{"data":{"id":"10.6084/m9.figshare.3826725.v2","type":"dois","attributes":{"doi":"10.6084/m9.figshare.3826725.v2","prefix":"10.6084","suffix":"m9.figshare.3826725.v2","identifiers":[],"alternateIdentifiers":[],"creators":[{"name":"Fykerud, Tone A.","nameType":"Personal","givenName":"Tone A.","familyName":"Fykerud","affiliation":[],"nameIdentifiers":[]},{"name":"Knudsen, Lars M.","nameType":"Personal","givenName":"Lars M.","familyName":"Knudsen","affiliation":[],"nameIdentifiers":[]},{"name":"Totland, Max Z.","nameType":"Personal","givenName":"Max Z.","familyName":"Totland","affiliation":[],"nameIdentifiers":[]},{"name":"Sørensen, Vigdis","nameType":"Personal","givenName":"Vigdis","familyName":"Sørensen","affiliation":[],"nameIdentifiers":[]},{"name":"Dahal-Koirala, Shiva","nameType":"Personal","givenName":"Shiva","familyName":"Dahal-Koirala","affiliation":[],"nameIdentifiers":[]},{"name":"Lothe, Ragnhild A.","nameType":"Personal","givenName":"Ragnhild A.","familyName":"Lothe","affiliation":[],"nameIdentifiers":[]},{"name":"Brech, Andreas","nameType":"Personal","givenName":"Andreas","familyName":"Brech","affiliation":[],"nameIdentifiers":[]},{"name":"Leithe, Edward","nameType":"Personal","givenName":"Edward","familyName":"Leithe","affiliation":[],"nameIdentifiers":[]}],"titles":[{"title":"Mitotic cells form actin-based bridges with adjacent cells to provide intercellular communication during rounding"}],"publisher":"Taylor \u0026 Francis","container":{},"publicationYear":2016,"subjects":[{"subject":"Biophysics"},{"subject":"29999 Physical Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"FOS: Physical sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Physical sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Medicine"},{"subject":"Cell Biology"},{"subject":"Physiology"},{"subject":"FOS: Biological sciences","schemeUri":"http://www.oecd.org/science/inno/38235147.pdf","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"FOS: Biological sciences","subjectScheme":"Fields of Science and Technology (FOS)"},{"subject":"Pharmacology"},{"subject":"69999 Biological Sciences not elsewhere classified","subjectScheme":"FOR"},{"subject":"Developmental Biology"},{"subject":"Cancer"}],"contributors":[],"dates":[{"date":"2016-09-27","dateType":"Created"},{"date":"2016-10-10","dateType":"Updated"},{"date":"2016","dateType":"Issued"}],"language":null,"types":{"ris":"DATA","bibtex":"misc","citeproc":"dataset","schemaOrg":"Dataset","resourceType":"Dataset","resourceTypeGeneral":"Dataset"},"relatedIdentifiers":[{"relationType":"IsSupplementTo","relatedIdentifier":"10.1080/15384101.2016.1231280","relatedIdentifierType":"DOI"},{"relationType":"IsPreviousVersionOf","relatedIdentifier":"10.6084/m9.figshare.3826725","relatedIdentifierType":"DOI"}],"relatedItems":[],"sizes":["149374585 Bytes"],"formats":[],"version":null,"rightsList":[{"rights":"Creative Commons Attribution 4.0 International","rightsUri":"https://creativecommons.org/licenses/by/4.0/legalcode","schemeUri":"https://spdx.org/licenses/","rightsIdentifier":"cc-by-4.0","rightsIdentifierScheme":"SPDX"}],"descriptions":[{"description":"In order to achieve accurate chromosome segregation, eukaryotic cells undergo a dramatic change in morphology to obtain a spherical shape during mitosis. Interphase cells communicate directly with each other by exchanging ions and small molecules via gap junctions, which have important roles in controlling cell growth and differentiation. As cells round up during mitosis, the gap junctional communication between mitotic cells and adjacent interphase cells ceases. Whether mitotic cells use alternative mechanisms for mediating direct cell-cell communication during rounding is currently unknown. Here, we have studied the mechanisms involved in the remodeling of gap junctions during mitosis. We further demonstrate that mitotic cells are able to form actin-based plasma membrane bridges with adjacent cells during rounding. These structures, termed “mitotic nanotubes,” were found to be involved in mediating the transport of cytoplasm, including Rab11-positive vesicles, between mitotic cells and adjacent cells. Moreover, a subpool of the gap-junction channel protein connexin43 localized in these intercellular bridges during mitosis. Collectively, the data provide new insights into the mechanisms involved in the remodeling of gap junctions during mitosis and identify actin-based plasma membrane bridges as a novel means of communication between mitotic cells and adjacent cells during rounding.","descriptionType":"Abstract"}],"geoLocations":[],"fundingReferences":[],"xml":"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