiliation":[],"nameIdentifiers":[]},{"name":"Ga-Young Kang","affiliation":[],"nameIdentifiers":[]},{"name":"Hae-June Lee","affiliation":[],"nameIdentifiers":[]},{"name":"Jaeho Cho","affiliation":[],"nameIdentifiers":[]},{"name":"Yun-Sil Lee","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Identification of radiation response genes and proteins from mouse pulmonary tissues after high-dose per fraction irradiation of limited lung volumes"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"Biochemistry"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Medicine"},{"subject":"Cell Biology"},{"subject":"Genetics"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Molecular Biology"},{"subject":"Physiology"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Developmental Biology"},{"subject":"Cancer"},{"subject":"Plant Biology"}],"contributors":[],"dates":[{"date":"2016-09-20","dateType":"Created"},{"date":"2017-01-31","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/09553002.2017.1235297","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["6772860 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"\u003cb\u003ePurpose:\u003c/b\u003e The molecular effects of focal exposure of limited lung volumes to high-dose per fraction irradiation (HDFR) such as stereotactic body radiotherapy (SBRT) have not been fully characterized. In this study, we used such an irradiation system and identified the genes and proteins after HDFR to mouse lung, similar to those associated with human therapy. \u003cb\u003eMethods and materials:\u003c/b\u003e High focal radiation (90 Gy) was applied to a 3-mm volume of the left lung of C57BL6 mice using a small-animal stereotactic irradiator. As well as histological examination for lungs, a cDNA micro array using irradiated lung tissues and a protein array of sera were performed until 4 weeks after irradiation, and radiation-responsive genes and proteins were identified. For comparison, the long-term effects (12 months) of 20 Gy radiation wide-field dose to the left lung were also investigated. \u003cb\u003eResults:\u003c/b\u003e The genes \u003ci\u003eermap\u003c/i\u003e, \u003ci\u003eepb4.2\u003c/i\u003e, \u003ci\u003ecd200r3\u003c/i\u003e (up regulation) and \u003ci\u003ekrt15\u003c/i\u003e, \u003ci\u003ehoxc4\u003c/i\u003e, \u003ci\u003egdf2\u003c/i\u003e, \u003ci\u003ecst9\u003c/i\u003e, \u003ci\u003ecidec\u003c/i\u003e, and \u003ci\u003ebnc1\u003c/i\u003e (down-regulation) and the proteins of AIF, laminin, bNOS, HSP27, β-amyloid (upregulation), and calponin (downregulation) were identified as being responsive to 90 Gy HDFR. The \u003ci\u003egdf2\u003c/i\u003e, \u003ci\u003ecst9\u003c/i\u003e, and \u003ci\u003ecidec\u003c/i\u003e genes also responded to 20 Gy, suggesting that they are universal responsive genes in irradiated lungs. No universal proteins were identified in both 90 Gy and 20 Gy. Calponin, which was downregulated in protein antibody array analysis, showed a similar pattern in microarray data, suggesting a possible HDFR responsive serum biomarker that reflects gene alteration of irradiated lung tissue. These genes and proteins also responded to the lower doses of 20 Gy and 50 Gy HDFR. \u003cb\u003eConclusions:\u003c/b\u003e These results suggest that identified candidate genes and proteins are HDFR-specifically expressed in lung damage induced by HDFR relevant to SBRT in humans.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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